##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963192
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339947798569757	31.0	31.0	34.0	30.0	34.0
2	31.558685080440867	31.0	31.0	34.0	30.0	34.0
3	31.637675562089385	31.0	31.0	34.0	30.0	34.0
4	35.447664640071764	37.0	35.0	37.0	33.0	37.0
5	35.29178294670221	37.0	35.0	37.0	33.0	37.0
6	35.353475734848296	37.0	35.0	37.0	32.0	37.0
7	35.196011802423605	37.0	35.0	37.0	32.0	37.0
8	35.24164237244496	37.0	35.0	37.0	32.0	37.0
9	36.70368732298441	39.0	35.0	39.0	32.0	39.0
10	36.615695520726916	39.0	35.0	39.0	32.0	39.0
11	36.732143747041086	39.0	35.0	39.0	32.0	39.0
12	36.58879745678951	39.0	35.0	39.0	32.0	39.0
13	36.61168282128589	39.0	35.0	39.0	32.0	39.0
14	37.55458205632937	39.0	36.0	41.0	32.0	41.0
15	37.53532836651467	39.0	36.0	41.0	32.0	41.0
16	37.49534153107584	39.0	36.0	41.0	32.0	41.0
17	37.61363570295434	39.0	36.0	41.0	32.0	41.0
18	37.620212792465054	39.0	36.0	41.0	32.0	41.0
19	37.71654353441474	39.0	37.0	41.0	32.0	41.0
20	37.68900489206721	39.0	37.0	41.0	32.0	41.0
21	37.64088883628602	39.0	37.0	41.0	32.0	41.0
22	37.54187223315808	39.0	36.0	41.0	32.0	41.0
23	37.40693651940631	39.0	36.0	41.0	32.0	41.0
24	37.33400817282536	39.0	36.0	41.0	31.0	41.0
25	37.281846194735834	39.0	36.0	41.0	31.0	41.0
26	37.03345854201447	39.0	36.0	41.0	31.0	41.0
27	36.88929829151405	39.0	35.0	40.0	31.0	41.0
28	36.768196787348735	39.0	35.0	40.0	30.0	41.0
29	36.59864388408542	38.0	35.0	40.0	30.0	41.0
30	36.39926722813313	38.0	35.0	40.0	30.0	41.0
31	36.088122617297486	38.0	35.0	40.0	30.0	41.0
32	35.93021225259346	38.0	35.0	40.0	30.0	41.0
33	35.91786788096247	38.0	35.0	40.0	30.0	41.0
34	35.82475975714084	38.0	35.0	40.0	29.0	41.0
35	35.71017927889766	38.0	35.0	40.0	29.0	41.0
36	35.61422852349272	38.0	35.0	40.0	27.0	41.0
37	35.465794981685896	38.0	34.0	40.0	27.0	41.0
38	35.316985606192745	38.0	34.0	40.0	26.0	41.0
39	35.20938608294089	38.0	34.0	40.0	25.0	41.0
40	34.97405190242444	38.0	34.0	40.0	24.0	41.0
41	34.77795704283258	38.0	33.0	40.0	23.0	41.0
42	34.55908686949227	38.0	33.0	40.0	23.0	41.0
43	34.21179577903471	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	9.0
17	17.0
18	47.0
19	127.0
20	311.0
21	567.0
22	1077.0
23	1888.0
24	3147.0
25	5074.0
26	7805.0
27	11587.0
28	16421.0
29	22326.0
30	28638.0
31	34849.0
32	42574.0
33	52265.0
34	64067.0
35	78413.0
36	99351.0
37	137370.0
38	168557.0
39	186701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74754358424904	22.431872357743835	14.18170001411972	21.63888404388741
2	17.79385626126463	22.764723959501325	36.73639315941163	22.705026619822423
3	19.93548534456266	26.01724266812847	32.93860414123041	21.108667846078454
4	12.445182268955723	15.369832805920314	34.8774699125408	37.30751501258316
5	11.124054186496565	40.684619473583666	34.24436664756352	13.946959692356248
6	31.18775903454348	35.45855862590221	15.437628219503486	17.91605412005083
7	24.37541009476823	36.04463076935855	20.701376257277886	18.87858287859534
8	28.718469422503507	32.511690296431034	19.963101853005423	18.806738428060033
9	25.58856385850381	13.3710620520104	23.856198971752256	37.18417511773354
10	16.70549589282303	27.447694748295255	32.931544281929256	22.915265076952466
11	30.92633659748005	21.416809940281897	25.647742090881152	22.009111371356905
12	20.869151737140673	27.49815197800646	31.423641392370367	20.209054892482495
13	31.947835945481273	20.61582737398151	29.110499256638345	18.325837423898868
14	21.126109851410728	22.443708004219303	28.718469422503507	27.711712721866462
15	23.262236397312268	28.332253590146095	27.63446955539498	20.771040457146654
16	21.68591516540835	28.254283673452434	27.352490469189945	22.707310691949267
17	19.056948147409862	26.670072010564873	27.800480070432478	26.47249977159279
18	20.42946785272303	25.868674158423243	33.24986087924318	20.451997109610545
19	19.486146064336083	24.013384662663313	34.66027541756991	21.84019385543069
20	25.031354081013962	22.513060739707143	33.23989401905331	19.215691160225585
21	24.22300019103149	23.909147916510936	31.813179511457733	20.05467238099984
22	21.990838794342146	23.34093306422811	32.97006204370469	21.698166097725064
23	20.698053970547928	25.635491158564438	34.4382013139644	19.22825355692323
