FastQCFastQC Report
Wed 25 May 2016
SRR2088386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109972
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT245421.163143397163564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT238591.1307732993613187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217311.0299188804401196No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG115830.5489646308102667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77070.36526551063236856No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA64610.30621259429035075No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT60640.2873971787303339No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT59460.28180468745556814No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58950.27938759376901684No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA50280.23829700109764487No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT49450.2343632996077673No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT44490.21085587865620967No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG43190.20469465945519658No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37330.1769217790567837No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA35900.17014443793566927No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33380.1582011514844747No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT31990.15161338633877605No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29990.1421345875679867No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC26080.12360353597109346No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25910.12279783807557636No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC23060.10929054982720149No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG22100.1047407264172226No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21190.10042787297651343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.027.3210831
TAGTGCG652.684612E-622.769237
GGCGTAA705.1052593E-621.14285919
TATAGCG450.003826852720.5555555
GTATTGG7600.019.7171061
CGAACGA1306.9667294E-1018.516
ACGATCG500.007036782318.58
ATTGGAC7300.018.2465743
TTGGACC11850.016.860764
TGGACCC11900.016.7899175
GGACCCT12300.016.5447166
GTATCAA99000.015.753032
AGTGCGC957.064149E-515.5789488
TATTGGA9700.015.4484552
ACGAACG1455.3520125E-815.31034515
CGTCGCA1001.09405155E-414.811
AACACCG750.004105917614.85
GTACCGT908.278057E-414.3888886
GCGCTAG908.278057E-414.38888811
CGTAACG1051.6566658E-414.09523921
Produced by FastQC (version 0.11.5)