FastQCFastQC Report
Wed 25 May 2016
SRR2088391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3951080
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377390.9551565647873492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363470.9199256912034178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT335470.8490589914656246No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG184800.4677202182694352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127320.32224100752199397No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102970.2606122882857345No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91480.23153163185761866No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT89960.22768458244328132No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT89560.22667220101845573No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA74190.18777144476953136No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT71820.1817730848274396No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67110.16985229355011794No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT63040.15955131255251728No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57820.14633973495854297No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA53970.13659556374459642No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47690.12070117537483421No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.11667695921115238No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT45310.11467750589712179No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39620.1002763801289774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86400.029.0133081
CGCGATA802.7301212E-823.12514
GTATTGG12800.021.3906251
ATTGGAC13250.019.9660383
AATGCGT3150.018.2063535
GGACCCT20650.017.0217916
GTATCAA151450.016.5638832
TTGGACC21850.016.5102984
TGGACCC21500.016.3488395
GAACCGT1403.4799086E-815.8571436
TATTGGA19850.015.8438292
GACCCTC21000.015.3285717
ACCGTGT1853.0559022E-1015.08
CGTCTGT7100.014.59154934
CGTTTAG3450.014.47826126
ACGGACC5300.014.3113218
TACCGTC4400.014.2954547
ATAATAC6050.014.0661153
GACCGTG4900.013.9693877
ACTATCG1203.304365E-513.8749998
Produced by FastQC (version 0.11.5)