##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050974
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37598170839621	31.0	31.0	34.0	30.0	34.0
2	31.60814825105093	31.0	31.0	34.0	30.0	34.0
3	31.68940430495902	31.0	31.0	34.0	30.0	34.0
4	35.48159802240588	37.0	35.0	37.0	33.0	37.0
5	35.32108406107097	37.0	35.0	37.0	33.0	37.0
6	35.37426044792735	37.0	35.0	37.0	33.0	37.0
7	35.23362994707767	37.0	35.0	37.0	32.0	37.0
8	35.274747044170454	37.0	35.0	37.0	32.0	37.0
9	36.75130878594523	39.0	37.0	39.0	32.0	39.0
10	36.66253970126758	39.0	35.0	39.0	32.0	39.0
11	36.77511432252368	39.0	37.0	39.0	32.0	39.0
12	36.63108031216757	39.0	35.0	39.0	32.0	39.0
13	36.659747053685436	39.0	35.0	39.0	32.0	39.0
14	37.64136696055278	39.0	36.0	41.0	32.0	41.0
15	37.62597457215878	39.0	36.0	41.0	32.0	41.0
16	37.57977171652201	39.0	36.0	41.0	32.0	41.0
17	37.6775581508201	39.0	37.0	41.0	32.0	41.0
18	37.667985126178195	39.0	37.0	41.0	32.0	41.0
19	37.751842576505226	40.0	37.0	41.0	32.0	41.0
20	37.72227666907079	39.0	37.0	41.0	32.0	41.0
21	37.66521721755248	39.0	37.0	41.0	32.0	41.0
22	37.556386742202946	39.0	36.0	41.0	32.0	41.0
23	37.427022933012616	39.0	36.0	41.0	32.0	41.0
24	37.36432870841715	39.0	36.0	41.0	32.0	41.0
25	37.30916844755436	39.0	36.0	41.0	31.0	41.0
26	37.073927613813474	39.0	36.0	41.0	31.0	41.0
27	36.93627815721417	39.0	35.0	40.0	31.0	41.0
28	36.81575757345092	39.0	35.0	40.0	31.0	41.0
29	36.66114290172735	39.0	35.0	40.0	30.0	41.0
30	36.47452172936723	38.0	35.0	40.0	30.0	41.0
31	36.184117780268586	38.0	35.0	40.0	30.0	41.0
32	36.033529849453934	38.0	35.0	40.0	30.0	41.0
33	36.04692694586165	38.0	35.0	40.0	30.0	41.0
34	35.97343987577238	38.0	35.0	40.0	30.0	41.0
35	35.87431563483017	38.0	35.0	40.0	29.0	41.0
36	35.78532009355131	38.0	35.0	40.0	29.0	41.0
37	35.6481302106427	38.0	35.0	40.0	27.0	41.0
38	35.51057019488589	38.0	34.0	40.0	27.0	41.0
39	35.42192670798707	38.0	34.0	40.0	26.0	41.0
40	35.226095983344976	38.0	34.0	40.0	25.0	41.0
41	35.04178219442156	38.0	34.0	40.0	24.0	41.0
42	34.85191070378525	38.0	34.0	40.0	24.0	41.0
43	34.51553606464099	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	3.0
16	9.0
17	29.0
18	66.0
19	121.0
20	285.0
21	579.0
22	1121.0
23	1932.0
24	3300.0
25	5234.0
26	7938.0
27	11873.0
28	17013.0
29	23391.0
30	30493.0
31	37137.0
32	45059.0
33	55932.0
34	68941.0
35	83533.0
36	106907.0
37	148115.0
38	187243.0
39	214718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89742086864185	22.2064484944442	13.760759067303283	22.135371569610665
2	17.904819719612476	22.722921784934734	36.64771916336655	22.724539332086234
3	19.855867033818154	26.175243155396803	32.32715557187904	21.641734238906004
4	12.698125738600574	15.538538536633636	35.22361162121994	36.53972410354586
5	11.359272446321222	40.34400470420771	34.4626984111881	13.834024438282963
6	31.332744673036633	35.87386557612272	15.612565106272847	17.180824644567803
7	24.80289712209817	35.14330516263961	21.320508404584697	18.733289310677524
8	28.054071746779652	33.03012253395422	20.221432690057036	18.694373029209096
9	25.546493062625718	13.487869347862077	23.658434937496075	37.30720265201613
10	16.531427038156984	27.264518437183032	33.406535271091386	22.797519253568595
11	31.293067192908673	21.68873825613193	25.32403275437832	21.694161796581078
12	20.646371841739185	27.31599449653369	31.549686291002445	20.48794737072468
13	31.24606317568275	20.821257233766012	28.81669765379543	19.11598193675581
14	21.18996283447545	22.43671108895177	28.262735329323085	28.11059074724969
15	23.065175732225534	28.522684671552295	27.242063076726925	21.170076519495247
16	21.86733449162396	28.284334341287227	27.1321650202574	22.716166146831416
17	19.374408881665957	26.809797387946798	27.8418876204359	25.97390610995134
18	20.74808701261877	25.966008673858727	32.55066252828328	20.735241785239218
19	19.874992150138823	24.359689202587315	33.869914955079764	21.895403692194098
20	24.645424149408075	23.21684456513672	32.54171844403382	19.596012841421388
21	24.10858879477513	24.25968672869167	31.16718396458904	20.46454051194416
22	22.20730484293617	23.860533181601067	32.23695353072483	21.69520844473793
