FastQCFastQC Report
Wed 25 May 2016
SRR2088379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792483
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77980.9839958712048081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73510.9275908757664202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67960.8575578277388916No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32440.40934632036270807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.3211425355496585No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT16600.20946821572197763No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16330.20606120257469243No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT16110.20328511778801564No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14940.18852139414977986No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT13720.17312674215093574No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA13620.171864885429719No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT12750.16088673195513342No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG11780.1486467217593311No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9950.1255547437610649No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.12404051569560481No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9710.12252628763014474No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9290.11722648940103446No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9130.1152075186470877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.027.648351
CGCAATA509.086434E-625.90000236
GTATTGG1700.023.9411771
CGACGAG400.001930660723.12524
ATTGGAC1850.023.0000023
CTAGCGG603.7244077E-521.58333229
TTGGACC3100.020.2903214
TCTAGCG656.8986745E-519.92307728
GCAATAC759.259818E-619.73333437
GTATAAC500.007033000218.51
TAGGAAC2000.018.537
GTAACAC701.2185912E-418.53
GGACCCT2950.018.1864416
CGTCTCT752.0662458E-417.26666610
AAGCGTG650.001579348917.0769237
GACCCTC3000.016.657
TCTTGCG904.443153E-516.4444452
TGGACCC3600.016.4444455
GTATTAG1251.6556805E-716.2799991
TTAAGGG700.002591541315.8571423
Produced by FastQC (version 0.11.5)