##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792483
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4563605780818	31.0	31.0	34.0	30.0	34.0
2	31.66229559498437	31.0	31.0	34.0	30.0	34.0
3	31.749794001890262	31.0	31.0	34.0	30.0	34.0
4	35.528594304231135	37.0	35.0	37.0	33.0	37.0
5	35.347988537293546	37.0	35.0	37.0	33.0	37.0
6	35.3752850218869	37.0	35.0	37.0	33.0	37.0
7	35.259493263577895	37.0	35.0	37.0	32.0	37.0
8	35.277850755158155	37.0	35.0	37.0	32.0	37.0
9	36.801497319185394	39.0	37.0	39.0	32.0	39.0
10	36.66734933115284	39.0	35.0	39.0	32.0	39.0
11	36.77669426347316	39.0	37.0	39.0	32.0	39.0
12	36.637338592752144	39.0	35.0	39.0	32.0	39.0
13	36.66723828776138	39.0	35.0	39.0	32.0	39.0
14	37.70681894753578	40.0	37.0	41.0	32.0	41.0
15	37.70336146011965	40.0	37.0	41.0	32.0	41.0
16	37.66033088406944	39.0	36.0	41.0	32.0	41.0
17	37.705881387991916	39.0	37.0	41.0	32.0	41.0
18	37.683406457930325	39.0	37.0	41.0	32.0	41.0
19	37.74000451744706	40.0	37.0	41.0	32.0	41.0
20	37.7051608678041	40.0	37.0	41.0	32.0	41.0
21	37.6464176518613	39.0	37.0	41.0	32.0	41.0
22	37.54655557280093	39.0	36.0	41.0	32.0	41.0
23	37.43642576560002	39.0	36.0	41.0	32.0	41.0
24	37.37187043759929	39.0	36.0	41.0	31.0	41.0
25	37.31016059650491	39.0	36.0	41.0	31.0	41.0
26	37.10175107857203	39.0	36.0	41.0	31.0	41.0
27	36.93738666949323	39.0	35.0	41.0	31.0	41.0
28	36.85584044074132	39.0	35.0	40.0	31.0	41.0
29	36.70699308376331	39.0	35.0	40.0	30.0	41.0
30	36.52556963367037	38.0	35.0	40.0	30.0	41.0
31	36.32003588720515	38.0	35.0	40.0	30.0	41.0
32	36.164607947426	38.0	35.0	40.0	30.0	41.0
33	36.2508104274792	38.0	35.0	40.0	30.0	41.0
34	36.22750393383833	38.0	35.0	40.0	30.0	41.0
35	36.167684354112325	38.0	35.0	40.0	30.0	41.0
36	36.08616967177845	38.0	35.0	40.0	29.0	41.0
37	35.97019999167175	38.0	35.0	40.0	29.0	41.0
38	35.86491066685342	38.0	35.0	40.0	28.0	41.0
39	35.83309168777122	38.0	35.0	40.0	28.0	41.0
40	35.69954812910813	38.0	35.0	40.0	27.0	41.0
41	35.58264467502773	38.0	35.0	40.0	27.0	41.0
42	35.43103258997354	38.0	34.0	40.0	26.0	41.0
43	35.106027511000235	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	5.0
17	12.0
18	45.0
19	119.0
20	232.0
21	426.0
22	770.0
23	1428.0
24	2362.0
25	3736.0
26	5938.0
27	8398.0
28	12184.0
29	16683.0
30	21437.0
31	26491.0
32	32831.0
33	40834.0
34	50406.0
35	61535.0
36	78388.0
37	107234.0
38	143727.0
39	177258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26195766975443	22.008800188773765	13.387921255093168	23.341320886378636
2	17.961394755471094	22.977779964996095	35.93553426382648	23.12529101570633
3	19.472720550472374	25.97645627729554	31.803205873185924	22.747617299046162
4	13.073088003149595	16.23656280323994	36.39497629602149	34.29537289758897
5	12.147516098137121	39.57548616184827	34.791534960371386	13.485462779643223
6	31.289125444962224	37.01215041836859	16.11731734308496	15.581406793584215
7	25.367484223636342	34.2175163378899	22.311014873505172	18.103984564968588
8	27.01357631646357	33.752143579105166	20.733315414967894	18.50096468946337
9	25.820238415208905	13.930645830888485	22.833802113105264	37.41531364079734
10	16.158832429212993	27.548477380587343	34.22256376477477	22.0701264254249
11	32.3474446770467	22.516571333391376	24.396611662332187	20.73937232722973
12	20.263904714675267	27.363615370929097	31.6703323604418	20.70214755395384
13	29.979191982667135	21.313517135383346	27.93346986623057	20.773821015718948
14	21.043479797042966	22.437579102643213	28.222182684044956	28.29675841626887
15	23.23254883700975	29.178165336038752	25.914499112283796	21.674786714667697
16	22.309753016783958	28.398464067998937	26.61836279137849	22.67342012383862
17	20.233746339038188	27.469358964167057	27.93586739400088	24.36102730279388
18	21.03855855583022	26.727765769107982	30.927982051349996	21.305693623711804
