##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2284611
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.400968917684455	31.0	31.0	34.0	30.0	34.0
2	31.64007220485238	31.0	31.0	34.0	30.0	34.0
3	31.7301015358851	31.0	31.0	34.0	30.0	34.0
4	35.51527853100593	37.0	35.0	37.0	33.0	37.0
5	35.32531577585856	37.0	35.0	37.0	33.0	37.0
6	35.36788100906456	37.0	35.0	37.0	33.0	37.0
7	35.2433416454705	37.0	35.0	37.0	32.0	37.0
8	35.26110615767848	37.0	35.0	37.0	32.0	37.0
9	36.79411462170146	39.0	37.0	39.0	32.0	39.0
10	36.66032029085039	39.0	35.0	39.0	32.0	39.0
11	36.77673441999535	39.0	35.0	39.0	32.0	39.0
12	36.6372117616522	39.0	35.0	39.0	32.0	39.0
13	36.68770788549998	39.0	35.0	39.0	32.0	39.0
14	37.71967569096008	40.0	37.0	41.0	32.0	41.0
15	37.707442973880454	39.0	37.0	41.0	32.0	41.0
16	37.669825191247	39.0	36.0	41.0	32.0	41.0
17	37.71123092727821	39.0	37.0	41.0	32.0	41.0
18	37.6813299944717	39.0	37.0	41.0	32.0	41.0
19	37.74063505778445	40.0	37.0	41.0	32.0	41.0
20	37.70590660729551	39.0	37.0	41.0	32.0	41.0
21	37.65968385865252	39.0	37.0	41.0	32.0	41.0
22	37.56000124310003	39.0	36.0	41.0	32.0	41.0
23	37.46619621458533	39.0	36.0	41.0	32.0	41.0
24	37.391778293985276	39.0	36.0	41.0	32.0	41.0
25	37.3423961453394	39.0	36.0	41.0	31.0	41.0
26	37.12819293962955	39.0	36.0	41.0	31.0	41.0
27	36.988643580898454	39.0	36.0	40.0	31.0	41.0
28	36.89578138247605	39.0	35.0	40.0	31.0	41.0
29	36.75674545907378	39.0	35.0	40.0	30.0	41.0
30	36.59658909109691	38.0	35.0	40.0	30.0	41.0
31	36.377125908962185	38.0	35.0	40.0	30.0	41.0
32	36.244266091689134	38.0	35.0	40.0	30.0	41.0
33	36.32303179841119	38.0	35.0	40.0	30.0	41.0
34	36.29733552013888	38.0	35.0	40.0	30.0	41.0
35	36.246566264453776	38.0	35.0	40.0	30.0	41.0
36	36.17247137477671	38.0	35.0	40.0	30.0	41.0
37	36.07170892550198	38.0	35.0	40.0	29.0	41.0
38	35.96508683535184	38.0	35.0	40.0	29.0	41.0
39	35.92640760286981	38.0	35.0	40.0	28.0	41.0
40	35.78772491246869	38.0	35.0	40.0	27.0	41.0
41	35.66340921933756	38.0	35.0	40.0	27.0	41.0
42	35.54834192779427	38.0	35.0	40.0	26.0	41.0
43	35.22107964988351	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	15.0
17	42.0
18	132.0
19	256.0
20	594.0
21	1161.0
22	2185.0
23	3848.0
24	6440.0
25	10397.0
26	15783.0
27	23702.0
28	33673.0
29	46018.0
30	60613.0
31	76037.0
32	94435.0
33	117135.0
34	145950.0
35	180433.0
36	228662.0
37	309341.0
38	418020.0
39	509729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.161624451602485	21.96584013646087	13.392958363590127	23.479577048346524
2	17.898408087853905	22.808565659536786	36.07410627017028	23.218919982439022
3	19.534266446235268	25.78228853839888	31.83955605571364	22.84388895965221
4	13.189860330708377	16.099370965122727	36.011469786322486	34.69929891784641
5	12.226326494970042	39.41874568580822	34.805487673831564	13.549440145390179
6	31.69716857705754	36.740740546202396	15.960135007666512	15.601955869073553
7	25.481843517342778	34.04058721594179	22.12875627404403	18.3488129926714
8	27.066620969609268	33.78185607965645	20.542928314710906	18.608594636023376
9	25.87954798431768	13.79355172499826	22.63584478933175	37.69105550135231
10	15.996158645826359	27.48130863416135	34.324530521826254	22.198002198186035
11	32.5451466354666	22.28256801704973	24.32672345532784	20.84556189215582
12	20.34600201084561	27.06395093081492	31.67059950249736	20.919447555842112
13	30.387142493842497	21.28182872270159	27.513349099693556	20.817679683762353
14	21.17638407588863	22.234594861006972	28.0060369139429	28.5829841491615
15	23.263435219387457	29.12758452095346	25.63219734125416	21.97678291840493
16	22.32804621880924	28.251549169639823	26.5058252805401	22.914579331010838
