##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2286087
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44033888474061	31.0	31.0	34.0	30.0	34.0
2	31.65221489820816	31.0	31.0	34.0	30.0	34.0
3	31.751268871219686	31.0	31.0	34.0	30.0	34.0
4	35.52459902007229	37.0	35.0	37.0	33.0	37.0
5	35.3264622037569	37.0	35.0	37.0	33.0	37.0
6	35.341000583092416	37.0	35.0	37.0	32.0	37.0
7	35.23952150552451	37.0	35.0	37.0	32.0	37.0
8	35.248267016959545	37.0	35.0	37.0	32.0	37.0
9	36.81536616935401	39.0	37.0	39.0	32.0	39.0
10	36.6411037725161	39.0	35.0	39.0	32.0	39.0
11	36.74528528441831	39.0	35.0	39.0	32.0	39.0
12	36.61309040294617	39.0	35.0	39.0	32.0	39.0
13	36.6553958795094	39.0	35.0	39.0	32.0	39.0
14	37.72423359215988	40.0	37.0	41.0	32.0	41.0
15	37.72275902010728	40.0	37.0	41.0	32.0	41.0
16	37.68410213609543	39.0	36.0	41.0	32.0	41.0
17	37.68101651424465	39.0	37.0	41.0	32.0	41.0
18	37.64073020843039	39.0	36.0	41.0	32.0	41.0
19	37.6812032962875	40.0	37.0	41.0	32.0	41.0
20	37.63609521422413	39.0	37.0	41.0	32.0	41.0
21	37.59047140375672	39.0	36.0	41.0	32.0	41.0
22	37.4813395115759	39.0	36.0	41.0	32.0	41.0
23	37.38937538247669	39.0	36.0	41.0	32.0	41.0
24	37.32769269061064	39.0	36.0	41.0	31.0	41.0
25	37.26011389767756	39.0	36.0	41.0	31.0	41.0
26	37.05907824155424	39.0	36.0	41.0	31.0	41.0
27	36.90291926772691	39.0	36.0	40.0	31.0	41.0
28	36.81353728007727	39.0	35.0	40.0	30.0	41.0
29	36.68668952668905	39.0	35.0	40.0	30.0	41.0
30	36.527816745381955	38.0	35.0	40.0	30.0	41.0
31	36.35586309707374	38.0	35.0	40.0	30.0	41.0
32	36.20876939504052	38.0	35.0	40.0	30.0	41.0
33	36.35497511687001	38.0	35.0	40.0	30.0	41.0
34	36.372840141254464	38.0	35.0	40.0	30.0	41.0
35	36.349867699698216	38.0	35.0	40.0	30.0	41.0
36	36.28818981954755	38.0	35.0	40.0	30.0	41.0
37	36.19305345772055	38.0	35.0	40.0	30.0	41.0
38	36.11404815302305	38.0	35.0	40.0	29.0	41.0
39	36.10924606106417	38.0	35.0	40.0	29.0	41.0
40	36.03117947829632	38.0	35.0	40.0	29.0	41.0
41	35.94099699617731	38.0	35.0	40.0	28.0	41.0
42	35.85751285930938	38.0	35.0	40.0	28.0	41.0
43	35.53935173945698	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	1.0
15	5.0
16	18.0
17	45.0
18	117.0
19	272.0
20	588.0
21	1210.0
22	2267.0
23	3993.0
24	6511.0
25	10647.0
26	16242.0
27	23686.0
28	33659.0
29	46091.0
30	60306.0
31	75959.0
32	93461.0
33	115555.0
34	143282.0
35	177185.0
36	225355.0
37	301799.0
38	428613.0
39	519216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15092732691276	21.405834511110033	12.88013098364148	24.563107178335734
2	18.257047960117003	22.81606080608481	35.02688217902468	23.900009054773506
3	19.487884756791846	25.532142914945933	31.01115574341659	23.968816584845634
4	13.857302893546922	16.74201375538201	36.20548999228813	33.19519335878293
5	13.33759388859654	38.348278083904944	34.5694630169368	13.744665010561716
6	32.14383354614238	37.165777155462585	16.22786009456333	14.4625292038317
7	26.400045142638927	32.72237670744814	22.839419497158246	18.038158652754685
8	26.457392041510232	34.18356344268613	20.716446924373393	18.642597591430246
9	26.229491703509094	14.097538720092457	21.5888546673858	38.08411490901265
10	16.076116088320347	27.45687281367682	34.53844932410709	21.928561773895744
11	33.688437929090185	22.74414753244299	23.310792633876137	20.256621904590684
