##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1662862
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.381500088401804	31.0	31.0	34.0	30.0	34.0
2	31.6200490479667	31.0	31.0	34.0	30.0	34.0
3	31.703595968877753	31.0	31.0	34.0	30.0	34.0
4	35.497852497681706	37.0	35.0	37.0	33.0	37.0
5	35.32263471051717	37.0	35.0	37.0	33.0	37.0
6	35.35983202454563	37.0	35.0	37.0	32.0	37.0
7	35.22737364856494	37.0	35.0	37.0	32.0	37.0
8	35.2532368891706	37.0	35.0	37.0	32.0	37.0
9	36.75934142460409	39.0	37.0	39.0	32.0	39.0
10	36.64542938620282	39.0	35.0	39.0	32.0	39.0
11	36.753833451001945	39.0	35.0	39.0	32.0	39.0
12	36.61629527886259	39.0	35.0	39.0	32.0	39.0
13	36.65265668467979	39.0	35.0	39.0	32.0	39.0
14	37.65603279165679	39.0	36.0	41.0	32.0	41.0
15	37.648508415009786	39.0	36.0	41.0	32.0	41.0
16	37.607704668216606	39.0	36.0	41.0	32.0	41.0
17	37.6723173660833	39.0	36.0	41.0	32.0	41.0
18	37.64285791605076	39.0	36.0	41.0	32.0	41.0
19	37.713708654115614	40.0	37.0	41.0	32.0	41.0
20	37.681115450350056	39.0	37.0	41.0	32.0	41.0
21	37.62230720288274	39.0	37.0	41.0	32.0	41.0
22	37.52151711927989	39.0	36.0	41.0	32.0	41.0
23	37.404492375194096	39.0	36.0	41.0	32.0	41.0
24	37.33889342591267	39.0	36.0	41.0	31.0	41.0
25	37.28432124854618	39.0	36.0	41.0	31.0	41.0
26	37.05867113446576	39.0	36.0	41.0	31.0	41.0
27	36.91399586977151	39.0	35.0	40.0	31.0	41.0
28	36.80948509256932	39.0	35.0	40.0	30.0	41.0
29	36.66436060238312	39.0	35.0	40.0	30.0	41.0
30	36.48777589481268	38.0	35.0	40.0	30.0	41.0
31	36.24085281881479	38.0	35.0	40.0	30.0	41.0
32	36.096814407930424	38.0	35.0	40.0	30.0	41.0
33	36.15600873674424	38.0	35.0	40.0	30.0	41.0
34	36.107763602752364	38.0	35.0	40.0	30.0	41.0
35	36.04189163021345	38.0	35.0	40.0	30.0	41.0
36	35.96151394403144	38.0	35.0	40.0	29.0	41.0
37	35.84619950422825	38.0	35.0	40.0	29.0	41.0
38	35.72681316910243	38.0	35.0	40.0	28.0	41.0
39	35.672127332274115	38.0	35.0	40.0	27.0	41.0
40	35.50996474752565	38.0	34.0	40.0	26.0	41.0
41	35.36390873085079	38.0	34.0	40.0	26.0	41.0
42	35.21505392510022	38.0	34.0	40.0	25.0	41.0
43	34.87917457973061	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	4.0
16	13.0
17	36.0
18	95.0
19	226.0
20	470.0
21	977.0
22	1710.0
23	3068.0
24	5210.0
25	8134.0
26	12460.0
27	18498.0
28	26099.0
29	35480.0
30	45782.0
31	57077.0
32	70297.0
33	87517.0
34	107866.0
35	132308.0
36	168907.0
37	228558.0
38	299526.0
39	352542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31936384378258	22.152048696764975	13.611412131614047	22.91717532783839
2	17.93522252598231	22.870087836513193	36.154894392920156	23.039795244584337
3	19.605174692788697	25.988266013655974	32.11655567329099	22.29000362026434
4	12.984841796853857	15.950872652090192	35.966905251307686	35.09738029974827
5	11.927087154556421	39.82423075396515	34.60040580637479	13.648276285103634
6	31.379994250875896	36.624326011418866	15.852067098773079	16.143612638932154
7	25.078689632693514	34.663489814548655	21.810468938492793	18.447351614265045
8	27.536740872062744	33.466878189531066	20.393454177195704	18.60292676121049
9	25.764254640493316	13.635767730575358	23.136315581208784	37.46366204772254
10	16.500707815801913	27.349292965982748	33.69419711316994	22.455802105045397
11	32.13381507304875	22.141765221647976	24.553330342505873	21.171089362797392
12	20.424304602546695	27.321329130138277	31.3508878066851	20.903478460629927
13	30.65515959833107	20.95447487524521	28.109007241731426	20.281358284692296
14	21.084251128476087	22.5820302586745	28.029144932050887	28.304573680798526
15	23.336392316379833	28.96866967914355	26.09440831530217	21.600529689174447
16	22.220304511138025	28.235295532641913	26.77257643749151	22.771823518728553
