FastQCFastQC Report
Wed 25 May 2016
SRR2088388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997948
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65500.6563468236821959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.6382096061117413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54140.5425132371626578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.2246610043809898No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG15690.15722262081791838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAT451.3230415E-424.66666610
GGTATCA19800.023.1717151
AGTGCGC555.142442E-420.1818188
ATACGGG656.900968E-519.9230773
GACCGTC759.263766E-619.73333422
TACGGGC759.263766E-619.7333344
GGGCGTC951.6755621E-719.4736847
AACCCGC902.151637E-618.56
ACCGACC902.151637E-618.58
ATGCGAC500.007034247718.521
TATACCG701.2189927E-418.55
GTGCGAA500.007034247718.512
CTAGCGG701.2189927E-418.529
AACCCGT953.6054244E-617.52631629
CGTTGAA953.6054244E-617.52631633
CCCGTTG953.6054244E-617.52631631
CGTAATG852.7225327E-517.41176421
TACCGAC852.7225327E-517.4117647
ACGTTTA752.0669225E-417.26666626
GGTCTAG650.001579737217.0769231
Produced by FastQC (version 0.11.5)