FastQCFastQC Report
Wed 25 May 2016
SRR2088387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497589
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133220.8895631578490495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT128560.8584464763029108No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118970.7944102153528105No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52170.3483599305283359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.28812978727808497No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT27460.18336138954012082No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT25750.17194303644057213No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25430.16980626860907766No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25200.16827046673019097No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA21640.1444989246048148No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT21220.1416944168259783No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT20490.13681991521038148No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG18920.12633639803711166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17140.11445062697442357No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA16510.1102438653061688No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT15510.10356646583274852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.027.8823531
GTATTGG3500.018.51
TAGGTCG500.007035854718.49999821
CGCATAC500.007035854718.49999837
TAATGCG500.007035854718.4999985
CGGGATA1156.4086635E-817.69565224
ATTGGAC3850.017.2987023
GCGTTAT1059.346795E-615.8571441
TTGGACC5600.015.8571424
GTATCAA55450.015.7141582
TAGACTG1902.7284841E-1115.5789485
TCTAGAC1455.3483745E-815.3103453
GACCCTC5350.015.2149527
TAATACA4050.015.0740744
ACAACCG750.004105177214.829
CTAACGC750.004105177214.83
GCGCTAG750.004105177214.811
TCGATCC1001.0937154E-414.79999932
CGAATTA1001.0937154E-414.79999915
CGGTATA1152.2106175E-514.47826126
Produced by FastQC (version 0.11.5)