FastQCFastQC Report
Wed 25 May 2016
SRR2088381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284611
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213480.9344260357671395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206790.9051431512848358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187240.8195705964822896No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95340.4173139322186578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.3032463732337803No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53620.2347007871361908No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT51590.22581524819761437No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT49970.21872432549786375No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48580.21264013873696658No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41740.18270068733801947No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT40020.17517205336050645No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT37880.1658050320163914No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG36440.15950198961661308No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32110.1405490912895018No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30850.13503392918969576No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27670.12111471055685191No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT27670.12111471055685191No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.11962649221245981No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22860.10006079809648119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.027.3761651
GTATTGG6450.020.077521
ATTGGAC6700.019.6044773
CTAGCGG1002.878005E-718.529
TAGGTCG1156.413029E-817.69565221
ACGGTGT1554.0199666E-1016.70967929
CTGTGCG2350.016.5319149
TTGGACC10300.016.344664
GTATCAA83250.015.8666672
TGGACCC11400.015.4166665
CGCAATA1101.4530373E-515.13636436
GGACCCT11800.014.8940686
CCGTCGA750.00410605514.89
ACGGACC2006.184564E-1114.88
TATTGGA9400.014.1702122
ACGTTTA1601.785611E-713.87526
CCAACGG800.00630113513.87531
CGACGAG1356.5732947E-613.70370424
TAAACGT2457.2759576E-1213.5918364
GTCGTCA1501.3069985E-613.56666724
Produced by FastQC (version 0.11.5)