24	19.077504796551466	24.560523758502978	32.51023679598668	23.851734648958878
25	20.549381639382386	25.820604822299188	33.851921527587436	19.778092010730987
26	22.462084402694373	24.452964725620642	32.059755479696676	21.025195391988305
27	20.617696160267112	26.437511939467935	31.482923446208027	21.461868454056926
28	20.204279105308185	25.713668718178724	31.609170341946363	22.47288183456673
29	19.09058630055067	25.76485269811211	32.96777797157784	22.176783029759385
30	18.36352461399181	26.0389413533335	35.32172194121214	20.27581209146255
31	20.21154660752996	25.332332494455933	33.67002632912233	20.78609456889177
32	21.68404637912275	25.538937200475086	31.277564597712605	21.499451822689558
33	22.900730072508907	25.312710238457132	30.656089336290172	21.130470352743792
34	20.447948072658413	29.021731908072322	30.769462370949924	19.760857648319337
35	18.75202451847607	30.136670570353573	31.117160441531905	19.994144469638453
36	19.94877449148249	25.64826119818271	33.739690528991105	20.6632737813437
37	20.31391456739674	26.137467919168767	32.372881003995055	21.175736509439446
38	20.516158772082825	25.538833379014775	33.108248407378795	20.8367594415236
39	21.962391714216896	24.923379762290384	32.77736941336722	20.3368591101255
40	19.736148140765287	25.964501366290417	31.688282294703445	22.61106819824085
41	21.043571790463375	25.369292934326698	31.547188930140617	22.03994634506931
42	18.041158979725747	27.440531067533787	32.31391041453833	22.204399538202143
43	18.953438151479663	27.25126454538659	31.5778162609324	22.21748104220135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35.0
1	80.5
2	126.0
3	425.0
4	724.0
5	724.0
6	1311.0
7	1898.0
8	2098.5
9	2299.0
10	3574.5
11	4850.0
12	4850.0
13	8618.0
14	12386.0
15	21098.5
16	29811.0
17	28997.0
18	28183.0
19	28183.0
20	29417.0
21	30651.0
22	23575.5
23	16500.0
24	17921.5
25	19343.0
26	19343.0
27	20264.0
28	21185.0
29	21476.5
30	21768.0
31	23214.0
32	24660.0
33	24660.0
34	26752.5
35	28845.0
36	32014.5
37	35184.0
38	36940.5
39	38697.0
40	38697.0
41	41744.5
42	44792.0
43	53052.5
44	61313.0
45	82718.0
46	104123.0
47	104123.0
48	105670.5
49	107218.0
50	96402.0
51	85586.0
52	79763.0
53	73940.0
54	73940.0
55	63337.5
56	52735.0
57	42471.5
58	32208.0
59	27982.5
60	23757.0
61	23757.0
62	21181.5
63	18606.0
64	16390.0
65	14174.0
66	12191.5
67	10209.0
68	10209.0
69	8544.5
70	6880.0
71	5772.0
72	4664.0
73	3666.5
74	2669.0
75	2669.0
76	2039.0
77	1409.0
78	1089.5
79	770.0
80	606.0
81	442.0
82	442.0
83	328.5
84	215.0
85	184.5
86	154.0
87	129.0
88	104.0
89	104.0
90	80.5
91	57.0
92	35.0
93	13.0
94	10.0
95	7.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	963192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.784894758104194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60619993974306	36.90546381091841
2	10.820060423132443	9.907906554988944
3	3.3533877553898854	4.606035163909235
4	1.448421415281305	2.6526328825615555
5	0.7932206830575164	1.8158762746869956
6	0.5272449843138384	1.4483913671128434
7	0.3490490307074034	1.118682119544972
8	0.2604983218022801	0.9541510598704124
9	0.22029580099039445	0.9077598057597728
>10	1.3953032529820195	12.385304436939636
>50	0.13000847496373377	4.136039076714618
>100	0.07786759298807591	6.708024907764923
>500	0.010928784976599031	3.8173268278486696
>1k	0.006602807590028581	7.109678699935415
>5k	2.2768302034581318E-4	0.8900389539903313
>10k+	6.830490610374395E-4	4.636688057453261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15817	1.6421440377411771	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14858	1.5425792573028014	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13804	1.4331514381348682	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8538	0.8864276281364464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4888	0.5074792980008139	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4553	0.4726991087965847	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4455	0.4625246056860937	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4429	0.45982524771800426	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4013	0.4166355202285733	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3767	0.3910954409920348	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3565	