19	20.74050799827883	25.75071010986986	31.976711172353223	21.532070719498083
20	23.589528103441967	24.98943194995981	30.97429219301865	20.446747753579572
21	23.31986932211795	25.739605770723156	29.952692991521584	20.987831915637305
22	22.253978949706177	25.56572191453949	30.796622766671334	21.383676369082995
23	21.434781566292273	26.582021317807445	31.370136646464342	20.613060469435936
24	20.577980852586112	26.237282061571037	30.34727558824606	22.837461497596795
25	21.483110678714876	26.761962086252954	31.006469539409675	20.748457695622495
26	22.297765377932397	26.27526394887966	29.923544101261477	21.503426571926465
27	21.397052050327893	27.107458456522092	29.80972462500773	21.685764868142282
28	21.13748812277361	26.739753407959537	30.043168118432824	22.079590350834025
29	20.38315017483025	26.745684134549258	30.892019134795323	21.97914655582517
30	20.182514956156787	27.074019253409855	31.769261927385195	20.974203863048167
31	21.268847407452274	26.760574043859613	30.837506924438756	21.133071624249354
32	21.7714449395129	26.76625239910509	29.748524574028718	21.713778087353294
33	22.124638635781462	26.695714608389075	29.68946967947577	21.49017707635369
34	21.324747660202174	28.099908767759057	29.702214432360062	20.873129139678706
35	20.55955774445635	28.621560336310054	29.86259642162671	20.95628549760689
36	20.988462843997915	26.687260168356925	30.915742041154193	21.408534946490963
37	21.116919858217777	26.568771822234673	30.439769685911244	21.874538633636305
38	20.943162187706235	26.431103253949928	31.098080337370014	21.527654220973822
39	21.568412193069125	25.91109209913651	31.09227579645242	21.428219911341948
40	20.56586702806243	26.215325754621865	30.77554975942702	22.443257457888688
41	21.01470946379922	25.925729637102624	30.74362478438023	22.315936114717918
42	19.480796433488162	27.08474503554019	31.006469539409675	22.427988991561964
43	19.738089018944255	26.62997187321368	30.855425289880035	22.776513817962027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	112.0
2	160.0
3	388.5
4	617.0
5	617.0
6	993.5
7	1370.0
8	1473.5
9	1577.0
10	2290.0
11	3003.0
12	3003.0
13	5167.5
14	7332.0
15	11683.5
16	16035.0
17	15930.0
18	15825.0
19	15825.0
20	17356.0
21	18887.0
22	16066.0
23	13245.0
24	15183.5
25	17122.0
26	17122.0
27	18938.0
28	20754.0
29	22158.0
30	23562.0
31	25571.0
32	27580.0
33	27580.0
34	30301.5
35	33023.0
36	36029.0
37	39035.0
38	40929.0
39	42823.0
40	42823.0
41	44444.0
42	46065.0
43	49219.5
44	52374.0
45	60511.0
46	68648.0
47	68648.0
48	68877.5
49	69107.0
50	63316.0
51	57525.0
52	54645.0
53	51765.0
54	51765.0
55	46747.0
56	41729.0
57	37106.0
58	32483.0
59	29268.0
60	26053.0
61	26053.0
62	23061.5
63	20070.0
64	17577.5
65	15085.0
66	12776.5
67	10468.0
68	10468.0
69	8907.5
70	7347.0
71	6147.0
72	4947.0
73	3920.5
74	2894.0
75	2894.0
76	2241.5
77	1589.0
78	1247.5
79	906.0
80	760.5
81	615.0
82	615.0
83	451.5
84	288.0
85	257.5
86	227.0
87	189.5
88	152.0
89	152.0
90	125.5
91	99.0
92	61.0
93	23.0
94	15.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	792483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.03087315656239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.65225286105856	49.38065527901831
2	9.726402033801968	11.48316009454189
3	2.726560256170667	4.828536979072048
4	1.1775941538127375	2.780576444945165
5	0.6012390156755294	1.7745832035559306
6	0.39943368414031394	1.4147351485767168
7	0.26294955716686597	1.0865499368984062
8	0.19799747929566525	0.9350371268497203
9	0.15603448846918808	0.8289766887186365
>10	0.9650428547704951	10.845704592206177
>50	0.08430023836364738	3.416048858718294
>100	0.044401833745471166	4.946368630298833
>500	0.0030030225705795794	1.3004097332166562
>1k	0.002145016121842557	2.199933661626698
>5k	6.435048365527671E-4	2.778723621756526
