##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3177438
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386729811879885	31.0	31.0	34.0	30.0	34.0
2	31.608328785644282	31.0	31.0	34.0	30.0	34.0
3	31.69819898924857	31.0	31.0	34.0	30.0	34.0
4	35.48790472072154	37.0	35.0	37.0	33.0	37.0
5	35.29942645615745	37.0	35.0	37.0	33.0	37.0
6	35.3349129078207	37.0	35.0	37.0	32.0	37.0
7	35.22373874801019	37.0	35.0	37.0	32.0	37.0
8	35.237059857658906	37.0	35.0	37.0	32.0	37.0
9	36.76109022426244	39.0	37.0	39.0	32.0	39.0
10	36.630205845086515	39.0	35.0	39.0	32.0	39.0
11	36.742462008700095	39.0	35.0	39.0	32.0	39.0
12	36.6080757515961	39.0	35.0	39.0	32.0	39.0
13	36.658559506117825	39.0	35.0	39.0	32.0	39.0
14	37.68741042311447	39.0	37.0	41.0	32.0	41.0
15	37.676735785245846	39.0	36.0	41.0	32.0	41.0
16	37.639298075997075	39.0	36.0	41.0	32.0	41.0
17	37.66354906059536	39.0	36.0	41.0	32.0	41.0
18	37.645786007468914	39.0	36.0	41.0	32.0	41.0
19	37.71071473306481	40.0	37.0	41.0	32.0	41.0
20	37.67183120488897	39.0	37.0	41.0	32.0	41.0
21	37.62165115416886	39.0	37.0	41.0	32.0	41.0
22	37.52138169179068	39.0	36.0	41.0	32.0	41.0
23	37.43234391984989	39.0	36.0	41.0	32.0	41.0
24	37.35997083184628	39.0	36.0	41.0	31.0	41.0
25	37.30214373970475	39.0	36.0	41.0	31.0	41.0
26	37.09419853353551	39.0	36.0	41.0	31.0	41.0
27	36.944210398440504	39.0	36.0	40.0	31.0	41.0
28	36.853156536807326	39.0	35.0	40.0	30.0	41.0
29	36.71546761888037	39.0	35.0	40.0	30.0	41.0
30	36.552457357153784	38.0	35.0	40.0	30.0	41.0
31	36.344338426115634	38.0	35.0	40.0	30.0	41.0
32	36.21641492296624	38.0	35.0	40.0	30.0	41.0
33	36.29805963169069	38.0	35.0	40.0	30.0	41.0
34	36.268530810042556	38.0	35.0	40.0	30.0	41.0
35	36.20951187717903	38.0	35.0	40.0	30.0	41.0
36	36.14102619783612	38.0	35.0	40.0	30.0	41.0
37	36.030118604989305	38.0	35.0	40.0	29.0	41.0
38	35.9317843495294	38.0	35.0	40.0	29.0	41.0
39	35.89539622803026	38.0	35.0	40.0	28.0	41.0
40	35.76093884444008	38.0	35.0	40.0	27.0	41.0
41	35.65455879862959	38.0	35.0	40.0	27.0	41.0
42	35.53216396354547	38.0	35.0	40.0	26.0	41.0
43	35.20085804978728	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	4.0
15	7.0
16	33.0
17	77.0
18	164.0
19	397.0
20	817.0
21	1770.0
22	3198.0
23	5597.0
24	9371.0
25	14837.0
26	22992.0
27	34021.0
28	48278.0
29	65717.0
30	85899.0
31	107469.0
32	133132.0
33	163848.0
34	202751.0
35	248972.0
36	316418.0
37	425200.0
38	576150.0
39	710315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.277060323442974	22.259915063645614	13.338073000952338	23.12495161195907
2	17.906564974674566	23.029623237337756	36.161429428363355	22.902382359624326
3	19.51273321462134	26.239693740680387	31.648642711517894	22.59893033318038
4	12.895326360419936	16.34474063695342	36.48206511031844	34.27786789230821
5	12.106262970355361	39.580599212321374	34.77971875454375	13.533419062779508
6	31.115382896534882	37.18892390661911	16.180614696494473	15.515078500351542
7	25.35130504513385	34.07468532824244	22.40433959687018	18.169670029753533
8	26.897771097343202	33.944926698805766	20.717005335745338	18.440296868105687
9	25.724656153794346	13.938651202635583	22.83928120706053	37.497411436509545
10	16.185901975113286	27.338125873738527	34.232170698531334	22.243801452616857
11	32.42924016141306	22.615358663174547	24.250606935524786	20.704794239887608
12	20.205649960754545	27.329534045982957	31.71347481839142	20.751341174871072
13	30.03816282174507	21.17961074299483	27.905973303019604	20.876253132240503
14	21.033801446322478	22.479274182533224	28.06226274123996	28.424661629904346
15	23.26493860777142	29.171143543949558	25.74401137016678	21.81990647811224
16	22.167702406781817	28.332763692005948	26.769869309802424	22.729664591409808
17	20.286406847277586	27.46457995403844	27.842998038041973	24.406015160642003