17	20.3172881510244	27.309025475234076	27.614285320345566	24.759401053395962
18	21.16684197003341	26.625758170646996	30.795702200505907	21.41169765881369
19	20.87038012160495	25.58619388596133	31.743784828139233	21.79964116429449
20	23.82418713732885	24.8976740460411	30.60888702715692	20.66925178947313
21	23.499799309379146	25.579321818900457	29.744932507109525	21.17594636461087
22	22.32620783144264	25.411109374856377	30.42548600177448	21.8371967919265
23	21.610681205684468	26.578485352648656	30.99083388813238	20.819999553534497
24	20.71188486792719	26.230855055849773	29.962737638924086	23.094522437298952
25	21.65861059059945	26.607374296980975	30.688769335348553	21.04524577707102
26	22.40845378053419	26.090831218093584	29.87256911570504	21.628145885667188
27	21.502960460227147	26.98446256277327	29.582410309676355	21.930166667323235
28	21.36350564713205	26.6384517977021	29.728737189832316	22.26930536533353
29	20.620753379897057	26.663182484895675	30.48891036592225	22.227153769285014
30	20.315931246063336	27.035806095654795	31.515956108063907	21.132306550217958
31	21.320609941911336	26.552091362599583	30.687106032493062	21.44019266299602
32	21.813297756160676	26.762149004797752	29.49876368449596	21.92578955454561
33	22.397598540845685	26.487704033640735	29.30083064469181	21.813866780821765
34	21.31776481860588	28.219727559746495	29.45000264815323	21.012504973494394
35	20.542928314710906	28.7915098018875	29.639356546913238	21.026205336488356
36	21.139878955323248	26.575377602576545	30.757927717235013	21.526815724865195
37	21.149202205539584	26.66782222443996	30.24545535323081	21.93752021678964
38	21.25320240513593	26.357309843995324	30.734772790641387	21.654714960227363
39	21.768870061467798	25.91666590067193	30.8653420647979	21.449121973062375
40	20.71709363213256	26.31266329366356	30.498146073883035	22.472097000320844
41	21.15427965636163	25.823433398508545	30.5834997730467	22.438787172083124
42	19.508091311825076	27.001051820200463	30.92023105902931	22.570625808945156
43	19.91187996556088	26.640115100557598	30.69463466647057	22.75337026741095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	288.5
2	367.0
3	1009.5
4	1652.0
5	1652.0
6	2665.0
7	3678.0
8	3903.5
9	4129.0
10	6155.0
11	8181.0
12	8181.0
13	14357.5
14	20534.0
15	32964.0
16	45394.0
17	45101.0
18	44808.0
19	44808.0
20	48477.5
21	52147.0
22	44967.5
23	37788.0
24	42836.0
25	47884.0
26	47884.0
27	53698.0
28	59512.0
29	63926.5
30	68341.0
31	73566.0
32	78791.0
33	78791.0
34	85392.0
35	91993.0
36	99849.5
37	107706.0
38	112300.5
39	116895.0
40	116895.0
41	121786.0
42	126677.0
43	137409.0
44	148141.0
45	172226.5
46	196312.0
47	196312.0
48	199944.5
49	203577.0
50	188929.0
51	174281.0
52	165659.0
53	157037.0
54	157037.0
55	141767.5
56	126498.0
57	110654.5
58	94811.0
59	85377.0
60	75943.0
61	75943.0
62	67121.0
63	58299.0
64	51044.0
65	43789.0
66	37438.5
67	31088.0
68	31088.0
69	26508.5
70	21929.0
71	18697.5
72	15466.0
73	12381.5
74	9297.0
75	9297.0
76	7193.5
77	5090.0
78	3961.0
79	2832.0
80	2264.0
81	1696.0
82	1696.0
83	1239.0
84	782.0
85	619.5
86	457.0
87	380.5
88	304.0
89	304.0
90	263.0
91	222.0
92	131.5
93	41.0
94	34.0
95	27.0
96	27.0
97	16.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2284611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.342028334294966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79893252662254	36.159321155770044
2	13.861651052910767	13.40200655919844
3	4.800830472888725	6.962456482456004
4	2.2259077084666226	4.304195740488762
5	1.1437893697638617	2.764654906079499
6	0.6903153431109931	2.002274632576211
7	0.4458077260484116	1.5085874806995947
8	0.30727543058657014	1.1883454057478928
