##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2261809
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386145337647875	31.0	31.0	34.0	30.0	34.0
2	31.632934964888722	31.0	31.0	34.0	30.0	34.0
3	31.722179901132236	31.0	31.0	34.0	30.0	34.0
4	35.50885552228327	37.0	35.0	37.0	33.0	37.0
5	35.315803854348445	37.0	35.0	37.0	33.0	37.0
6	35.35721318643617	37.0	35.0	37.0	32.0	37.0
7	35.232969715833654	37.0	35.0	37.0	32.0	37.0
8	35.24554593248148	37.0	35.0	37.0	32.0	37.0
9	36.778773097109436	39.0	37.0	39.0	32.0	39.0
10	36.65305514302932	39.0	35.0	39.0	32.0	39.0
11	36.76776023085946	39.0	35.0	39.0	32.0	39.0
12	36.62302652434401	39.0	35.0	39.0	32.0	39.0
13	36.66570033101822	39.0	35.0	39.0	32.0	39.0
14	37.693712864348846	39.0	37.0	41.0	32.0	41.0
15	37.68475189549604	39.0	36.0	41.0	32.0	41.0
16	37.65092852667931	39.0	36.0	41.0	32.0	41.0
17	37.68460555245823	39.0	37.0	41.0	32.0	41.0
18	37.65212137718083	39.0	36.0	41.0	32.0	41.0
19	37.71690005654766	40.0	37.0	41.0	32.0	41.0
20	37.679724503704776	39.0	37.0	41.0	32.0	41.0
21	37.62617179434692	39.0	37.0	41.0	32.0	41.0
22	37.528759943920996	39.0	36.0	41.0	32.0	41.0
23	37.423733392165296	39.0	36.0	41.0	32.0	41.0
24	37.35332337964877	39.0	36.0	41.0	31.0	41.0
25	37.30306405182754	39.0	36.0	41.0	31.0	41.0
26	37.08098782876892	39.0	36.0	41.0	31.0	41.0
27	36.94234305372381	39.0	35.0	40.0	31.0	41.0
28	36.848507102058576	39.0	35.0	40.0	30.0	41.0
29	36.70564623272787	39.0	35.0	40.0	30.0	41.0
30	36.54012783572795	38.0	35.0	40.0	30.0	41.0
31	36.31380235908514	38.0	35.0	40.0	30.0	41.0
32	36.1789819564782	38.0	35.0	40.0	30.0	41.0
33	36.254258869780784	38.0	35.0	40.0	30.0	41.0
34	36.22375010445179	38.0	35.0	40.0	30.0	41.0
35	36.175799548060866	38.0	35.0	40.0	30.0	41.0
36	36.106063774615805	38.0	35.0	40.0	30.0	41.0
37	36.002264116908194	38.0	35.0	40.0	29.0	41.0
38	35.88845963562794	38.0	35.0	40.0	29.0	41.0
39	35.85413976158022	38.0	35.0	40.0	28.0	41.0
40	35.71446660615463	38.0	35.0	40.0	27.0	41.0
41	35.588412637848734	38.0	35.0	40.0	27.0	41.0
42	35.475632999957114	38.0	34.0	40.0	26.0	41.0
43	35.151209938593404	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	3.0
16	19.0
17	43.0
18	82.0
19	234.0
20	609.0
21	1190.0
22	2287.0
23	3908.0
24	6457.0
25	10491.0
26	16316.0
27	23891.0
28	34058.0
29	46536.0
30	60912.0
31	76777.0
32	94877.0
33	117754.0
34	145391.0
35	178145.0
36	227809.0
37	306471.0
38	414835.0
39	492707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.317547149206675	21.793705834577544	13.295773427376052	23.59297358883973
2	18.08667310104434	22.73728683544897	35.707435950604136	23.46860411290255
3	19.5813616445951	25.686740127039904	31.57998752326125	23.151910705103745
4	13.334061364155858	16.11166106421895	35.787239329227184	34.76703824239801
5	12.400914489242902	39.265384477645995	34.61189693736297	13.721804095748139
6	31.797114610473297	36.59822734810941	15.930257594695219	15.67440044672207
7	25.719545726451702	33.83106177400479	22.03877515740719	18.41061734213632
8	27.300448446354224	33.513395693447144	20.502571172013198	18.68358468818543
9	25.908155816870476	13.74647461390418	22.5137489505082	37.831620618717146
10	16.127754377137947	27.380296037375395	34.144085552758874	22.347864032727784
11	32.711161729394476	22.167035324379732	24.332779646734096	20.789023299491692
12	20.5238373355133	26.870306024956133	31.526667371117544	21.079189268413025
13	30.3910719251714	21.139716041451777	27.56673972028584	20.902472313090982
14	21.221951101971918	22.143646965769435	27.897050546708407	28.737351385550237
15	23.39025974341777	28.96128718207417	25.615956077635204	22.032496996872858
16	22.582985565978383	28.018015667989648	26.437201372883383	22.96179739314858
17	20.59762782799078	27.150170505113387	27.50484236290509	24.74735930399074
