FastQCFastQC Report
Wed 25 May 2016
SRR2088394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759232
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT224321.2751018626309663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221171.257196322031432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178161.0127146391152504No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83490.47458209036670546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74470.42330971696740394No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51770.2942761386786962No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT49410.280861193975553No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT48720.2769390279394645No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47550.27028839857392317No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41520.2360120779976717No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG36000.2046347497089639No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35580.20224734429569266No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT31810.18081753856228172No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.1656973042782305No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26510.15069075596623982No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25620.14563173020954598No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25010.14216430806169966No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT23040.1309662398137369No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA21390.12158714711874273No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.11095750873108265No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG18400.10459109429569266No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG17820.10129420110593712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.028.5778221
TCGGATA400.00193159223.1250025
GTATTGG5150.020.1165071
ATTGGAC5000.019.983
GACGGAC1950.018.974367
GTATCAA83550.017.5810892
CGCAAGA2450.017.3673482
CAATGCG1107.813396E-716.81818219
TTGGACC8500.016.323534
ACGGACC2400.016.18758
ATTAGAC1151.2430355E-616.0869563
GTCCTAT1403.4762706E-815.8571431
TGCGACG1059.348656E-615.85714222
CGGACCA2350.015.7446819
TCTATAC1659.749783E-1015.696973
GGACCCT9100.015.4505496
CGGTCCA855.366161E-415.23529410
CAAGACG3300.015.1363644
CGAACGA750.00410555714.816
GTACTAG2400.014.6458341
Produced by FastQC (version 0.11.5)