17	20.10527632479424	27.16713714066471	27.70422320072261	25.023363333818438
18	21.05712921457102	26.309579508101095	31.35329329794054	21.279997979387346
19	20.5252750979937	25.287005175414436	32.445747151597665	21.741972574994197
20	24.156845246328317	24.284636969273457	31.20812190067486	20.350395883723362
21	23.76980170332836	25.21255522105863	30.124267678255922	20.893375397357087
22	22.483405117201546	24.802418962006467	30.98471190032606	21.72946402046592
23	21.384035476185034	26.223041960186716	31.90270750068256	20.490215062945694
24	20.27504387014677	25.80322359883141	30.700503108496076	23.22122942252574
25	21.410616154557623	26.41073041539226	31.40657492924849	20.77207850080163
26	22.419900148057987	25.912613313672452	30.262703700006377	21.404782838263188
27	21.453494036185806	26.773057535742595	30.03352052064453	21.739927907427077
28	21.084672089445785	26.51825587451033	30.22938764611856	22.16768438992532
29	20.18321424147043	26.43087640465655	31.15814781984314	22.227761534029884
30	19.75148869840071	26.85694904327599	32.41044656742412	20.981115690899184
31	20.959586544163013	26.309038272568618	31.524504138046332	21.206871045222034
32	21.776972472760818	26.295146560568465	30.00230927160522	21.925571695065496
33	22.379127071278315	26.324132730196492	29.685085112294345	21.611655086230847
34	21.269594229707575	28.30144654216646	29.64539450657962	20.783564721546348
35	20.198729660068004	28.985508117931612	30.031956951328493	20.78380527067189
36	20.74772290184032	26.43099667921932	31.413310304763716	21.407970114176642
37	21.006914584613757	26.37302433996327	30.875743146454727	21.74431792896825
38	20.96529958589468	26.025130167145562	31.41084467622689	21.598725570732867
39	21.915769318199587	25.56357653250841	31.289247093264507	21.2314070560275
40	20.42274103323066	26.05513867055715	30.962100282524947	22.560020013687247
41	21.069577631817914	25.76539724883965	30.835330893363373	22.329694225979065
42	19.27556225351232	27.038082534810464	31.26338806226855	22.42296714940867
43	19.603430711628505	26.685798340451583	30.943998960827777	22.76677198709213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	175.5
2	225.0
3	685.0
4	1145.0
5	1145.0
6	1931.0
7	2717.0
8	2947.0
9	3177.0
10	4818.0
11	6459.0
12	6459.0
13	11381.5
14	16304.0
15	26968.5
16	37633.0
17	37044.0
18	36455.0
19	36455.0
20	39330.0
21	42205.0
22	35307.5
23	28410.0
24	31695.5
25	34981.0
26	34981.0
27	37799.5
28	40618.0
29	43245.5
30	45873.0
31	49473.5
32	53074.0
33	53074.0
34	57941.0
35	62808.0
36	68570.5
37	74333.0
38	77028.5
39	79724.0
40	79724.0
41	84667.5
42	89611.0
43	99390.5
44	109170.0
45	130866.0
46	152562.0
47	152562.0
48	156006.5
49	159451.0
50	145414.0
51	131377.0
52	124277.0
53	117177.0
54	117177.0
55	103732.5
56	90288.0
57	77890.0
58	65492.0
59	57949.0
60	50406.0
61	50406.0
62	44907.5
63	39409.0
64	35004.5
65	30600.0
66	26161.0
67	21722.0
68	21722.0
69	18427.5
70	15133.0
71	12733.5
72	10334.0
73	8129.5
74	5925.0
75	5925.0
76	4534.0
77	3143.0
78	2558.0
79	1973.0
80	1635.0
81	1297.0
82	1297.0
83	963.0
84	629.0
85	505.5
86	382.0
87	326.5
88	271.0
89	271.0
90	230.5
91	190.0
92	112.5
93	35.0
94	24.0
95	13.0
96	13.0
97	9.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1662862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.831793670834465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21483903693334	37.41156042812124
2	12.176658257749366	11.648628107698608
3	3.984895368329656	5.718140791734241
4	1.7528805779195435	3.3537368853064278
5	0.8988614238918005	2.149707708313254
6	0.566319637137725	1.6252850421188108
7	0.36602989244840856	1.2255506405065006
8	0.2802785095946554	1.0724979073040444