12	20.34108938111279	26.70257081204696	31.406241319774796	21.55009848706545
13	29.468869732429255	21.370490274429628	26.66840763278038	22.492232360360738
14	21.424993886934313	22.03092008309395	27.394889170884575	29.14919685908717
15	23.742403504328575	29.179685637510733	24.40213342711804	22.67577743104265
16	23.04028674324293	27.81031518048088	25.9811634465355	23.168234629740688
17	21.53238262585807	27.57957155611313	27.36938707931938	23.51865873870942
18	21.87055873201676	26.843554072963975	29.10177959106543	22.18410760395383
19	22.109438529679753	26.394139855569804	29.761465770987716	21.734955843762727
20	23.346311842025262	26.03649817351658	29.0596989528395	21.557491031618657
21	23.17877666073076	26.373449479394267	28.535965604108682	21.911808255766292
22	22.996237675993957	26.191697866266683	28.828999071338927	21.98306538640043
23	22.35776678665335	26.580265755415255	28.880790626078536	22.181176831852856
24	21.974754241636475	26.773827942681095	28.533166060609243	22.718251755073187
25	22.578930723108964	26.834455556590804	28.76290359903188	21.82371012126835
26	22.628491391622454	26.724048559831715	28.46777047417706	22.17968957436878
27	22.41633848580566	26.955360841472785	28.357275991683608	22.27102468103795
28	22.180170745907745	26.989873963676796	28.597424332494782	22.232530957920673
29	21.796458315016007	26.900463543163493	29.00541405467071	22.297664087149787
30	21.82541609308832	27.300185863442643	29.063810782354306	21.810587261114733
31	22.28764697056586	26.890008997907778	28.771171000928664	22.0511730305977
32	22.233230843795535	27.05417597842952	28.42726457916956	22.28532859860539
33	22.49494441812582	26.963671986236744	28.322019240737557	22.219364354899877
34	22.23244347218632	27.138424740615736	28.59681193235428	22.032319854843667
35	22.050648116191553	27.260773540114613	28.785649889964816	21.902928453729015
36	21.967580411419164	26.64548637037873	29.06018012437847	22.32675309382364
37	21.847506241013576	26.346766330415246	29.135986513199192	22.669740915371985
38	21.822572806721706	26.350003302586472	29.29805383609635	22.52937005459547
39	22.021252909447455	26.016332711747193	29.607972050057583	22.354442328747766
40	21.629929219666618	26.037722973797585	29.73495759347741	22.597390213058382
41	21.32687863585244	25.73261647522601	29.990503423535497	22.95000146538605
42	20.670648142437273	26.38587245367302	29.86535508053718	23.078124323352522
43	20.783329768289658	25.902907457152768	30.006513312922912	23.30724946163466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	356.0
1	429.5
2	503.0
3	1231.0
4	1959.0
5	1959.0
6	2931.0
7	3903.0
8	3928.0
9	3953.0
10	5605.0
11	7257.0
12	7257.0
13	11905.5
14	16554.0
15	24496.0
16	32438.0
17	32175.5
18	31913.0
19	31913.0
20	35381.5
21	38850.0
22	35259.5
23	31669.0
24	37436.5
25	43204.0
26	43204.0
27	49745.5
28	56287.0
29	62201.5
30	68116.0
31	75547.5
32	82979.0
33	82979.0
34	91159.5
35	99340.0
36	108360.0
37	117380.0
38	124915.0
39	132450.0
40	132450.0
41	138160.5
42	143871.0
43	150367.0
44	156863.0
45	165892.5
46	174922.0
47	174922.0
48	176583.5
49	178245.0
50	167961.5
51	157678.0
52	150323.0
53	142968.0
54	142968.0
55	133886.5
56	124805.0
57	116558.5
58	108312.0
59	100169.5
60	92027.0
61	92027.0
62	82140.0
63	72253.0
64	62506.0
65	52759.0
66	45327.0
67	37895.0
