##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1307706
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092728793780864	31.0	31.0	33.0	30.0	34.0
2	31.321977569881916	31.0	31.0	34.0	28.0	34.0
3	31.387278944961636	31.0	31.0	34.0	28.0	34.0
4	35.21800695263308	37.0	35.0	37.0	33.0	37.0
5	35.03016274300187	35.0	35.0	37.0	32.0	37.0
6	35.09748062637932	37.0	35.0	37.0	32.0	37.0
7	34.96047276681456	37.0	35.0	37.0	32.0	37.0
8	35.00282555865003	37.0	35.0	37.0	32.0	37.0
9	36.43781706285664	38.0	35.0	39.0	32.0	39.0
10	36.29927980754084	38.0	35.0	39.0	32.0	39.0
11	36.434193924322436	38.0	35.0	39.0	32.0	39.0
12	36.274798005056184	38.0	35.0	39.0	32.0	39.0
13	36.33341745009964	38.0	35.0	39.0	32.0	39.0
14	37.259143110148614	39.0	36.0	41.0	32.0	41.0
15	37.24986808961647	39.0	36.0	41.0	32.0	41.0
16	37.18261367616269	39.0	36.0	41.0	31.0	41.0
17	37.250460730469996	39.0	36.0	41.0	32.0	41.0
18	37.23165145682592	39.0	36.0	40.0	31.0	41.0
19	37.315183229257954	39.0	36.0	41.0	31.0	41.0
20	37.27060822539623	39.0	36.0	40.0	31.0	41.0
21	37.215590507346455	39.0	36.0	41.0	31.0	41.0
22	37.110775663643054	39.0	36.0	40.0	31.0	41.0
23	36.99178867421271	39.0	36.0	40.0	31.0	41.0
24	36.923683152023465	39.0	36.0	40.0	31.0	41.0
25	36.857087143440495	39.0	35.0	40.0	30.0	41.0
26	36.59396225145407	38.0	35.0	40.0	30.0	41.0
27	36.42996055688358	38.0	35.0	40.0	30.0	41.0
28	36.314353532063016	38.0	35.0	40.0	30.0	41.0
29	36.15001613512517	38.0	35.0	40.0	30.0	41.0
30	35.95350713386648	38.0	35.0	40.0	29.0	41.0
31	35.6953627191433	38.0	34.0	40.0	28.0	41.0
32	35.55496648329212	38.0	34.0	40.0	27.0	41.0
33	35.61145242126288	38.0	34.0	40.0	28.0	41.0
34	35.55950114169393	38.0	34.0	40.0	27.0	41.0
35	35.48563209161692	38.0	34.0	40.0	27.0	41.0
36	35.39730795759903	38.0	34.0	40.0	27.0	41.0
37	35.263686180227054	38.0	34.0	40.0	26.0	41.0
38	35.144839130507926	38.0	34.0	40.0	26.0	41.0
39	35.07850541329626	38.0	34.0	40.0	25.0	41.0
40	34.89838541690563	38.0	33.0	40.0	24.0	41.0
41	34.74534719577642	38.0	33.0	40.0	24.0	41.0
42	34.573482877649866	38.0	33.0	40.0	23.0	41.0
43	34.21435322618387	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	4.0
15	9.0
16	12.0
17	50.0
18	117.0
19	297.0
20	521.0
21	1096.0
22	1965.0
23	3400.0
24	5492.0
25	8586.0
26	13027.0
27	18437.0
28	25404.0
29	33644.0
30	42863.0
31	52015.0
32	62256.0
33	75343.0
34	91036.0
35	109458.0
36	138305.0
37	183457.0
38	234504.0
39	206407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.809325643531494	22.853531298319346	13.982883002754443	22.354260055394715
2	18.81852648836971	23.376966994110298	35.16654355030871	22.637962967211283
3	20.630248695043075	25.895346507548332	31.853337065058966	21.621067732349626
4	13.820767053144973	16.454004187485566	34.85577033369886	34.8694584256706
5	11.96385120202859	39.992016554179614	34.228335726837685	13.815796516954116
6	31.829325551767752	36.352513485447034	15.208464287844514	16.609696674940697
7	25.019385091144343	34.77555352655719	21.55346844015398	18.651592942144486
8	27.769544530651384	33.5490546040165	20.120348151648766	18.561052713683353
9	25.54977953760249	13.506170347157543	22.970835952423556	37.97321416281642
10	16.656496184922297	27.422218755591853	33.53506063289455	22.386224426591298
11	32.329514432143	21.690119950508755	24.361744918200266	21.61862069914797
12	20.351286910054707	27.280979057983977	31.388094877594813	20.9796391543665
13	30.995116639366955	20.628413420141836	28.307433016289597	20.069036924201615
14	21.25569508742791	22.208049821595985	28.09324114135746	28.443013949618646
15	23.580376628997648	28.672040963335792	26.243589920058486	21.50399248760807
16	22.215696800351147	27.824679247476112	26.952770729812357	23.006853222360377
17	20.095801349844688	26.877906807799306	27.84777312331671	25.178518719039296
18	21.09556735229478	25.548632490789213	31.77403789536792	21.581762261548086
19	20.57809629993286	24.524319686535048	32.948002073860636	21.949581939671457
20	24.32542176911324	23.605994007827448	31.801031730373648	20.267552492685663
21	24.126906200629193	24.381244714025936	30.61024419861957	20.881604886725302
