##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876576
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420316576573505	31.0	31.0	34.0	30.0	34.0
2	31.662462911174394	31.0	31.0	34.0	30.0	34.0
3	31.758630079463874	31.0	31.0	34.0	30.0	34.0
4	35.54028933547056	37.0	35.0	37.0	33.0	37.0
5	35.35132283477994	37.0	35.0	37.0	33.0	37.0
6	35.38945611581945	37.0	35.0	37.0	33.0	37.0
7	35.269897408897904	37.0	35.0	37.0	32.0	37.0
8	35.28743466824685	37.0	35.0	37.0	32.0	37.0
9	36.824046561396926	39.0	37.0	39.0	32.0	39.0
10	36.702177796156406	39.0	35.0	39.0	32.0	39.0
11	36.80040350084409	39.0	37.0	39.0	32.0	39.0
12	36.67119423886909	39.0	35.0	39.0	32.0	39.0
13	36.715395486247296	39.0	35.0	39.0	32.0	39.0
14	37.76227821308596	40.0	37.0	41.0	32.0	41.0
15	37.749809759903144	40.0	37.0	41.0	32.0	41.0
16	37.70950070767184	39.0	37.0	41.0	32.0	41.0
17	37.73352371553297	39.0	37.0	41.0	32.0	41.0
18	37.69839590829255	39.0	37.0	41.0	32.0	41.0
19	37.75668451477585	40.0	37.0	41.0	32.0	41.0
20	37.72425150913153	40.0	37.0	41.0	32.0	41.0
21	37.67252805108879	39.0	37.0	41.0	32.0	41.0
22	37.578190811350034	39.0	36.0	41.0	32.0	41.0
23	37.479417833330494	39.0	36.0	41.0	32.0	41.0
24	37.40347153539212	39.0	36.0	41.0	32.0	41.0
25	37.34658921354637	39.0	36.0	41.0	31.0	41.0
26	37.138998367239054	39.0	36.0	41.0	31.0	41.0
27	36.993106061251986	39.0	36.0	40.0	31.0	41.0
28	36.89255697610968	39.0	35.0	40.0	31.0	41.0
29	36.75872066998619	39.0	35.0	40.0	30.0	41.0
30	36.60050432276657	38.0	35.0	40.0	30.0	41.0
31	36.39968804887199	38.0	35.0	40.0	30.0	41.0
32	36.28172320225773	38.0	35.0	40.0	30.0	41.0
33	36.36071014443326	38.0	35.0	40.0	30.0	41.0
34	36.332977721126134	38.0	35.0	40.0	30.0	41.0
35	36.2706093438262	38.0	35.0	40.0	30.0	41.0
36	36.19747081919411	38.0	35.0	40.0	30.0	41.0
37	36.100542690517194	38.0	35.0	40.0	29.0	41.0
38	35.99166247463465	38.0	35.0	40.0	29.0	41.0
39	35.97433623791416	38.0	35.0	40.0	29.0	41.0
40	35.83290578159371	38.0	35.0	40.0	28.0	41.0
41	35.714046220350255	38.0	35.0	40.0	27.0	41.0
42	35.59753774960353	38.0	35.0	40.0	27.0	41.0
43	35.285128873011274	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	8.0
16	20.0
17	38.0
18	101.0
19	186.0
20	469.0
21	931.0
22	1764.0
23	3224.0
24	5228.0
25	8317.0
26	12702.0
27	19449.0
28	27422.0
29	37539.0
30	48950.0
31	61955.0
32	76536.0
33	95078.0
34	118791.0
35	147143.0
36	187325.0
37	253163.0
38	346276.0
39	423957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4703694388077	21.642342223283258	13.187741929983119	23.699546407925926
2	18.08410637245707	22.603933973364253	35.94567979127944	23.36627986289924
3	19.50595126443054	25.82922301041898	31.521878144024008	23.142947581126478
4	13.164827856692188	15.941587231212592	35.88210656003274	35.01147835206248
5	12.503730197977594	39.041264515798986	34.68913595825589	13.765869327967534
6	31.86729447674914	36.43241733881282	16.03217775352557	15.66811043091247
7	25.73623450369183	33.79633971659022	22.106218986068242	18.36120679364971
8	27.241955561618607	33.378504254557235	20.654319356103883	18.725220827720275
9	25.813023293487714	13.834131950957488	22.476041471275344	37.876803284279454
10	16.481187012942723	26.888226216257692	33.945227904438724	22.68535886636086
11	32.8985876404686	22.122631857169655	24.29227486656549	20.68650563579626
12	20.498450369183022	26.826518083999794	31.162553501696706	21.512478045120474
13	30.217161468546973	20.774698173695068	27.72661485599304	21.281525501764918
14	21.253389151305356	21.9601550909742	27.612843817676445	29.173611940043997
15	23.496090752519482	28.869121207987313	25.37904140306601	22.255746636427194
16	22.84772905547124	27.88157793769077	26.1936100642873	23.07708294255069
17	20.784343399894276	27.080544566273897	27.144863837116105	24.99024819671572
18	21.850540558975496	25.991220179731595	30.280628122708592	21.877611138584317
19	21.278701209010453	25.159492607813377	30.97337917569019	22.588427007485976