68	37895.0
69	32681.5
70	27468.0
71	23713.0
72	19958.0
73	15810.5
74	11663.0
75	11663.0
76	9159.0
77	6655.0
78	5148.5
79	3642.0
80	2988.5
81	2335.0
82	2335.0
83	1717.5
84	1100.0
85	899.0
86	698.0
87	581.0
88	464.0
89	464.0
90	383.0
91	302.0
92	180.0
93	58.0
94	44.0
95	30.0
96	30.0
97	17.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2286087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.32273542738744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59614179426283	39.5539692643248
2	13.301171876074507	13.919073938921061
3	4.706467203994668	7.387657149368669
4	2.0971032617826064	4.389047165206502
5	1.1473282782970282	3.0015676976847683
6	0.6558958545153535	2.059095915823623
7	0.4566922980020453	1.672677319606062
8	0.3298266411875643	1.3805945667008621
9	0.24509844968581296	1.1541799202916263
>10	1.291890309262561	12.40318230314446
>50	0.10809372364772385	3.9729055904492014
>100	0.060852006706142096	5.554269065334559
>500	0.0020126649255775807	0.7129799483110315
>1k	0.001090193501354481	0.8994631029864857
>5k	8.386103856572932E-5	0.2318534092482848
>10k+	2.51583115697188E-4	1.7074836425979851
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13643	0.5967839369192861	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13619	0.5957341081069968	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11652	0.5096918883664533	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5284	0.23113731017236003	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3081	0.13477177377763838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.374286717872067E-5	0.0
9	0.0	0.0	0.0	8.748573435744135E-5	0.0
10	0.0	0.0	0.0	8.748573435744135E-5	0.0
11	0.0	0.0	0.0	1.749714687148827E-4	0.0
12	0.0	0.0	0.0	2.1871433589360336E-4	0.0
13	0.0	0.0	0.0	2.1871433589360336E-4	0.0
14	0.0	0.0	0.0	2.62457203072324E-4	0.0
15	0.0	0.0	0.0	3.499429374297654E-4	0.0
16	0.0	0.0	0.0	6.998858748595308E-4	0.0
17	0.0	0.0	0.0	0.0012248002810041788	0.0
18	0.0	0.0	0.0	0.001574743218433944	0.0
19	0.0	0.0	0.0	0.0016622289527913855	0.0
20	0.0	0.0	0.0	0.0018809432886849888	0.0
21	0.0	0.0	0.0	0.002405857694829637	0.0
22	0.0	0.0	0.0	0.003455686507118933	0.0
23	0.0	0.0	0.0	0.0054678583973400835	0.0
24	0.0	0.0	0.0	0.010760745325965285	0.0
25	0.0	0.0	0.0	0.015222517778194793	0.0
26	0.0	0.0	0.0	0.02357740540933044	0.0
27	0.0	0.0	0.0	0.07878090378887592	0.0
28	0.0	0.0	0.0	0.13958348916729765	0.0
29	0.0	0.0	0.0	0.21346519183215687	0.0
30	0.0	0.0	0.0	0.32479078880200096	0.0
31	0.0	0.0	0.0	0.5363750373454729	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	70	6.5992936E-9	26.42857	14
GGTATCA	3975	0.0	21.594969	1
TATACCG	165	3.6379788E-12	17.939394	5
GTATTGG	260	0.0	17.076923	1
CGGGATA	145	2.9849616E-9	16.586206	24
ACGTTTA	145	5.3525582E-8	15.310345	26
GTACTAG	200	6.184564E-11	14.799999	1
CCGTCGT	140	6.0018283E-7	14.535714	9
TACGTGC	140	6.0018283E-7	14.535714	8
ATACCGA	245	0.0	14.34694	6
ATAGACG	105	1.6567612E-4	14.095239	3
ACTACGT	160	1.785611E-7	13.875001	6
TGCGACG	135	6.5733E-6	13.703704	22
CAATGCG	135	6.5733E-6	13.703704	19
GCGCGAT	150	1.3070003E-6	13.566666	13
GTATCAA	6330	0.0	13.5608225	2
TACCGAC	210	2.044544E-9	13.214287	7
TACTCCG	170	3.7356585E-7	13.058824	5
GCGCTAG	85	0.009408451	13.058824	11
ATACCGC	185	7.392555E-8	13.000001	27
>>END_MODULE