9	0.2147371623915851	0.9244137280477297
>10	1.3134051402539413	11.979225517467734
>50	0.13101725629395392	4.305771145269133
>100	0.08835592660737376	7.841015700243878
>500	0.006554130573476082	2.2785117441423206
>1k	0.0045374750124065185	4.448565991652583
>5k	2.5208194513369546E-4	0.8544569759678551
>10k+	3.7812291770054316E-4	3.1629316861058028
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18506	1.1129005293283507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17914	1.0772992587478696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16044	0.9648425425561472	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8373	0.5035294570445413	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5800	0.34879623203849747	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4715	0.2835472817347441	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4478	0.26929474604627446	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4357	0.26201813499857474	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4279	0.2573274270504708	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3655	0.21980176346563934	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3539	0.2128258388248694	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3223	0.1938224579069099	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3085	0.1855235130756491	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2827	0.17000809447807455	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2539	0.15268855743892157	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2419	0.14547208367260783	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2402	0.1444497498890467	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2272	0.1366319033088735	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2006	0.12063538646021137	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1985	0.11937250355110647	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1683	0.10121104457255022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2027456277189569E-4	0.0
11	0.0	0.0	0.0	4.8109825108758275E-4	0.0
12	0.0	0.0	0.0	4.8109825108758275E-4	0.0
13	0.0	0.0	0.0	5.412355324735306E-4	0.0
14	0.0	0.0	0.0	6.615100952454263E-4	0.0
15	0.0	0.0	0.0	7.817846580173219E-4	0.0
16	0.0	0.0	0.0	0.0013831574718768003	0.0
17	0.0	0.0	0.0	0.0028264522251395486	0.0
18	0.0	0.0	0.0	0.0033676877576130793	0.0
19	0.0	0.0	0.0	0.0036683741645428185	0.0
20	0.0	0.0	0.0	0.004510296103946088	0.0
21	0.0	0.0	0.0	0.006915787359384002	0.0
22	0.0	0.0	0.0	0.010704436086698715	0.0
23	0.0	0.0	0.0	0.016537752381135657	0.0
24	0.0	0.0	0.0	0.02621985468427326	0.0
25	0.0	0.0	0.0	0.03241399466702589	0.0
26	0.0	0.0	0.0	0.04317856803511055	0.0
27	0.0	0.0	0.0	0.11468179560300254	0.0
28	0.0	0.0	0.0	0.19021422102375302	0.0
29	0.0	0.0	0.0	0.2784356128169385	0.0
30	0.0	0.0	0.0	0.4367770747061392	0.0
31	6.0137281385947844E-5	0.0	0.0	0.6683056080420383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4595	0.0	26.934711	1
GTATTGG	645	0.0	20.07752	1
ACGTTTA	85	1.2459932E-6	19.588236	26
ATTGGAC	600	0.0	19.425	3
CTAACGG	50	0.007036171	18.5	27
CGCCGGA	120	5.178663E-9	18.5	14
CGTGGTT	105	4.8004404E-7	17.61905	36
TATACCG	105	4.8004404E-7	17.61905	5
ACGGACC	165	5.4569682E-11	16.818182	8
TACGTTA	110	7.8127596E-7	16.818182	19
ATCACGT	100	5.8809655E-6	16.650002	23
TGGACCC	975	0.0	16.31795	5
GCGTCGG	125	1.6589001E-7	16.279999	9
TTGGACC	1005	0.0	16.199005	4
GGACCCT	940	0.0	16.138298	6
ACGTTAA	115	1.2429336E-6	16.086956	20
GACCGTC	115	1.2429336E-6	16.086956	22
GTATCAA	7950	0.0	15.5679245	2
CGGACCA	170	1.4879333E-9	15.235294	9
GCCGTCA	135	3.9749466E-7	15.074073	13
>>END_MODULE