FastQCFastQC Report
Wed 25 May 2016
SRR2088389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452518
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191281.316885573879291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181961.252721136674382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160691.1062857740833505No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78900.5431946454364077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60680.41775730145857054No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT45930.3162095065259088No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA45910.3160718146005764No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT44450.30602030405130953No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39760.2737315475608564No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34050.2344205028784497No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT33950.2337320432517876No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG32870.22629667928383673No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT30520.21011787805727708No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26620.18326795261745465No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25810.17769142964149154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.16819068679355437No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23840.1641287749962479No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT21660.14912035513501382No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20510.14120306942839952No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.11807082597255249No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC16680.11483506572724056No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16290.11215007318325831No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15440.10629816635663034No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14950.10292471418598599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.029.1647051
CAATGCG509.093248E-625.89999819
TGCGACG551.9031071E-523.54545422
TAACCGC400.001931431123.1256
GTATTGG4350.022.9655151
ATTGGAC4500.021.3777773
TTGGACC8150.019.2944774
CTCGACG609.2384143E-418.518
TGGACGA500.00703575618.49999810
GTCGTCA1002.8762224E-718.49999824
GGACCCT8750.017.766
AGGTCGT953.6077108E-617.52631622
TATTGGA6350.017.4803142
ACCGACC752.0677397E-417.2666668
CGTCTGT3650.017.23287834
GTATCAA72000.017.2152792
TGGACCC9350.016.620325
GACCCTC8950.016.5363147
TAGAACC1251.6584818E-716.2799994
GCTGCGC1151.2426699E-616.0869568
Produced by FastQC (version 0.11.5)