##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1497589
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36472823985753	31.0	31.0	34.0	30.0	34.0
2	31.613060058534085	31.0	31.0	34.0	30.0	34.0
3	31.705684269849737	31.0	31.0	34.0	30.0	34.0
4	35.49308788993509	37.0	35.0	37.0	33.0	37.0
5	35.2932500171943	37.0	35.0	37.0	33.0	37.0
6	35.33536838211285	37.0	35.0	37.0	32.0	37.0
7	35.20969705306329	37.0	35.0	37.0	32.0	37.0
8	35.22397066217767	37.0	35.0	37.0	32.0	37.0
9	36.750676587501644	39.0	37.0	39.0	32.0	39.0
10	36.61089457788486	39.0	35.0	39.0	32.0	39.0
11	36.72573449724858	39.0	35.0	39.0	32.0	39.0
12	36.58292428697059	39.0	35.0	39.0	32.0	39.0
13	36.62840071608432	39.0	35.0	39.0	32.0	39.0
14	37.65853581990787	39.0	36.0	41.0	32.0	41.0
15	37.64875743611899	39.0	36.0	41.0	32.0	41.0
16	37.61367905346527	39.0	36.0	41.0	32.0	41.0
17	37.64369730279803	39.0	36.0	41.0	32.0	41.0
18	37.6105820755895	39.0	36.0	41.0	32.0	41.0
19	37.67325013738749	39.0	37.0	41.0	32.0	41.0
20	37.6353906178531	39.0	37.0	41.0	32.0	41.0
21	37.57734264875076	39.0	36.0	41.0	32.0	41.0
22	37.47626017552212	39.0	36.0	41.0	32.0	41.0
23	37.36436832802591	39.0	36.0	41.0	31.0	41.0
24	37.298220673362316	39.0	36.0	41.0	31.0	41.0
25	37.237993868811806	39.0	36.0	41.0	31.0	41.0
26	37.01064911668021	39.0	36.0	41.0	31.0	41.0
27	36.88005187003911	39.0	35.0	40.0	31.0	41.0
28	36.79715462653638	39.0	35.0	40.0	30.0	41.0
29	36.659222256573734	39.0	35.0	40.0	30.0	41.0
30	36.489791257815064	38.0	35.0	40.0	30.0	41.0
31	36.27355903388713	38.0	35.0	40.0	30.0	41.0
32	36.11486529348172	38.0	35.0	40.0	30.0	41.0
33	36.20372545471421	38.0	35.0	40.0	30.0	41.0
34	36.181183221831894	38.0	35.0	40.0	30.0	41.0
35	36.13669170914049	38.0	35.0	40.0	30.0	41.0
36	36.06993307242508	38.0	35.0	40.0	29.0	41.0
37	35.97183806772085	38.0	35.0	40.0	29.0	41.0
38	35.865397649154744	38.0	35.0	40.0	28.0	41.0
39	35.83243867309389	38.0	35.0	40.0	28.0	41.0
40	35.70281098485633	38.0	35.0	40.0	27.0	41.0
41	35.58188862231226	38.0	35.0	40.0	27.0	41.0
42	35.472008675277394	38.0	34.0	40.0	26.0	41.0
43	35.14534027693846	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	9.0
16	9.0
17	26.0
18	77.0
19	198.0
20	429.0
21	823.0
22	1506.0
23	2648.0
24	4437.0
25	7295.0
26	11282.0
27	16554.0
28	23457.0
29	31861.0
30	41220.0
31	51211.0
32	63131.0
33	77667.0
34	96633.0
35	118685.0
36	150091.0
37	200870.0
38	273250.0
39	324219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.574958149398796	21.51671787119163	13.069740763320242	23.838583216089326
2	18.22889991846895	22.57615407164449	35.58105728607782	23.613888723808735
3	19.709880347678833	25.653901036933362	31.225456383560505	23.410762231827288
4	13.485275332551186	16.083518241653753	35.86417902375084	34.56702740204422
5	12.577482874139701	39.0225222006839	34.63213204691007	13.767862878266332
6	32.1180243711726	36.61705581437898	15.867771464667543	15.397148349780881
7	25.90670738099706	33.46953002459286	22.21250289632202	18.41125969808806
8	27.13541565810112	33.78016264809637	20.435179478481746	18.649242215320758
9	26.05628112920167	13.70028759559532	22.133776356530397	38.10965491867261
10	16.100612384305705	27.34021149995092	34.23175517448379	22.327420941259586
11	33.072692173887496	22.188597806207177	23.926257471175337	20.812452548729993
12	20.54121658211966	26.76969448894189	31.51291843089125	21.176170498047195
13	30.344507071032172	21.139311252953917	27.218883151518874	21.29729852449504
14	21.297699168463442	21.99815837322523	27.740187728408795	28.96395472990253