18	21.14842209352315	26.551706122983358	30.862097073176564	21.43777471031693
19	20.82715697363725	25.8085287580749	31.60697392049821	21.757340347789633
20	23.702523857271174	25.052353499895197	30.74001758649579	20.50510505633784
21	23.479797245453724	25.6850330360498	29.822013836304595	21.013155882191878
22	22.42032732031278	25.300257628945083	30.677671759448966	21.601743291293175
23	21.59450475508885	26.40202578303652	31.250932354935014	20.752537106939617
24	20.64509205215019	26.13045478778815	30.37019133024783	22.854261829813833
25	21.693483869708867	26.53313770402444	30.6970269758214	21.076351450445298
26	22.26051302967989	26.19730109604027	30.032686711747008	21.509499162532833
27	21.491874900470126	27.011258756268415	29.783523706835506	21.71334263642595
28	21.264742223137006	26.550258415742494	30.068722033285937	22.116277327834563
29	20.560936200800768	26.55642690746444	30.754211411835573	22.128425479899214
30	20.37578703345274	26.931918105089697	31.555832088619823	21.13646277283774
31	21.26955742330771	26.530210817646164	30.804346143024663	21.395885616021463
32	21.706041156428544	26.506512479551137	29.7184712966862	22.068975067334122
33	22.080493781467965	26.405456219759444	29.700941450313113	21.813108548459482
34	21.330140824148263	27.86395832113797	29.768448668392583	21.037452186321183
35	20.683896900584685	28.413552050425533	29.89320326627931	21.00934778271047
36	21.08513210958011	26.504183559207135	30.869178249898187	21.54150608131457
37	21.212372987293538	26.413355665791116	30.434519886776705	21.93975146013864
38	21.12094712784325	26.119596983481657	30.96019497469345	21.79926091398164
39	21.689518410744757	25.76796148343414	31.067293838620923	21.475226267200178
40	20.642416940944244	26.217065447067732	30.706500016680106	22.434017595307918
41	21.138791693181737	25.910182983900864	30.71773548374508	22.233289839172315
42	19.69838593231402	26.93487646336451	31.03465748190838	22.33208012241309
43	19.910600930686925	26.581793256076118	30.717294877193513	22.790310936043443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	327.0
1	436.0
2	545.0
3	1510.5
4	2476.0
5	2476.0
6	3961.0
7	5446.0
8	5668.5
9	5891.0
10	8756.0
11	11621.0
12	11621.0
13	20091.5
14	28562.0
15	45448.5
16	62335.0
17	62104.5
18	61874.0
19	61874.0
20	67953.5
21	74033.0
22	64151.5
23	54270.0
24	61282.0
25	68294.0
26	68294.0
27	75638.0
28	82982.0
29	89627.5
30	96273.0
31	103846.0
32	111419.0
33	111419.0
34	120995.5
35	130572.0
36	141154.0
37	151736.0
38	159547.5
39	167359.0
40	167359.0
41	174432.0
42	181505.0
43	195744.0
44	209983.0
45	240925.5
46	271868.0
47	271868.0
48	274511.0
49	277154.0
50	256907.0
51	236660.0
52	226536.0
53	216412.0
54	216412.0
55	194780.5
56	173149.0
57	151953.0
58	130757.0
59	117602.5
60	104448.0
61	104448.0
62	92476.0
63	80504.0
64	70500.0
65	60496.0
66	51776.0
67	43056.0
68	43056.0
69	36257.5
70	29459.0
71	24868.0
72	20277.0
73	15770.5
74	11264.0
75	11264.0
76	8689.0
77	6114.0
78	4810.0
79	3506.0
80	2801.5
81	2097.0
82	2097.0
83	1579.5
84	1062.0
85	910.5
86	759.0
87	622.0
88	485.0
89	485.0
90	399.5
91	314.0
92	184.0
93	54.0
94	44.0
95	34.0
96	34.0
97	19.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3177438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.93361774773599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90454597688229	35.15541328446709
2	13.597335794150558	12.76344321100541
3	4.9330416141783955	6.945764683605699
4	2.167354177938163	4.068870900452332
5	1.202425303279742	2.8217084777168466
6	0.7014953887676861	1.9754229856933214
7	0.4421002407327168	1.452455459331197
8	0.31372621300530484	1.1779444926908622
9	0.21084657717337804	0.8906213390826456
>10	1.3036952843422573	11.604710234696727
>50	0.1299534966653352	4.207521268988892
>100	0.08286096378987683	7.290828163145453
