FastQCFastQC Report
Wed 25 May 2016
SRR2088390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687092
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT364910.9896959446631654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364540.9886924438012396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323150.8764359554901261No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG149340.40503464518921684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128790.3492996648849554No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA81970.2223161233839568No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT78580.21312188575712243No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT78100.2118200468011105No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70310.19069228541083325No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA65190.17680600321337248No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62760.170215443498562No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59500.16137378725564755No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG56050.15201681975931167No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49650.1346589670124857No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47390.1285294752612628No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.12413576878472249No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA43120.11694853288173987No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT42970.11654170820798614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88750.026.0771831
CGCACTA1107.4578566E-1121.86363629
ATTGGAC9500.018.1105273
GTATTGG10000.017.9451
GGACCCT15150.016.2409256
GTATCAA152750.015.2117842
TTGGACC16850.015.0415444
TGGACCC16900.014.9970425
CGAATTA2600.014.94230715
CGTCTGT9050.014.30939234
TATTGGA13750.014.2618182
TATACCG2750.014.1272725
GACCCTC16900.014.0118357
ACGTTTA2304.0017767E-1113.67391326
TTATGCG1907.1413524E-913.6315794
CGTACGA2051.4260877E-913.53658512
ACCGTGG3150.013.5079378
GACCGTG3500.013.2142867
AAGACCG2254.0745363E-1013.1555565
CGATGCG3100.013.12903233
Produced by FastQC (version 0.11.5)