22	22.426906353568768	24.104194673726358	31.595022122709537	21.873876849995337
23	21.44595191885638	25.635578639235423	32.64265821216695	20.27581122974124
24	20.421256765664452	24.86376907347676	31.204185038533126	23.510789122325658
25	21.497263146303528	25.682836967942336	31.974618148115862	20.845281737638278
26	22.54398159831032	25.121395787738223	30.769913115027386	21.56470949892407
27	21.60393849993806	26.282818921072476	30.37525254147339	21.737990037516077
28	21.385081967965277	25.702336763768002	30.707819647535455	22.204761620731265
29	20.495126580439337	25.569049924065503	31.596245639310368	22.3395778561848
30	20.00464936308314	25.910793404633765	32.90709073752051	21.177466494762584
31	21.128449360941985	25.359675645749117	32.055752592708146	21.456122400600748
32	21.904694174378644	25.637719793286873	30.367834972080882	22.089751060253604
33	22.6480569791681	25.49778008206738	30.144619662217654	21.70954327654687
34	21.535727449442	27.80020891545959	30.03266789324206	20.631395741856352
35	20.41085687455743	28.658811690089365	30.263300772497793	20.667030662855414
36	21.07117349006581	25.64521383246693	31.836360772222505	21.44725190524476
37	21.183048789253853	25.787524107100523	31.216267264966284	21.813159838679336
38	21.294924088441896	25.408081021269307	31.787267168614353	21.509727721674444
39	22.182203033403532	25.164907096855103	31.475729254128986	21.17716061561238
40	20.83893474527149	25.768636069575273	30.760583800946083	22.631845384207153
41	21.41100522594528	25.48164495689398	30.748960393238235	22.358389423922503
42	19.513636857214085	26.88922433635695	31.09200386019488	22.505134946234094
43	20.058178214369285	26.588545131703917	30.74314868938431	22.61012796454249
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	136.0
2	178.0
3	530.0
4	882.0
5	882.0
6	1607.5
7	2333.0
8	2506.5
9	2680.0
10	4093.5
11	5507.0
12	5507.0
13	10033.5
14	14560.0
15	23854.0
16	33148.0
17	31799.5
18	30451.0
19	30451.0
20	33274.5
21	36098.0
22	28826.5
23	21555.0
24	23258.5
25	24962.0
26	24962.0
27	27344.0
28	29726.0
29	30929.0
30	32132.0
31	34503.5
32	36875.0
33	36875.0
34	40307.0
35	43739.0
36	48384.0
37	53029.0
38	56267.5
39	59506.0
40	59506.0
41	63126.5
42	66747.0
43	75439.5
44	84132.0
45	103109.5
46	122087.0
47	122087.0
48	124401.0
49	126715.0
50	116954.0
51	107193.0
52	101606.5
53	96020.0
54	96020.0
55	84655.0
56	73290.0
57	63052.5
58	52815.0
59	47453.0
60	42091.0
61	42091.0
62	37644.5
63	33198.0
64	29261.5
65	25325.0
66	21683.0
67	18041.0
68	18041.0
69	15230.0
70	12419.0
71	10578.0
72	8737.0
73	6854.0
74	4971.0
75	4971.0
76	3836.5
77	2702.0
78	2153.0
79	1604.0
80	1265.0
81	926.0
82	926.0
83	699.0
84	472.0
85	404.0
86	336.0
87	282.0
88	228.0
89	228.0
90	187.5
91	147.0
92	93.5
93	40.0
94	26.0
95	12.0
96	12.0
97	6.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1307706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.52125282497891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01548976627633	39.62467298630283
2	11.21704723248656	11.109644638993938
3	3.494472845644732	5.191520198375888
4	1.5143527942612878	2.99970590363306
5	0.8474143742960082	2.098251073851697
6	0.535635024862737	1.5915190492884879
7	0.37116307901123646	1.2866322472509142
8	0.2691564917282198	1.0663173341086014
9	0.2079066937599389	0.9266219951122259
>10	1.3248556983444535	12.399195059191454
>50	0.11951757089953371	4.085954281676094
>100	0.07150966230384305	6.307260582655045
>500	0.006514975368331211	2.319400581173273
>1k	0.004188198451070065	4.427709130914336
>5k	3.1023692230148624E-4	0.9840975308144267
>10k+	4.6535538345222937E-4	3.5814974066577028