23	20.932867987219474	25.895597797852275	33.51862177370706	19.65291244122119
24	19.692494771516706	24.982920605076814	31.801643047306595	23.522941576099885
25	20.91022232709848	25.98151809654663	32.8557128910896	20.252546685265287
26	22.578769788786403	24.894336111074107	31.395448412615345	21.131445687524145
27	20.991004534841014	26.492662996420464	30.96460997132184	21.55172249741668
28	20.67824703560697	25.75715479165041	31.18088554046056	22.383712632282055
29	19.56299584956431	25.859155412027317	32.40023064319384	22.177618095214534
30	19.16888524359309	26.16220762835237	34.10179509673883	20.567112031315713
31	20.43885005718505	25.565713328778827	33.01965605238569	20.97578056165043
32	21.835078698426415	25.657342617419648	30.685154913442197	21.82242377071174
33	22.669637878767695	25.425652775425462	30.48543541514823	21.41927393065861
34	20.788430541573817	28.569878988443104	30.39551882349135	20.24617164649173
35	19.52160567245241	29.617098044290348	30.558795935960358	20.30250034729689
36	20.305259692437684	25.803873359379015	32.85714013857622	21.03372680960709
37	20.69137771248385	26.121578649899995	31.631895746231592	21.555147891384564
38	20.85103913132009	25.510811875460288	32.449898855728115	21.188250137491508
39	21.837076844907678	25.042579550017415	32.357698668092645	20.762644936982266
40	20.05168538898203	25.85335127224841	31.389929722333758	22.705033616435802
41	21.012508396972713	25.433169612188312	31.287263053129767	22.267058937709212
42	18.59827169844354	27.129215375451725	31.85016946185158	22.42234346425316
43	19.150521325931948	26.93177947313635	31.40648579317852	22.51121340775319
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	115.0
2	155.0
3	464.5
4	774.0
5	774.0
6	1382.0
7	1990.0
8	2217.0
9	2444.0
10	3632.0
11	4820.0
12	4820.0
13	8781.5
14	12743.0
15	20929.0
16	29115.0
17	28562.0
18	28009.0
19	28009.0
20	29300.5
21	30592.0
22	24041.5
23	17491.0
24	19321.0
25	21151.0
26	21151.0
27	22734.5
28	24318.0
29	25160.0
30	26002.0
31	27808.0
32	29614.0
33	29614.0
34	31905.0
35	34196.0
36	37769.5
37	41343.0
38	43475.0
39	45607.0
40	45607.0
41	48008.0
42	50409.0
43	58540.5
44	66672.0
45	86042.5
46	105413.0
47	105413.0
48	107141.5
49	108870.0
50	99234.0
51	89598.0
52	84993.0
53	80388.0
54	80388.0
55	69747.0
56	59106.0
57	48526.5
58	37947.0
59	33668.0
60	29389.0
61	29389.0
62	25773.0
63	22157.0
64	19452.5
65	16748.0
66	14437.5
67	12127.0
68	12127.0
69	10171.5
70	8216.0
71	7026.0
72	5836.0
73	4559.5
74	3283.0
75	3283.0
76	2569.0
77	1855.0
78	1462.5
79	1070.0
80	839.5
81	609.0
82	609.0
83	458.5
84	308.0
85	268.5
86	229.0
87	194.0
88	159.0
89	159.0
90	134.5
91	110.0
92	66.5
93	23.0
94	16.0
95	9.0
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1050974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.4377158501068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19291360272743	40.139921917339095
2	10.807648472571556	10.686109083896666
3	3.1703456050488423	4.702039355071188
4	1.3473118242693352	2.6643207651886565
5	0.7550474586823258	1.8663910857841037
6	0.4949574762885918	1.4681740242384822
7	0.33635642602483995	1.1640085389921455
8	0.23377496621752447	0.9245840282184232
9	0.2033310606105447	0.9047000878168456
>10	1.264710188751612	12.05218178946491
>50	0.11601076451633069	3.949289331225556
>100	0.06272997558794272	5.655969539307557
>500	0.008685688852515786	3.0684968123199643
>1k	0.005404428619343156	5.981168911684164
>5k	1.9301530783368418E-4	0.7260687329335015
>10k+	5.790459235010525E-4	4.046575996518728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14907	1.4183985522001497	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14089	1.340565989263293	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13411	1.2760544028681966	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7609	0.7239950750446729	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4799	0.45662404588505523	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4274	0.406670383853454	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4109	0.3909706615006651	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4058	