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7798	0.9839958712048081	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7351	0.9275908757664202	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6796	0.8575578277388916	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3244	0.40934632036270807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2545	0.3211425355496585	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1660	0.20946821572197763	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1633	0.20606120257469243	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1611	0.20328511778801564	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1494	0.18852139414977986	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1372	0.17312674215093574	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1362	0.171864885429719	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1275	0.16088673195513342	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1178	0.1486467217593311	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	995	0.1255547437610649	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.12404051569560481	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	971	0.12252628763014474	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	929	0.11722648940103446	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	913	0.1152075186470877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.2618567212167327E-4	0.0
6	1.2618567212167327E-4	0.0	0.0	1.2618567212167327E-4	0.0
7	1.2618567212167327E-4	0.0	0.0	1.2618567212167327E-4	0.0
8	1.2618567212167327E-4	0.0	0.0	1.2618567212167327E-4	0.0
9	1.2618567212167327E-4	0.0	0.0	1.2618567212167327E-4	0.0
10	1.2618567212167327E-4	0.0	0.0	1.2618567212167327E-4	0.0
11	1.2618567212167327E-4	0.0	0.0	2.5237134424334654E-4	0.0
12	1.2618567212167327E-4	0.0	0.0	7.571140327300396E-4	0.0
13	1.2618567212167327E-4	0.0	0.0	7.571140327300396E-4	0.0
14	2.5237134424334654E-4	0.0	0.0	7.571140327300396E-4	0.0
15	2.5237134424334654E-4	0.0	0.0	0.0010094853769733862	0.0
16	3.785570163650198E-4	0.0	0.0	0.0021451564260684458	0.0
17	3.785570163650198E-4	0.0	0.0	0.0030284561309201585	0.0
18	3.785570163650198E-4	0.0	0.0	0.0034070131472851784	1.2618567212167327E-4
19	3.785570163650198E-4	0.0	0.0	0.004668869868501911	1.2618567212167327E-4
20	3.785570163650198E-4	0.0	0.0	0.005425983901231951	1.2618567212167327E-4
21	3.785570163650198E-4	0.0	0.0	0.00769732599942207	1.2618567212167327E-4
22	3.785570163650198E-4	0.0	0.0	0.011735267507315614	1.2618567212167327E-4
23	3.785570163650198E-4	0.0	0.0	0.016656508720060872	1.2618567212167327E-4
24	3.785570163650198E-4	0.0	0.0	0.025489505768578002	1.2618567212167327E-4
25	3.785570163650198E-4	0.0	0.0	0.03003218996495824	1.2618567212167327E-4
26	3.785570163650198E-4	0.0	0.0	0.0387390013413537	1.2618567212167327E-4
27	3.785570163650198E-4	0.0	0.0	0.1029675084512854	1.2618567212167327E-4
28	3.785570163650198E-4	0.0	0.0	0.15684879044723987	1.2618567212167327E-4
29	3.785570163650198E-4	0.0	0.0	0.23495772149055563	1.2618567212167327E-4
30	3.785570163650198E-4	0.0	0.0	0.36783123423467756	1.2618567212167327E-4
31	3.785570163650198E-4	0.0	0.0	0.5849967759560772	1.2618567212167327E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1820	0.0	27.64835	1
CGCAATA	50	9.086434E-6	25.900002	36
GTATTGG	170	0.0	23.941177	1
CGACGAG	40	0.0019306607	23.125	24
ATTGGAC	185	0.0	23.000002	3
CTAGCGG	60	3.7244077E-5	21.583332	29
TTGGACC	310	0.0	20.290321	4
TCTAGCG	65	6.8986745E-5	19.923077	28
GCAATAC	75	9.259818E-6	19.733334	37
GTATAAC	50	0.0070330002	18.5	1
TAGGAAC	200	0.0	18.5	37
GTAACAC	70	1.2185912E-4	18.5	3
GGACCCT	295	0.0	18.186441	6
CGTCTCT	75	2.0662458E-4	17.266666	10
AAGCGTG	65	0.0015793489	17.076923	7
GACCCTC	300	0.0	16.65	7
TCTTGCG	90	4.443153E-5	16.444445	2
TGGACCC	360	0.0	16.444445	5
GTATTAG	125	1.6556805E-7	16.279999	1
TTAAGGG	70	0.0025915413	15.857142	3
>>END_MODULE