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16970	1.2976922947512668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15957	1.2202283999614592	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13697	1.0474066800947615	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7119	0.5443884175800983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5692	0.43526603074391335	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4337	0.3316494686114463	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4168	0.3187260745152198	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4105	0.3139084778994667	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3983	0.3045791638181671	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3418	0.26137373385149265	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3167	0.24217981717603193	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2806	0.21457422386989125	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2552	0.19515089783177564	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2322	0.17756284669489933	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2265	0.17320406880445605	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2113	0.16158066109660732	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1977	0.1511807699895848	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1881	0.14383967038462775	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1752	0.13397506779046667	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1527	0.11676936559134853	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1491	0.11401645323948961	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1489	0.11386351366438634	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1468	0.11225764812580197	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1429	0.10927532641128816	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1399	0.10698123278473907	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1371	0.10484007873329326	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1332	0.10185775701877944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.646978755163623E-5	0.0
3	0.0	0.0	0.0	7.646978755163623E-5	0.0
4	0.0	0.0	0.0	7.646978755163623E-5	0.0
5	0.0	0.0	0.0	7.646978755163623E-5	0.0
6	7.646978755163623E-5	0.0	0.0	7.646978755163623E-5	0.0
7	7.646978755163623E-5	0.0	0.0	7.646978755163623E-5	0.0
8	7.646978755163623E-5	0.0	0.0	7.646978755163623E-5	0.0
9	7.646978755163623E-5	0.0	0.0	1.5293957510327245E-4	0.0
10	7.646978755163623E-5	0.0	0.0	3.058791502065449E-4	0.0
11	7.646978755163623E-5	0.0	0.0	6.117583004130898E-4	0.0
12	7.646978755163623E-5	0.0	0.0	0.0011470468132745433	0.0
13	7.646978755163623E-5	0.0	0.0	0.0012235166008261796	0.0
14	7.646978755163623E-5	0.0	0.0	0.0016058655385843606	0.0
15	7.646978755163623E-5	0.0	0.0	0.001758805113687633	0.0
16	7.646978755163623E-5	0.0	0.0	0.0029823217145138127	0.0
17	7.646978755163623E-5	0.0	0.0	0.004511717465546537	0.0
18	7.646978755163623E-5	0.0	0.0	0.005047005978407991	0.0
19	7.646978755163623E-5	0.0	0.0	0.005964643429027625	0.0
20	7.646978755163623E-5	0.0	0.0	0.007723448542715259	0.0
21	7.646978755163623E-5	0.0	0.0	0.011470468132745434	0.0
22	7.646978755163623E-5	0.0	0.0	0.02064684263894178	0.0
23	7.646978755163623E-5	0.0	0.0	0.03563492099906248	0.0
24	7.646978755163623E-5	0.0	0.0	0.057658219813933716	0.0
25	7.646978755163623E-5	0.0	0.0	0.06530519856909733	0.0
26	7.646978755163623E-5	0.0	0.0	0.07555215010101658	0.0
27	7.646978755163623E-5	0.0	0.0	0.1317574439514692	0.0
28	7.646978755163623E-5	0.0	0.0	0.19675676337036	0.0
29	7.646978755163623E-5	0.0	0.0	0.27047363857013734	0.0
30	7.646978755163623E-5	0.0	0.0	0.41286038299128397	0.0
31	7.646978755163623E-5	0.0	0.0	0.6144347429773971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	25	0.005496617	29.6	34
GGTATCA	3960	0.0	28.544193	1
GACGTAT	45	1.323348E-4	24.666668	28
TTATGCG	60	3.7267913E-5	21.583334	4
TAATACG	55	5.143616E-4	20.181818	4
GCGCATT	65	6.9030604E-5	19.923077	11
GTATTGG	430	0.0	18.930233	1
GGACGTA	60	9.2377426E-4	18.5	27
CGTGTCT	80	1.6169482E-5	18.5	35
ATAATAC	180	0.0	17.472223	3
GTATCAA	6535	0.0	17.353481	2
ATTGGAC	440	0.0	17.238636	3
TATGCGG	65	0.0015800915	17.076923	5
ACGTATA	65	0.0015800915	17.076923	29
TTGGACC	705	0.0	17.056738	4
ACGGACC	120	1.04149876E-7	16.958334	8
AAGACGG	145	2.9795046E-9	16.586206	5
ATTAGAC	140	3.473906E-8	15.857143	3
TGGACGT	70	0.0025927492	15.857143	26
GTAGACT	70	0.0025927492	15.857143	4
>>END_MODULE