18	21.315725598403755	26.536944543062656	30.589718229965484	21.557611628568107
19	21.00774203303639	25.54512781583237	31.456855994471685	21.99027415665956
20	23.961528139643974	24.8318934092136	30.434134800949153	20.772443650193274
21	23.61932417812468	25.553970295458196	29.527515364913658	21.29919016150347
22	22.633034000660533	25.224057380618785	30.191099248433446	21.951809370287233
23	21.7913625774767	26.382908547980843	30.85225145005613	20.97347742448633
24	20.915647607733455	26.065375104617587	29.798670002639483	23.22030728500948
25	21.870546982525934	26.407136942155596	30.547274327761542	21.175041747556932
26	22.604428579071	25.99189409892701	29.592153890978416	21.811523431023573
27	21.754135738251993	26.869952325771095	29.32794059975887	22.047971336218044
28	21.479488321073973	26.58721403973545	29.48392194035836	22.44937569883222
29	20.806177709965784	26.47327868975674	30.27112368904713	22.449419911230347
30	20.521980414791877	26.871676609298135	31.24574179340519	21.3606011825048
31	21.41661829093438	26.347980753458845	30.63499172564969	21.600409229957084
32	22.090989999597667	26.507145386723636	29.262329400935265	22.13953521274343
33	22.564593208356673	26.35363994041937	29.136943039841118	21.944823811382836
34	21.563093965936115	27.926142304677366	29.18429451823739	21.326469211149128
35	20.729159712424877	28.446345381064447	29.524287859850233	21.30020704666044
36	21.28880024794313	26.26141287792205	30.66961003338478	21.780176840750038
37	21.3979606589239	26.247353335316998	30.15877998540107	22.19590602035804
38	21.389427666084977	25.98424535405067	30.66823944904278	21.95808753082157
39	21.963171956606413	25.5933193297931	30.719393193678158	21.72411551992233
40	20.813605392851475	25.936451751673108	30.40897794641369	22.84096490906173
41	21.24821326646061	25.519661474510002	30.513584480387156	22.71854077864223
42	19.687736674493735	26.748545080508567	30.71797839693803	22.845739848059672
43	20.043160143053633	26.329765245429655	30.552447178342646	23.074627433174065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	230.0
1	331.5
2	433.0
3	1133.5
4	1834.0
5	1834.0
6	2838.0
7	3842.0
8	4021.5
9	4201.0
10	6137.0
11	8073.0
12	8073.0
13	14122.0
14	20171.0
15	32096.5
16	44022.0
17	44072.5
18	44123.0
19	44123.0
20	47634.5
21	51146.0
22	43648.0
23	36150.0
24	40673.0
25	45196.0
26	45196.0
27	50601.0
28	56006.0
29	59895.0
30	63784.0
31	68532.5
32	73281.0
33	73281.0
34	80038.5
35	86796.0
36	94627.0
37	102458.0
38	107342.0
39	112226.0
40	112226.0
41	118075.5
42	123925.0
43	136491.5
44	149058.0
45	173524.0
46	197990.0
47	197990.0
48	200715.5
49	203441.0
50	188630.0
51	173819.0
52	165623.5
53	157428.0
54	157428.0
55	141697.5
56	125967.0
57	111002.5
58	96038.0
59	86846.0
60	77654.0
61	77654.0
62	69734.5
63	61815.0
64	54179.5
65	46544.0
66	40048.5
67	33553.0
68	33553.0
69	28247.5
70	22942.0
71	19476.0
72	16010.0
73	12775.5
74	9541.0
75	9541.0
76	7444.0
77	5347.0
78	4189.5
79	3032.0
80	2411.0
81	1790.0
82	1790.0
83	1331.0
84	872.0
85	695.0
86	518.0
87	414.5
88	311.0
89	311.0
90	249.0
91	187.0
92	108.5
93	30.0
94	25.0
95	20.0
96	20.0
97	12.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2261809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.66236400842493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72475290987461	36.84945490548353
2	13.393062431301441	13.034761584391028
3	4.609782073339215	6.7296867975703405
4	2.0621359464048283	4.013936402352384
5	1.0692417997804569	2.6015916836970003
6	0.6686656291296846	1.952331014677871
7	0.43203369345443926	1.4716646598348937
8	0.2986397488564053	1.1626012932988
9	0.21814637496562228	0.9553966475125921
>10	1.321992576876428	11.89191059101247
>50	0.12283169945081279	4.124949135985688