0.3861180200461667	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3941	0.37498548965055273	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3325	0.3163731928668074	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3285	0.31256719956916157	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2728	0.25956874289944376	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2723	0.25909299373723804	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2515	0.23930182858947985	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2513	0.23911152892459758	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2059	0.1959135049963177	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1990	0.1893481665578787	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.17326784487532518	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1761	0.16755885492885647	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1618	0.1539524288897727	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1613	0.153476679727567	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1549	0.14738709045133372	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1447	0.13768180754233691	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1429	0.1359691105583963	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1316	0.1252171794925469	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1194	0.11360889993472723	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1139	0.10837565915046424	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.10694841166384707	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1113	0.10590176350699446	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.10504541501502415	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1075	0.10228606987423094	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1059	0.10076367255517263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.708989946468704E-4	0.0
12	0.0	0.0	0.0	0.0011417979892937408	0.0
13	9.514983244114507E-5	0.0	9.514983244114507E-5	0.0012369478217348858	0.0
14	9.514983244114507E-5	0.0	9.514983244114507E-5	0.0015223973190583212	0.0
15	9.514983244114507E-5	0.0	9.514983244114507E-5	0.0024738956434697717	0.0
16	9.514983244114507E-5	0.0	9.514983244114507E-5	0.004091442794969238	0.0
17	9.514983244114507E-5	0.0	9.514983244114507E-5	0.005994439443792139	0.0
18	9.514983244114507E-5	0.0	9.514983244114507E-5	0.007136237433085881	0.0
19	9.514983244114507E-5	0.0	9.514983244114507E-5	0.008468335087261912	0.0
20	9.514983244114507E-5	0.0	9.514983244114507E-5	0.010561631400967103	0.0
21	9.514983244114507E-5	0.0	9.514983244114507E-5	0.015128823358142066	0.0
22	9.514983244114507E-5	0.0	9.514983244114507E-5	0.025405005261785733	0.0
23	9.514983244114507E-5	0.0	9.514983244114507E-5	0.044910720912220474	0.0
24	9.514983244114507E-5	0.0	9.514983244114507E-5	0.06955452751447705	0.0
25	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.07821316226662124	0.0
26	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.09124868931105812	0.0
27	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.1657510081124747	0.0
28	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.24757986401185947	0.0
29	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.34482299276670975	0.0
30	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.5255125245724442	0.0
31	9.514983244114507E-5	0.0	1.9029966488229015E-4	0.769952444113746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3605	0.0	28.070736	1
ATTGGAC	375	0.0	25.653336	3
ACGTTTA	45	1.3231071E-4	24.666666	26
CCGTATA	40	0.0019310778	23.125	2
GTATTGG	495	0.0	22.42424	1
ATCACGT	50	2.7018884E-4	22.2	23
TAGACTG	90	9.476389E-8	20.555555	5
TCGCTAC	45	0.003825594	20.555555	27
TATTGGA	550	0.0	19.50909	2
TTGGACC	740	0.0	18.75	4
CGGAAAT	60	9.2360954E-4	18.5	25
TTAGATC	50	0.0070344904	18.5	3
CGTCTGT	310	0.0	18.5	34
TCTATAC	50	0.0070344904	18.5	3
GGACTGA	115	6.4033884E-8	17.695652	6
TGGACCC	755	0.0	17.642385	5
GGACCCT	755	0.0	17.642385	6
GTTCTAC	65	0.0015798119	17.076923	1
TGCGTCT	340	0.0	16.867645	32
GTATCAA	6240	0.0	16.276443	2
>>END_MODULE