20	24.29222157802295	24.50660138464949	29.91480227819177	21.286374759135786
21	24.141681445355797	25.241663540405508	29.06921968521392	21.54743532902478
22	22.923718517129068	24.955930375321863	29.862739372133078	22.257611735415992
23	22.0333735484201	26.049411268182048	30.61330849376737	21.303906689630477
24	21.083185546442028	25.694829306140544	29.486309107651383	23.735676039766044
25	22.12486997595621	26.15710741264942	30.088149907064782	21.629872704329586
26	22.87389372985693	25.75520522483502	29.319409392425356	22.051491652882696
27	22.090605443104888	26.658392732295415	28.95955186467268	22.291449959927014
28	21.72573879235373	26.295231314905443	29.25706179765701	22.72196809508381
29	20.970160547720955	26.356193407567822	29.87728714424569	22.79635890046553
30	20.672330883481404	26.529114728100538	30.9548347628873	21.843719625530753
31	21.69312620432106	26.030227392868717	30.347505243592586	21.92914115921764
32	22.382200347867606	25.970384359599613	28.885107770748427	22.762307521784354
33	22.728256143103184	26.004169295568097	28.834643520965848	22.432931040362874
34	21.95344073461453	27.83761489009771	28.71650282216121	21.492441553126547
35	21.203830806745902	28.185002898896716	29.11094461402043	21.500221680336953
36	21.483915386320618	26.21178145729243	30.341057329945603	21.963245826441348
37	21.824056153334585	25.952692563477314	29.8525612605085	22.370690022679604
38	21.4755490851423	25.76906024589465	30.429569599099636	22.325821069863412
39	22.363975666319934	25.388899783435363	30.359655031291034	21.88746951895367
40	21.202392016097402	25.736714100574666	30.122734171171324	22.938159712156608
41	21.524734409903996	25.47677259007895	30.266400081851202	22.73209291816585
42	20.192307692307693	26.66334856675136	30.462395341302457	22.68194839963849
43	20.35755546271507	26.181193833876165	30.126677523319067	23.334573180089695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	194.5
2	274.0
3	789.5
4	1305.0
5	1305.0
6	2108.5
7	2912.0
8	3006.0
9	3100.0
10	4533.0
11	5966.0
12	5966.0
13	10508.0
14	15050.0
15	24358.5
16	33667.0
17	33522.0
18	33377.0
19	33377.0
20	36988.5
21	40600.0
22	35079.5
23	29559.0
24	32929.5
25	36300.0
26	36300.0
27	39467.0
28	42634.0
29	48186.0
30	53738.0
31	56267.5
32	58797.0
33	58797.0
34	63512.0
35	68227.0
36	75948.5
37	83670.0
38	88309.5
39	92949.0
40	92949.0
41	99714.0
42	106479.0
43	114841.5
44	123204.0
45	142446.0
46	161688.0
47	161688.0
48	165534.0
49	169380.0
50	158495.5
51	147611.0
52	140660.5
53	133710.0
54	133710.0
55	119832.0
56	105954.0
57	93721.5
58	81489.0
59	74341.0
60	67193.0
61	67193.0
62	60255.0
63	53317.0
64	47242.5
65	41168.0
66	35222.5
67	29277.0
68	29277.0
69	24910.5
70	20544.0
71	17510.5
72	14477.0
73	11322.0
74	8167.0
75	8167.0
76	6245.0
77	4323.0
78	3466.5
79	2610.0
80	2114.5
81	1619.0
82	1619.0
83	1213.0
84	807.0
85	683.0
86	559.0
87	477.5
88	396.0
89	396.0
90	340.0
91	284.0
92	171.0
93	58.0
94	35.0
95	12.0
96	12.0
97	8.0
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1876576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.70360763545883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0209071691097	36.438578357886556
2	12.112424038641588	11.313877996300327
3	3.90336388771599	5.469035264109203
4	1.7366848002313597	3.244377819858825
5	0.9771877590012549	2.2819096841283955
6	0.5814169091170097	1.629256031761323
7	0.4131477484076762	1.3506843235973758
8	0.29511100826368497	1.102619899108143
9	0.2393525022755039	1.006076281755636
>10	1.4581148961630293	13.046059358102044
>50	0.14922232534690827	4.8238830395055246
>100	0.10105115993477179	9.015235427688086
>500	0.008010530527665817	2.657058477643487
>1k	0.003433084511856778	3.4362983972661776
>5k	2.288723007904519E-4	0.714623934518175
>10k+	3.4330845118567786E-4	2.470425706770892