>100	0.06942592234350914	6.169747076965991
>500	0.004644597111290212	1.6129198932586877
>1k	0.004098173921726639	3.870697524305718
>5k	2.732115947817759E-4	0.9486955943120927
>10k+	2.732115947817759E-4	2.609655195340865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20712	0.9157271900500882	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19890	0.8793845987879613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18284	0.8083794873926137	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9298	0.4110868778044477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6991	0.30908887532059515	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5118	0.22627905362477557	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4791	0.21182159943655718	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4741	0.20961097953010177	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4625	0.20448234134712523	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4020	0.1777338404790148	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3777	0.16699022773364153	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3548	0.15686558856207575	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3398	0.15023372884270952	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3185	0.1408164880412095	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2940	0.129984450499578	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2656	0.11742812943091127	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2626	0.11610175748703803	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2601	0.11499644753381033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.842479625821632E-5	0.0	0.0	0.0	0.0
9	8.842479625821632E-5	0.0	0.0	0.0	0.0
10	8.842479625821632E-5	0.0	0.0	4.421239812910816E-5	0.0
11	8.842479625821632E-5	0.0	0.0	1.7684959251643265E-4	0.0
12	8.842479625821632E-5	0.0	0.0	3.0948678690375715E-4	0.0
13	8.842479625821632E-5	0.0	0.0	3.9791158316197344E-4	0.0
14	8.842479625821632E-5	0.0	0.0	4.863363794201898E-4	0.0
15	8.842479625821632E-5	0.0	0.0	8.842479625821632E-4	0.0
16	8.842479625821632E-5	0.0	0.0	0.0016800711289061102	0.0
17	8.842479625821632E-5	0.0	0.0	0.002254832304584516	0.0
18	8.842479625821632E-5	0.0	0.0	0.0026085314896173817	0.0
19	8.842479625821632E-5	0.0	0.0	0.0028738058783920307	0.0
20	8.842479625821632E-5	0.0	0.0	0.0036254166465868693	0.0
21	8.842479625821632E-5	0.0	0.0	0.005393912571751196	0.0
22	8.842479625821632E-5	0.0	0.0	0.008356143246401443	0.0
23	8.842479625821632E-5	0.0	0.0	0.014722728576993017	0.0
24	8.842479625821632E-5	0.0	0.0	0.023211509017781785	0.0
25	8.842479625821632E-5	0.0	0.0	0.02962230674650247	0.0
26	8.842479625821632E-5	0.0	0.0	0.038862697955486074	0.0
27	8.842479625821632E-5	0.0	0.0	0.11207842925728918	0.0
28	8.842479625821632E-5	0.0	0.0	0.18498467377218855	0.0
29	8.842479625821632E-5	0.0	0.0	0.27177361129962785	0.0
30	8.842479625821632E-5	0.0	0.0	0.42006199462465665	0.0
31	8.842479625821632E-5	0.0	0.0	0.6474021458045308	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4960	0.0	28.085686	1
TCTAACG	85	2.725004E-5	17.411764	2
GTATTGG	725	0.0	16.331036	1
GTATCAA	8600	0.0	16.176744	2
ATTGGAC	705	0.0	15.74468	3
CGTTTAG	155	7.219569E-9	15.5161295	26
TTGGACC	1045	0.0	15.401914	4
GTCTATA	145	5.3525582E-8	15.310345	1
TTACGCT	75	0.0041060387	14.8	4
CGCCCAA	100	1.0941071E-4	14.8	21
ACGCCTA	115	2.2116046E-5	14.47826	6
GTATACG	155	1.2116652E-7	14.322581	1
TAATACG	80	0.0063011055	13.875001	4
AATACGC	80	0.0063011055	13.875001	5
GGACCCT	1095	0.0	13.853881	6
TATACGG	175	3.5704943E-8	13.742857	2
GCGTGCG	245	7.2759576E-12	13.591836	9
CCCGTAT	110	2.458008E-4	13.454545	1
AATGCGT	165	2.5981353E-7	13.454544	35
TAACACT	235	5.638867E-11	13.3829775	4
>>END_MODULE