15	23.44762147692057	28.80216134066156	25.465130953819777	22.2850862285981
16	22.65160868569414	27.8608483368935	26.389349814935876	23.098193162476488
17	20.805174183303965	27.102496078697158	27.491588146013356	24.600741591985518
18	21.53220943796996	26.31843583252815	30.30597847607054	21.843376253431348
19	21.308983973573522	25.545660391469223	31.159617224752584	21.985738410204668
20	23.857947674562247	24.853881806022883	30.30637912003894	20.98179139937593
21	23.54504473523777	25.4618590280778	29.458950352867173	21.534145883817253
22	22.666632834509333	25.26667864146972	30.05404019393839	22.012648330082552
23	22.0957151795319	26.16559015858156	30.538619073724497	21.20007558816204
24	21.34590999266154	25.98944036047273	29.605452497314015	23.059197149551714
25	21.988008726025633	26.26381470483557	30.345508680953188	21.40266788818561
26	22.75030064991129	25.793258363943643	29.50108474354446	21.955356242600608
27	21.894258037418812	26.578654090007337	29.356118400976506	22.17096947159735
28	21.69774217091605	26.296600736250063	29.504690539260103	22.50096655357378
29	21.13704093713295	26.34561284838497	30.178306598138743	22.339039616343335
30	20.961692426960933	26.612441731342845	30.901936379073298	21.523929462622924
31	21.721313391057226	26.293262036513354	30.319199727027907	21.66622484540151
32	22.04897338321796	26.32384452610162	29.40172503937996	22.225457051300456
33	22.672041528082808	26.16432145268161	29.153325778968732	22.010311240266855
34	21.774131620891982	27.45366051700433	29.2407329380758	21.531474924027886
35	20.97197562215	27.898108225955188	29.54595686800584	21.58395928388897
36	21.475651864430095	26.0969464919948	30.428174886434128	21.999226757140978
37	21.60546051019338	25.991376806320027	30.009702261434878	22.39346042205171
38	21.54803487472197	25.847612395657286	30.428174886434128	22.176177843186615
39	21.939731127832804	25.384935386143997	30.680380264545214	21.99495322147799
40	20.98606493503892	25.734964666540687	30.359931863815774	22.91903853460462
41	21.413552049327286	25.33245102628291	30.480993116268884	22.77300380812092
42	19.98705919982051	26.27790401772449	30.705821156538942	23.029215625916056
43	20.28874410802964	25.99431486208833	30.465501549490547	23.251439480391483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	176.5
2	246.0
3	652.5
4	1059.0
5	1059.0
6	1731.5
7	2404.0
8	2553.5
9	2703.0
10	3978.0
11	5253.0
12	5253.0
13	8997.5
14	12742.0
15	20482.5
16	28223.0
17	28159.5
18	28096.0
19	28096.0
20	30137.5
21	32179.0
22	27702.5
23	23226.0
24	26030.0
25	28834.0
26	28834.0
27	32705.5
28	36577.0
29	39177.0
30	41777.0
31	45669.5
32	49562.0
33	49562.0
34	54544.0
35	59526.0
36	64580.5
37	69635.0
38	72914.5
39	76194.0
40	76194.0
41	80138.0
42	84082.0
43	90594.5
44	97107.0
45	111157.5
46	125208.0
47	125208.0
48	127196.5
49	129185.0
50	119923.5
51	110662.0
52	105765.0
53	100868.0
54	100868.0
55	92756.0
56	84644.0
57	75901.5
58	67159.0
59	61100.0
60	55041.0
61	55041.0
62	49319.5
63	43598.0
64	38543.0
65	33488.0
66	28795.0
67	24102.0
68	24102.0
69	20376.0
70	16650.0
71	14136.0
72	11622.0
73	9282.0
74	6942.0
75	6942.0
76	5493.5
77	4045.0
78	3162.0
79	2279.0
80	1795.5
81	1312.0
82	1312.0
83	958.5
84	605.0
85	451.5
86	298.0
87	252.0
88	206.0
89	206.0
90	156.5
91	107.0
92	67.0
93	27.0
94	17.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1497589.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.03494369657639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06575435743383	45.66545791800154
