FastQCFastQC Report
Wed 25 May 2016
SRR2088378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286087
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136430.5967839369192861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136190.5957341081069968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116520.5096918883664533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52840.23113731017236003No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30810.13477177377763838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA706.5992936E-926.4285714
GGTATCA39750.021.5949691
TATACCG1653.6379788E-1217.9393945
GTATTGG2600.017.0769231
CGGGATA1452.9849616E-916.58620624
ACGTTTA1455.3525582E-815.31034526
GTACTAG2006.184564E-1114.7999991
CCGTCGT1406.0018283E-714.5357149
TACGTGC1406.0018283E-714.5357148
ATACCGA2450.014.346946
ATAGACG1051.6567612E-414.0952393
ACTACGT1601.785611E-713.8750016
TGCGACG1356.5733E-613.70370422
CAATGCG1356.5733E-613.70370419
GCGCGAT1501.3070003E-613.56666613
GTATCAA63300.013.56082252
TACCGAC2102.044544E-913.2142877
TACTCCG1703.7356585E-713.0588245
GCGCTAG850.00940845113.05882411
ATACCGC1857.392555E-813.00000127
Produced by FastQC (version 0.11.5)