##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109972
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42020273254811	31.0	31.0	34.0	30.0	34.0
2	31.656250888637384	31.0	31.0	34.0	30.0	34.0
3	31.738487998892875	31.0	31.0	34.0	30.0	34.0
4	35.51956281884309	37.0	35.0	37.0	33.0	37.0
5	35.345975207253936	37.0	35.0	37.0	33.0	37.0
6	35.394675379578494	37.0	35.0	37.0	33.0	37.0
7	35.267635779052995	37.0	35.0	37.0	32.0	37.0
8	35.29384939705361	37.0	35.0	37.0	32.0	37.0
9	36.812004140339305	39.0	37.0	39.0	32.0	39.0
10	36.69019731067521	39.0	35.0	39.0	32.0	39.0
11	36.80516613490605	39.0	37.0	39.0	32.0	39.0
12	36.67141222727126	39.0	35.0	39.0	32.0	39.0
13	36.70863310034446	39.0	35.0	39.0	32.0	39.0
14	37.732008292053166	40.0	37.0	41.0	32.0	41.0
15	37.71962281963931	40.0	37.0	41.0	32.0	41.0
16	37.679291478749484	39.0	37.0	41.0	32.0	41.0
17	37.73818515127215	39.0	37.0	41.0	32.0	41.0
18	37.707405595903644	39.0	37.0	41.0	32.0	41.0
19	37.780522679921816	40.0	37.0	41.0	32.0	41.0
20	37.74550136210338	40.0	37.0	41.0	32.0	41.0
21	37.69614288720419	40.0	37.0	41.0	32.0	41.0
22	37.597877128227296	39.0	36.0	41.0	32.0	41.0
23	37.484672782387634	39.0	36.0	41.0	32.0	41.0
24	37.4190946609718	39.0	36.0	41.0	32.0	41.0
25	37.367251792914786	39.0	36.0	41.0	31.0	41.0
26	37.14397205270971	39.0	36.0	41.0	31.0	41.0
27	36.9953662892209	39.0	36.0	41.0	31.0	41.0
28	36.88985256676392	39.0	35.0	40.0	31.0	41.0
29	36.74349659616336	39.0	35.0	40.0	30.0	41.0
30	36.56717672082852	38.0	35.0	40.0	30.0	41.0
31	36.31524162405947	38.0	35.0	40.0	30.0	41.0
32	36.18476690685943	38.0	35.0	40.0	30.0	41.0
33	36.23287797184039	38.0	35.0	40.0	30.0	41.0
34	36.193226734762355	38.0	35.0	40.0	30.0	41.0
35	36.11286168726409	38.0	35.0	40.0	30.0	41.0
36	36.0296530001346	38.0	35.0	40.0	29.0	41.0
37	35.91984917335396	38.0	35.0	40.0	29.0	41.0
38	35.792218569725094	38.0	35.0	40.0	28.0	41.0
39	35.73027746339762	38.0	35.0	40.0	27.0	41.0
40	35.55693724845638	38.0	35.0	40.0	27.0	41.0
41	35.407821525593704	38.0	34.0	40.0	26.0	41.0
42	35.254218065453	38.0	34.0	40.0	25.0	41.0
43	34.91596049615824	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	9.0
16	14.0
17	40.0
18	118.0
19	269.0
20	547.0
21	1104.0
22	2110.0
23	3656.0
24	6311.0
25	10008.0
26	15133.0
27	22586.0
28	32044.0
29	44075.0
30	57747.0
31	70968.0
32	87234.0
33	109106.0
34	134312.0
35	165239.0
36	210479.0
37	290490.0
38	382540.0
39	463830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.284291924253026	22.245887623153294	13.67179280104191	22.798027651551774
2	17.931091028696116	22.874142405681212	36.219580165044846	22.975186400577826
3	19.56988054817789	25.969681114251753	32.315879073276804	22.144559264293555
4	12.774624497386695	15.941633348688988	36.088583166032535	35.19515898789179
5	11.83409068935512	39.81256623310641	34.68368300621999	13.669660071318482
6	31.160745261074556	36.74897107639343	15.813148231350937	16.27713543118108
7	24.965971112412866	34.816623158980306	21.768724893031756	18.448680835575068
8	27.565531675301852	33.26598646806688	20.39349337337178	18.774988483259495
9	25.601619357982003	13.679944567984789	23.306707387586187	37.41172868644703
10	16.31557196019663	27.461075312847754	33.768647166881834	22.45470556007378
11	31.986538209985728	22.09692830047034	24.79212994295659	21.12440354658735
12	20.394393859255004	27.32500715649307	31.514588819188123	20.766010165063804
13	30.647183943673184	20.93762381680894	28.354215127025384	20.060977112492488
14	21.08013755632776	22.534232681760706	28.250043128534408	28.135586633377123
15	23.159691218651243	29.033939786878687	26.352150644653104	21.454218349816966
16	22.182806217333688	28.206914594127312	26.88642313736865	22.723856051170348
17	19.944340493617922	27.055050967500993	27.76875712094758	25.23185141793351
18	20.937055088882698	26.27129649113827	31.69255326610969	21.099095153869342
19	20.363161217305255	25.161803095017376	32.750529390911346	21.724506296766023
20	24.133543004362142	24.13107851668174	31.561224509140406	20.174153969815713
21	23.670503684409084	25.052939091134856	30.488177094293196	20.788380130162864