2	11.703506806278062	13.350177034971358
3	3.6287958130374194	6.2090449464888415
4	1.5683137429190919	3.5779474410382957
5	0.7766379920923453	2.2147752075804528
6	0.45879542650416727	1.570042279134716
7	0.315816976712602	1.2608822439658363
8	0.23109051599319277	1.054418765478763
9	0.162721900907386	0.835275101080738
>10	0.966134446965081	10.433564139522979
>50	0.0765266540114026	3.0085917621242673
>100	0.03887915057150748	3.906860090214167
>500	0.004332833553918719	1.5997535478408802
>1k	0.002224968581742045	2.4217378044072513
>5k	1.1710360956537079E-4	0.34844331409009155
>10k+	3.5131082869611235E-4	2.543028404059849
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13322	0.8895631578490495	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12856	0.8584464763029108	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11897	0.7944102153528105	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5217	0.3483599305283359	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4315	0.28812978727808497	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2746	0.18336138954012082	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2575	0.17194303644057213	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2543	0.16980626860907766	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2520	0.16827046673019097	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2164	0.1444989246048148	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2122	0.1416944168259783	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2049	0.13681991521038148	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1892	0.12633639803711166	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1714	0.11445062697442357	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1651	0.1102438653061688	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1551	0.10356646583274852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.677399473420277E-5	0.0
11	0.0	0.0	0.0	1.3354798946840554E-4	0.0
12	0.0	0.0	0.0	1.3354798946840554E-4	0.0
13	0.0	0.0	0.0	4.0064396840521665E-4	0.0
14	0.0	0.0	0.0	5.341919578736222E-4	0.0
15	0.0	0.0	0.0	7.345139420762305E-4	0.0
16	0.0	0.0	0.0	9.348359262788389E-4	0.0
17	0.0	0.0	0.0	0.0014022538894182582	0.0
18	0.0	0.0	0.0	0.001469027884152461	0.0
19	0.0	0.0	0.0	0.002003219842026083	0.0
20	0.0	0.0	0.0	0.0026041857946339083	0.0
21	0.0	0.0	0.0	0.004073213678786369	0.0
22	0.0	0.0	0.0	0.007745783389167522	0.0
23	0.0	0.0	0.0	0.013087702967903745	0.0
24	0.0	0.0	0.0	0.023170576172768364	0.0
25	0.0	0.0	0.0	0.028512495751504587	0.0
26	0.0	0.0	0.0	0.03712634107221674	0.0
27	0.0	0.0	0.0	0.10877483742201632	0.0
28	0.0	0.0	0.0	0.18750137721364138	0.0
29	0.0	0.0	0.0	0.27510885830491544	0.0
30	0.0	0.0	0.0	0.42281293465697195	0.0
31	0.0	0.0	0.0	0.664200925621115	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3145	0.0	27.882353	1
GTATTGG	350	0.0	18.5	1
TAGGTCG	50	0.0070358547	18.499998	21
CGCATAC	50	0.0070358547	18.499998	37
TAATGCG	50	0.0070358547	18.499998	5
CGGGATA	115	6.4086635E-8	17.695652	24
ATTGGAC	385	0.0	17.298702	3
GCGTTAT	105	9.346795E-6	15.857144	1
TTGGACC	560	0.0	15.857142	4
GTATCAA	5545	0.0	15.714158	2
TAGACTG	190	2.7284841E-11	15.578948	5
TCTAGAC	145	5.3483745E-8	15.310345	3
GACCCTC	535	0.0	15.214952	7
TAATACA	405	0.0	15.074074	4
ACAACCG	75	0.0041051772	14.8	29
CTAACGC	75	0.0041051772	14.8	3
GCGCTAG	75	0.0041051772	14.8	11
TCGATCC	100	1.0937154E-4	14.799999	32
CGAATTA	100	1.0937154E-4	14.799999	15
CGGTATA	115	2.2106175E-5	14.478261	26
>>END_MODULE