FastQCFastQC Report
Wed 25 May 2016
SRR2088393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177438
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT297340.9357853717366005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285550.8986800057152964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259340.8161921648825248No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG123720.38937030399963746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.31283065161302914No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70270.22115301699041806No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66580.2095398871669565No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT64040.2015460254456578No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT61350.19308008527625087No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA53770.16922438769851686No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT51480.16201732339073177No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG46100.14508544305191792No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43790.13781543495105178No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39310.12371602530088707No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39150.12321247495623833No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.11839727478553477No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34470.10848362737526272No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT32680.10285015789450495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.028.1251751
ATTGGAC10300.018.6796133
GTATTGG11600.017.2241381
TGGACCC15500.016.3516125
GTATCAA124200.016.2210162
GGACCCT15350.016.0293166
TTGGACC15950.015.8902834
CGAACGA2500.014.80000116
ACGAACG2650.014.66037715
CGTCCCG908.2796824E-414.38888832
GTCGGGT1803.3378456E-914.38888824
TATTGGA15000.014.0600012
CGTCTAT1458.9234527E-714.0344831
TAGGTCG1854.9076334E-914.021
GATAACG2650.013.96226311
AACGGTA1203.303963E-513.87500118
GACCCTC17950.013.8105847
ATAACGA2750.013.45454512
GTCTATA2651.8189894E-1213.264151
ACCCTCG18450.013.1355028
Produced by FastQC (version 0.11.5)