FastQCFastQC Report
Wed 25 May 2016
SRR2088385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662862
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185061.1129005293283507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179141.0772992587478696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160440.9648425425561472No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83730.5035294570445413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.34879623203849747No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA47150.2835472817347441No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT44780.26929474604627446No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43570.26201813499857474No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT42790.2573274270504708No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA36550.21980176346563934No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35390.2128258388248694No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32230.1938224579069099No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30850.1855235130756491No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28270.17000809447807455No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25390.15268855743892157No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24190.14547208367260783No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA24020.1444497498890467No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.1366319033088735No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20060.12063538646021137No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC19850.11937250355110647No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16830.10121104457255022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.026.9347111
GTATTGG6450.020.077521
ACGTTTA851.2459932E-619.58823626
ATTGGAC6000.019.4253
CTAACGG500.00703617118.527
CGCCGGA1205.178663E-918.514
CGTGGTT1054.8004404E-717.6190536
TATACCG1054.8004404E-717.619055
ACGGACC1655.4569682E-1116.8181828
TACGTTA1107.8127596E-716.81818219
ATCACGT1005.8809655E-616.65000223
TGGACCC9750.016.317955
GCGTCGG1251.6589001E-716.2799999
TTGGACC10050.016.1990054
GGACCCT9400.016.1382986
ACGTTAA1151.2429336E-616.08695620
GACCGTC1151.2429336E-616.08695622
GTATCAA79500.015.56792452
CGGACCA1701.4879333E-915.2352949
GCCGTCA1353.9749466E-715.07407313
Produced by FastQC (version 0.11.5)