FastQCFastQC Report
Wed 25 May 2016
SRR2088380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963192
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158171.6421440377411771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148581.5425792573028014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138041.4331514381348682No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG85380.8864276281364464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.5074792980008139No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT45530.4726991087965847No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT44550.4625246056860937No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44290.45982524771800426No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40130.4166355202285733No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37670.3910954409920348No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35650.37012350600918614No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG31350.3254802780753993No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT29240.3035739499497504No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27740.2880007309030806No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25930.2692090465867657No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA24060.24979443350858394No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22700.23567471490626998No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.20224420468608542No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA19090.19819516773395127No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18060.18750155732190468No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16350.16974808760870108No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15870.16476465751376673No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15790.16393408583127766No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15330.1591582986569656No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15180.15760097675229862No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG12310.12780421764300368No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.12697364596051464No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.12136728710371349No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC11190.11617621408815687No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA10640.11046603377104461No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA10560.10963546208855555No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC10410.10807814018388857No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA10120.10506731783486575No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG9880.10257560278739858No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG9870.10247178132708744No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC9810.10184885256522064No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG9650.10018770920024252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC250.005495907729.59999810
GGTATCA37300.028.9651491
ATTGGAC3050.025.4754093
GTATTGG3300.022.9848481
CGCTCTA502.7016623E-422.19999914
TATACCG705.1005045E-621.1428595
ACCCGTG450.003825365320.55555516
TATTGGA3900.019.9230772
TTGGACC6250.019.5364
CGACGAG801.6162277E-518.524
CATCGGG701.2189367E-418.521
CGGTTTA1002.8737304E-718.49999815
TCTATGC500.00703407618.4999983
GTATCAA63100.017.1220282
TGGACCC7250.017.0965525
TAGGTCG650.001579682217.0769235
GACCCTC6950.017.035977
GGACCCT7100.016.936626
TGTTCTA803.3818546E-416.18755
ATAGAAC700.00259208415.8571433
Produced by FastQC (version 0.11.5)