0.37012350600918614	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3135	0.3254802780753993	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2924	0.3035739499497504	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2774	0.2880007309030806	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2593	0.2692090465867657	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2406	0.24979443350858394	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2270	0.23567471490626998	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1948	0.20224420468608542	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1909	0.19819516773395127	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1806	0.18750155732190468	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1635	0.16974808760870108	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1587	0.16476465751376673	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1579	0.16393408583127766	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1533	0.1591582986569656	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1518	0.15760097675229862	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1231	0.12780421764300368	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1223	0.12697364596051464	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1169	0.12136728710371349	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1119	0.11617621408815687	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1064	0.11046603377104461	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1056	0.10963546208855555	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1041	0.10807814018388857	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1012	0.10506731783486575	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	988	0.10257560278739858	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	987	0.10247178132708744	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	981	0.10184885256522064	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	965	0.10018770920024252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0382146031113215E-4	0.0
8	1.0382146031113215E-4	0.0	0.0	1.0382146031113215E-4	0.0
9	1.0382146031113215E-4	0.0	0.0	1.0382146031113215E-4	0.0
10	1.0382146031113215E-4	0.0	0.0	3.1146438093339644E-4	0.0
11	1.0382146031113215E-4	0.0	0.0	8.305716824890572E-4	0.0
12	1.0382146031113215E-4	0.0	0.0	0.0012458575237335858	0.0
13	1.0382146031113215E-4	0.0	0.0	0.0012458575237335858	0.0
14	1.0382146031113215E-4	0.0	0.0	0.001972607745911511	0.0
15	2.076429206222643E-4	0.0	0.0	0.0030108223490228326	0.0
16	3.1146438093339644E-4	0.0	0.0	0.004983430094934343	0.0
17	4.152858412445286E-4	0.0	0.0	0.007267502221779251	0.0
18	4.152858412445286E-4	0.0	0.0	0.007786609523334912	0.0
19	4.152858412445286E-4	0.0	0.0	0.009240109967690762	0.0
20	4.152858412445286E-4	0.0	0.0	0.011420360634224537	0.0
21	4.152858412445286E-4	0.0	0.0	0.015261754665736427	0.0
22	4.152858412445286E-4	0.0	0.0	0.025332436315916244	0.0
23	4.152858412445286E-4	0.0	0.0	0.044331763552853426	0.0
24	4.152858412445286E-4	0.0	0.0	0.0648884126944576	0.0
25	4.152858412445286E-4	0.0	0.0	0.07599730894774874	0.0
26	4.152858412445286E-4	0.0	0.0	0.08606799059792855	0.0
27	4.152858412445286E-4	0.0	0.0	0.1678793013231007	0.0
28	4.152858412445286E-4	0.0	0.0	0.2515593983338732	0.0
29	4.152858412445286E-4	0.0	0.0	0.3551732157243831	0.0
30	4.152858412445286E-4	0.0	0.0	0.5417403799034876	0.0
31	4.152858412445286E-4	0.0	0.0	0.7708743428101562	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	25	0.0054959077	29.599998	10
GGTATCA	3730	0.0	28.965149	1
ATTGGAC	305	0.0	25.475409	3
GTATTGG	330	0.0	22.984848	1
CGCTCTA	50	2.7016623E-4	22.199999	14
TATACCG	70	5.1005045E-6	21.142859	5
ACCCGTG	45	0.0038253653	20.555555	16
TATTGGA	390	0.0	19.923077	2
TTGGACC	625	0.0	19.536	4
CGACGAG	80	1.6162277E-5	18.5	24
CATCGGG	70	1.2189367E-4	18.5	21
CGGTTTA	100	2.8737304E-7	18.499998	15
TCTATGC	50	0.007034076	18.499998	3
GTATCAA	6310	0.0	17.122028	2
TGGACCC	725	0.0	17.096552	5
TAGGTCG	65	0.0015796822	17.076923	5
GACCCTC	695	0.0	17.03597	7
GGACCCT	710	0.0	16.93662	6
TGTTCTA	80	3.3818546E-4	16.1875	5
ATAGAAC	70	0.002592084	15.857143	3
>>END_MODULE