FastQCFastQC Report
Wed 25 May 2016
SRR2088382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261809
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207120.9157271900500882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT198900.8793845987879613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182840.8083794873926137No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG92980.4110868778044477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69910.30908887532059515No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51180.22627905362477557No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT47910.21182159943655718No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT47410.20961097953010177No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46250.20448234134712523No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA40200.1777338404790148No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT37770.16699022773364153No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT35480.15686558856207575No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG33980.15023372884270952No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31850.1408164880412095No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29400.129984450499578No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26560.11742812943091127No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT26260.11610175748703803No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.11499644753381033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.028.0856861
TCTAACG852.725004E-517.4117642
GTATTGG7250.016.3310361
GTATCAA86000.016.1767442
ATTGGAC7050.015.744683
CGTTTAG1557.219569E-915.516129526
TTGGACC10450.015.4019144
GTCTATA1455.3525582E-815.3103451
TTACGCT750.004106038714.84
CGCCCAA1001.0941071E-414.821
ACGCCTA1152.2116046E-514.478266
GTATACG1551.2116652E-714.3225811
TAATACG800.006301105513.8750014
AATACGC800.006301105513.8750015
GGACCCT10950.013.8538816
TATACGG1753.5704943E-813.7428572
GCGTGCG2457.2759576E-1213.5918369
CCCGTAT1102.458008E-413.4545451
AATGCGT1652.5981353E-713.45454435
TAACACT2355.638867E-1113.38297754
Produced by FastQC (version 0.11.5)