FastQCFastQC Report
Wed 25 May 2016
SRR2088392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307706
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169701.2976922947512668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159571.2202283999614592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136971.0474066800947615No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG71190.5443884175800983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.43526603074391335No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT43370.3316494686114463No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT41680.3187260745152198No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41050.3139084778994667No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39830.3045791638181671No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34180.26137373385149265No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT31670.24217981717603193No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG28060.21457422386989125No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT25520.19515089783177564No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA23220.17756284669489933No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22650.17320406880445605No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.16158066109660732No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA19770.1511807699895848No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT18810.14383967038462775No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17520.13397506779046667No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15270.11676936559134853No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14910.11401645323948961No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC14890.11386351366438634No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.11225764812580197No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14290.10927532641128816No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13990.10698123278473907No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13710.10484007873329326No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.10185775701877944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG250.00549661729.634
GGTATCA39600.028.5441931
GACGTAT451.323348E-424.66666828
TTATGCG603.7267913E-521.5833344
TAATACG555.143616E-420.1818184
GCGCATT656.9030604E-519.92307711
GTATTGG4300.018.9302331
GGACGTA609.2377426E-418.527
CGTGTCT801.6169482E-518.535
ATAATAC1800.017.4722233
GTATCAA65350.017.3534812
ATTGGAC4400.017.2386363
TATGCGG650.001580091517.0769235
ACGTATA650.001580091517.07692329
TTGGACC7050.017.0567384
ACGGACC1201.04149876E-716.9583348
AAGACGG1452.9795046E-916.5862065
ATTAGAC1403.473906E-815.8571433
TGGACGT700.002592749215.85714326
GTAGACT700.002592749215.8571434
Produced by FastQC (version 0.11.5)