22	22.313424064395168	24.623928658768932	31.385534973923825	21.67711230291208
23	21.33578075917595	26.103379570913738	32.298011537593865	20.26282813231645
24	20.10557486070905	25.66726003946972	30.926761113417616	23.300403986403612
25	21.255827091544344	26.268168487543907	31.88568379106453	20.59032062984722
26	22.364988729708262	25.62233053329618	30.65216031302785	21.36052042396771
27	21.24919193240479	26.783767746680997	30.346469052669896	21.62057126824432
28	20.929282473890648	26.391866811502712	30.56377051449024	22.1150802001164
29	20.049602553967542	26.411487924958244	31.30216893873473	22.236740582339483
30	19.55006985874694	26.758364565975285	32.8478292602935	20.843736314984273
31	20.867717675874374	26.18030002293869	31.80587230541448	21.146109995772456
32	21.759103912279404	26.224044679265884	30.14855173433581	21.868299674118898
33	22.353235019232482	26.200015924381937	29.910633885188997	21.536115171196585
34	21.15900116210073	28.40800731004961	29.824424210368665	20.60856731748099
35	20.11789729911108	29.139912757136116	30.191395904779778	20.55079403897303
36	20.615012900645127	26.414189382607923	31.73738798429553	21.233409732451424
37	20.945206855825575	26.4085494973393	31.014392608053566	21.63185103878156
38	20.95667620233823	25.948875150949867	31.600419342057616	21.49402930465428
39	21.826592959527424	25.704606506626625	31.46710003734647	21.001700496499478
40	20.39216634154387	26.233002144104283	30.898324717105254	22.4765067972466
41	20.980278411277496	25.81944215373474	30.969557889867733	22.23072154512003
42	19.20087091203106	27.200218770675626	31.295202021638204	22.30370829565511
43	19.553103074353594	26.784383868601104	30.955387085705404	22.707125971339902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	246.0
2	320.0
3	921.0
4	1522.0
5	1522.0
6	2638.5
7	3755.0
8	3969.5
9	4184.0
10	6339.0
11	8494.0
12	8494.0
13	14794.0
14	21094.0
15	35061.0
16	49028.0
17	48514.5
18	48001.0
19	48001.0
20	51650.0
21	55299.0
22	45784.0
23	36269.0
24	40434.5
25	44600.0
26	44600.0
27	48822.0
28	53044.0
29	55730.0
30	58416.0
31	62491.5
32	66567.0
33	66567.0
34	72187.5
35	77808.0
36	85458.5
37	93109.0
38	97138.5
39	101168.0
40	101168.0
41	106930.0
42	112692.0
43	125334.5
44	137977.0
45	167080.0
46	196183.0
47	196183.0
48	200317.5
49	204452.0
50	186840.0
51	169228.0
52	159434.5
53	149641.0
54	149641.0
55	131937.5
56	114234.0
57	97771.5
58	81309.0
59	71974.0
60	62639.0
61	62639.0
62	55811.5
63	48984.0
64	43269.0
65	37554.0
66	31951.5
67	26349.0
68	26349.0
69	22215.0
70	18081.0
71	15132.0
72	12183.0
73	9490.5
74	6798.0
75	6798.0
76	5190.0
77	3582.0
78	2894.0
79	2206.0
80	1749.0
81	1292.0
82	1292.0
83	989.0
84	686.0
85	570.5
86	455.0
87	394.5
88	334.0
89	334.0
90	274.0
91	214.0
92	121.0
93	28.0
94	20.0
95	12.0
96	12.0
97	10.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2109972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.74057446277504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28112712230053	32.9283974653462
2	13.293780187331606	11.62955164351483
3	4.789008249975976	6.284219158827545
4	2.130677863944431	3.727882950561914
5	1.1163677867564412	2.441528415223175
6	0.6853347161117543	1.798616050920659
7	0.4325518387535054	1.3244046138405579
8	0.30781563584470645	1.0771226192377463
9	0.2399942514284117	0.9447737782718157
>10	1.447017794486309	12.075928376956973
>50	0.15721602879582747	4.774667552191807
>100	0.10552529224065865	8.698663463357029
>500	0.006519951374258482	1.9242953593646743
>1k	0.0059766220930702755	4.722496967396235
>5k	6.519951374258482E-4	1.763450219285878
>10k+	4.346634249505655E-4	3.8840013657029604