>500	0.006115988529220176	1.9048778850027845
>1k	0.0037636852485073732	3.2207447786567425
>5k	4.7046065606342165E-4	1.4727325103638866
>10k+	2.6883466060766955E-4	3.046940325100123
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29734	0.9357853717366005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28555	0.8986800057152964	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25934	0.8161921648825248	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12372	0.38937030399963746	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9940	0.31283065161302914	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7027	0.22115301699041806	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6658	0.2095398871669565	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6404	0.2015460254456578	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6135	0.19308008527625087	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5377	0.16922438769851686	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5148	0.16201732339073177	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4610	0.14508544305191792	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4379	0.13781543495105178	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3931	0.12371602530088707	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3915	0.12321247495623833	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3762	0.11839727478553477	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3447	0.10848362737526272	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3268	0.10285015789450495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.1471896540546186E-5	0.0	0.0	0.0	0.0
7	3.1471896540546186E-5	0.0	0.0	0.0	0.0
8	3.1471896540546186E-5	0.0	0.0	0.0	0.0
9	3.1471896540546186E-5	0.0	0.0	6.294379308109237E-5	0.0
10	3.1471896540546186E-5	0.0	0.0	9.441568962163856E-5	0.0
11	3.1471896540546186E-5	0.0	0.0	3.461908619460081E-4	0.0
12	3.1471896540546186E-5	0.0	0.0	5.979660342703776E-4	0.0
13	3.1471896540546186E-5	0.0	0.0	7.238536204325623E-4	0.0
14	3.1471896540546186E-5	0.0	0.0	8.812131031352933E-4	0.0
15	3.1471896540546186E-5	0.0	0.0	0.0011329882754596628	0.0
16	3.1471896540546186E-5	0.0	0.0	0.001825369999351679	0.0
17	3.1471896540546186E-5	0.0	0.0	0.0027065831024869723	0.0
18	3.1471896540546186E-5	0.0	0.0	0.0030527739644329805	0.0
19	3.1471896540546186E-5	0.0	0.0	0.003650739998703358	0.0
20	3.1471896540546186E-5	0.0	0.0	0.004563424998379197	0.0
21	3.1471896540546186E-5	0.0	0.0	0.00635732310119033	0.0
22	3.1471896540546186E-5	0.0	0.0	0.010291310168758604	0.0
23	3.1471896540546186E-5	0.0	0.0	0.016365386201084017	0.0
24	3.1471896540546186E-5	0.0	0.0	0.026027258439031697	0.0
25	3.1471896540546186E-5	0.0	0.0	0.031408952747465094	0.0
26	3.1471896540546186E-5	0.0	0.0	0.0396545896410882	0.0
27	3.1471896540546186E-5	0.0	0.0	0.10215777617061293	0.0
28	3.1471896540546186E-5	0.0	0.0	0.16991676942240888	0.0
29	3.1471896540546186E-5	0.0	0.0	0.2544188116337754	0.0
30	3.1471896540546186E-5	0.0	0.0	0.39645148072126035	0.0
31	3.1471896540546186E-5	0.0	0.0	0.6284308301216263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7150	0.0	28.125175	1
ATTGGAC	1030	0.0	18.679613	3
GTATTGG	1160	0.0	17.224138	1
TGGACCC	1550	0.0	16.351612	5
GTATCAA	12420	0.0	16.221016	2
GGACCCT	1535	0.0	16.029316	6
TTGGACC	1595	0.0	15.890283	4
CGAACGA	250	0.0	14.800001	16
ACGAACG	265	0.0	14.660377	15
CGTCCCG	90	8.2796824E-4	14.388888	32
GTCGGGT	180	3.3378456E-9	14.388888	24
TATTGGA	1500	0.0	14.060001	2
CGTCTAT	145	8.9234527E-7	14.034483	1
TAGGTCG	185	4.9076334E-9	14.0	21
GATAACG	265	0.0	13.962263	11
AACGGTA	120	3.303963E-5	13.875001	18
GACCCTC	1795	0.0	13.810584	7
ATAACGA	275	0.0	13.454545	12
GTCTATA	265	1.8189894E-12	13.26415	1
ACCCTCG	1845	0.0	13.135502	8
>>END_MODULE