9	0.2196280042124416	0.9555536882378257
>10	1.3147175648432838	11.872697961870966
>50	0.11766603296971018	3.967159659213832
>100	0.06413902732361192	5.66778285579858
>500	0.004352500311918519	1.473343040415596
>1k	0.00435250031191848	3.9257878674701465
>5k	3.6270835932654004E-4	1.1828037029405185
>10k+	2.72031269494905E-4	2.6630288610361874
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21348	0.9344260357671395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20679	0.9051431512848358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18724	0.8195705964822896	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9534	0.4173139322186578	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6928	0.3032463732337803	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5362	0.2347007871361908	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5159	0.22581524819761437	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4997	0.21872432549786375	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4858	0.21264013873696658	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4174	0.18270068733801947	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4002	0.17517205336050645	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3788	0.1658050320163914	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3644	0.15950198961661308	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3211	0.1405490912895018	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3085	0.13503392918969576	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2767	0.12111471055685191	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2767	0.12111471055685191	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2733	0.11962649221245981	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2286	0.10006079809648119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.3771127776238495E-5	0.0
6	0.0	0.0	0.0	4.3771127776238495E-5	0.0
7	0.0	0.0	0.0	4.3771127776238495E-5	0.0
8	0.0	0.0	0.0	4.3771127776238495E-5	0.0
9	0.0	0.0	0.0	8.754225555247699E-5	0.0
10	0.0	0.0	0.0	1.3131338332871548E-4	0.0
11	0.0	0.0	0.0	1.3131338332871548E-4	0.0
12	0.0	0.0	0.0	3.0639789443366946E-4	0.0
13	0.0	0.0	0.0	3.5016902220990796E-4	0.0
14	0.0	0.0	0.0	3.5016902220990796E-4	0.0
15	0.0	0.0	0.0	6.565669166435774E-4	0.0
16	0.0	0.0	0.0	0.0010942781944059624	0.0
17	0.0	0.0	0.0	0.0013569049610633933	0.0
18	0.0	0.0	0.0	0.0015757605999445858	0.0
19	0.0	0.0	0.0	0.0018821584943782552	0.0
20	0.0	0.0	0.0	0.002538725411021833	0.0
21	0.0	0.0	0.0	0.003545461349875318	0.0
22	0.0	0.0	0.0	0.006171729016449627	0.0
23	0.0	0.0	0.0	0.010636384049625953	0.0
24	0.0	0.0	0.0	0.017552222238271634	0.0
25	0.0	0.0	0.0	0.0214916237381331	0.0
26	0.0	0.0	0.0	0.030289620421157036	0.0
27	0.0	0.0	0.0	0.09581499870218606	0.0
28	0.0	0.0	0.0	0.1653235496108528	0.0
29	0.0	0.0	0.0	0.2450307732913831	0.0
30	0.0	0.0	0.0	0.3802397869921838	0.0
31	0.0	0.0	0.0	0.5886778974626314	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4825	0.0	27.376165	1
GTATTGG	645	0.0	20.07752	1
ATTGGAC	670	0.0	19.604477	3
CTAGCGG	100	2.878005E-7	18.5	29
TAGGTCG	115	6.413029E-8	17.695652	21
ACGGTGT	155	4.0199666E-10	16.709679	29
CTGTGCG	235	0.0	16.531914	9
TTGGACC	1030	0.0	16.34466	4
GTATCAA	8325	0.0	15.866667	2
TGGACCC	1140	0.0	15.416666	5
CGCAATA	110	1.4530373E-5	15.136364	36
GGACCCT	1180	0.0	14.894068	6
CCGTCGA	75	0.004106055	14.8	9
ACGGACC	200	6.184564E-11	14.8	8
TATTGGA	940	0.0	14.170212	2
ACGTTTA	160	1.785611E-7	13.875	26
CCAACGG	80	0.006301135	13.875	31
CGACGAG	135	6.5732947E-6	13.703704	24
TAAACGT	245	7.2759576E-12	13.591836	4
GTCGTCA	150	1.3069985E-6	13.566667	24
>>END_MODULE