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24542	1.163143397163564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23859	1.1307732993613187	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21731	1.0299188804401196	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11583	0.5489646308102667	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7707	0.36526551063236856	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6461	0.30621259429035075	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6064	0.2873971787303339	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5946	0.28180468745556814	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5895	0.27938759376901684	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5028	0.23829700109764487	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4945	0.2343632996077673	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4449	0.21085587865620967	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4319	0.20469465945519658	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3733	0.1769217790567837	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3590	0.17014443793566927	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3338	0.1582011514844747	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3199	0.15161338633877605	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2999	0.1421345875679867	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2608	0.12360353597109346	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2591	0.12279783807557636	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2306	0.10929054982720149	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2210	0.1047407264172226	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2119	0.10042787297651343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.739399385394688E-5	0.0
4	0.0	0.0	0.0	9.478798770789376E-5	0.0
5	0.0	0.0	0.0	9.478798770789376E-5	0.0
6	0.0	0.0	0.0	9.478798770789376E-5	0.0
7	0.0	0.0	0.0	9.478798770789376E-5	0.0
8	4.739399385394688E-5	0.0	0.0	9.478798770789376E-5	0.0
9	4.739399385394688E-5	0.0	0.0	9.478798770789376E-5	0.0
10	4.739399385394688E-5	4.739399385394688E-5	0.0	1.8957597541578752E-4	0.0
11	4.739399385394688E-5	4.739399385394688E-5	0.0	2.843639631236813E-4	0.0
12	4.739399385394688E-5	9.478798770789376E-5	0.0	5.213339323934156E-4	0.0
13	4.739399385394688E-5	9.478798770789376E-5	0.0	5.687279262473625E-4	0.0
14	4.739399385394688E-5	9.478798770789376E-5	0.0	6.635159139552562E-4	0.0
15	9.478798770789376E-5	9.478798770789376E-5	0.0	9.952738709328845E-4	0.0
16	9.478798770789376E-5	9.478798770789376E-5	0.0	0.001564001797180247	0.0
17	9.478798770789376E-5	9.478798770789376E-5	0.0	0.001990547741865769	0.0
18	9.478798770789376E-5	9.478798770789376E-5	0.0	0.00227491170498945	0.0
19	9.478798770789376E-5	9.478798770789376E-5	0.0	0.002606669661967078	0.0
20	9.478798770789376E-5	9.478798770789376E-5	0.0	0.0032227915820683874	0.0
21	9.478798770789376E-5	9.478798770789376E-5	0.0	0.004265459446855219	0.0
22	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.007725220998193341	0.0
23	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.012938560322127498	0.0
24	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.0212325092465682	0.0
25	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.026635424545918145	0.0
26	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.0348819794765049	0.0
27	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.09748944535756873	0.0
28	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.16422018870392593	0.0
29	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.24715967794833296	0.0
30	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.3886307496023644	0.0
31	9.478798770789376E-5	1.4218198156184064E-4	0.0	0.5942258949407859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5715	0.0	27.321083	1
TAGTGCG	65	2.684612E-6	22.76923	7
GGCGTAA	70	5.1052593E-6	21.142859	19
TATAGCG	45	0.0038268527	20.555555	5
GTATTGG	760	0.0	19.717106	1
CGAACGA	130	6.9667294E-10	18.5	16
ACGATCG	50	0.0070367823	18.5	8
ATTGGAC	730	0.0	18.246574	3
TTGGACC	1185	0.0	16.86076	4
TGGACCC	1190	0.0	16.789917	5
GGACCCT	1230	0.0	16.544716	6
GTATCAA	9900	0.0	15.75303	2
AGTGCGC	95	7.064149E-5	15.578948	8
TATTGGA	970	0.0	15.448455	2
ACGAACG	145	5.3520125E-8	15.310345	15
CGTCGCA	100	1.09405155E-4	14.8	11
AACACCG	75	0.0041059176	14.8	5
GTACCGT	90	8.278057E-4	14.388888	6
GCGCTAG	90	8.278057E-4	14.388888	11
CGTAACG	105	1.6566658E-4	14.095239	21
>>END_MODULE