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16188	0.8626349265897038	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15565	0.829436164589124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14470	0.7710852105110584	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8028	0.4278004194874068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5343	0.28472068277543783	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4643	0.24741870299950547	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4428	0.23596166635404056	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4063	0.2165113483280187	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3972	0.2116620909571475	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3440	0.1833125863274389	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3347	0.17835675187149364	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3032	0.16157086097232407	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2840	0.15133946080521118	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2694	0.14355933359480247	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2588	0.13791074808587556	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2231	0.11888673840015007	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2022	0.1077494330099074	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2009	0.10705668195692579	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1899	0.10119494227785071	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0657708507409239E-4	0.0	0.0	0.0	0.0
9	1.0657708507409239E-4	0.0	0.0	5.3288542537046195E-5	0.0
10	1.0657708507409239E-4	0.0	0.0	1.5986562761113857E-4	0.0
11	1.0657708507409239E-4	0.0	0.0	4.7959688283341574E-4	0.0
12	1.0657708507409239E-4	0.0	0.0	6.927510529816006E-4	0.0
13	1.0657708507409239E-4	5.3288542537046195E-5	0.0	7.993281380556929E-4	0.0
14	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.0010657708507409238	0.0
15	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.001332213563426155	0.0
16	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.00223811878655594	0.0
17	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.0028242927544634483	0.0
18	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.003303889637296864	0.0
19	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.003836775062667326	0.0
20	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.004689391743260065	0.0
21	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.0064479136469825895	0.0
22	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.010497842879798101	0.0
23	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.016999045069317736	0.0
24	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.027230445236430607	0.0
25	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.032559299490135224	0.0
26	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.04135190900874785	0.0
27	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.10860204969050015	0.0
28	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.18176721859386458	0.0
29	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.27001304503521306	0.0
30	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.40946916085466295	0.0
31	1.0657708507409239E-4	5.3288542537046195E-5	0.0	0.6313626519789233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4025	0.0	28.221117	1
CGAACGA	100	1.2911187E-8	20.35	16
ATTGGAC	475	0.0	19.473684	3
GTATTGG	545	0.0	16.972477	1
GTATCAA	6855	0.0	16.678337	2
ACGAACG	125	1.6592458E-7	16.28	15
ACGTTTA	80	3.3839917E-4	16.1875	26
TTGGACC	895	0.0	15.502793	4
TGGACCC	830	0.0	15.3795185	5
TCCGATA	145	5.350921E-8	15.310345	8
AACGAAC	145	5.350921E-8	15.310345	14
GGACCCT	860	0.0	15.273257	6
CGATAAC	135	3.975747E-7	15.074073	10
TAACGAA	150	8.109055E-8	14.8	13
CCGCCTA	75	0.0041056927	14.8	9
CTAGATC	80	0.006300579	13.875001	3
TATTGGA	885	0.0	13.79661	2
CCGATAA	150	1.3066419E-6	13.566667	9
ATTGACG	205	1.4242687E-9	13.536585	32
CTCGTTA	110	2.4576587E-4	13.454545	11
>>END_MODULE