FastQCFastQC Report
Wed 25 May 2016
SRR2088383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050974
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149071.4183985522001497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140891.340565989263293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134111.2760544028681966No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76090.7239950750446729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.45662404588505523No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42740.406670383853454No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41090.3909706615006651No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT40580.3861180200461667No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT39410.37498548965055273No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT33250.3163731928668074No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA32850.31256719956916157No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27280.25956874289944376No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT27230.25909299373723804No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25150.23930182858947985No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25130.23911152892459758No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA20590.1959135049963177No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT19900.1893481665578787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18210.17326784487532518No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17610.16755885492885647No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC16180.1539524288897727No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16130.153476679727567No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15490.14738709045133372No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14470.13768180754233691No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14290.1359691105583963No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13160.1252171794925469No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC11940.11360889993472723No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC11390.10837565915046424No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.10694841166384707No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG11130.10590176350699446No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.10504541501502415No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA10750.10228606987423094No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA10590.10076367255517263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36050.028.0707361
ATTGGAC3750.025.6533363
ACGTTTA451.3231071E-424.66666626
CCGTATA400.001931077823.1252
GTATTGG4950.022.424241
ATCACGT502.7018884E-422.223
TAGACTG909.476389E-820.5555555
TCGCTAC450.00382559420.55555527
TATTGGA5500.019.509092
TTGGACC7400.018.754
CGGAAAT609.2360954E-418.525
TTAGATC500.007034490418.53
CGTCTGT3100.018.534
TCTATAC500.007034490418.53
GGACTGA1156.4033884E-817.6956526
TGGACCC7550.017.6423855
GGACCCT7550.017.6423856
GTTCTAC650.001579811917.0769231
TGCGTCT3400.016.86764532
GTATCAA62400.016.2764432
Produced by FastQC (version 0.11.5)