FastQCFastQC Report
Wed 25 May 2016
SRR2088384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876576
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161880.8626349265897038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155650.829436164589124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144700.7710852105110584No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG80280.4278004194874068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.28472068277543783No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46430.24741870299950547No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44280.23596166635404056No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT40630.2165113483280187No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT39720.2116620909571475No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34400.1833125863274389No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT33470.17835675187149364No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30320.16157086097232407No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT28400.15133946080521118No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26940.14355933359480247No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25880.13791074808587556No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22310.11888673840015007No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.1077494330099074No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT20090.10705668195692579No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18990.10119494227785071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.028.2211171
CGAACGA1001.2911187E-820.3516
ATTGGAC4750.019.4736843
GTATTGG5450.016.9724771
GTATCAA68550.016.6783372
ACGAACG1251.6592458E-716.2815
ACGTTTA803.3839917E-416.187526
TTGGACC8950.015.5027934
TGGACCC8300.015.37951855
TCCGATA1455.350921E-815.3103458
AACGAAC1455.350921E-815.31034514
GGACCCT8600.015.2732576
CGATAAC1353.975747E-715.07407310
TAACGAA1508.109055E-814.813
CCGCCTA750.004105692714.89
CTAGATC800.00630057913.8750013
TATTGGA8850.013.796612
CCGATAA1501.3066419E-613.5666679
ATTGACG2051.4242687E-913.53658532
CTCGTTA1102.4576587E-413.45454511
Produced by FastQC (version 0.11.5)