FastQCFastQC Report
Wed 25 May 2016
SRR2088377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078903
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318821.0354986824852876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT309311.004611057899518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281780.915196094193289No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG131000.4254762166914645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104870.34060832705674715No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT70110.22771097368121046No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69980.2272887453745701No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT66690.2166031213065173No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62600.20331916919760057No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA57170.1856830176202368No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT54980.1785700946083719No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT49490.16073906842794333No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG49310.16015444461874895No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41630.13521049542645547No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.1315078779682244No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39780.12920186183195767No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT37530.12189406421702796No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37300.12114704490527957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.026.8863331
ATTGGAC8750.020.0857143
GTATTGG9400.018.6968081
TGCGACG1054.803733E-717.61904722
CGAACGA2050.017.14634116
TTGGACC15450.016.1650494
GGACCCT14850.016.0707076
GTATCAA126550.015.744372
TGGACCC16300.014.9815955
TCGACGG750.004106487614.819
TATTGGA13200.014.5757592
ATAACGC2059.276846E-1114.4390243
CGCGATA908.2795776E-414.38888814
CGTCGTA1956.7484507E-1014.2307710
CAATGCG1304.449512E-614.23076919
GTACTAG2750.014.1272721
CGTATAC1051.6570564E-414.0952383
CCGATAA2500.014.0599999
CTAGTAC3950.014.0506323
TCTATAC3200.013.8753
Produced by FastQC (version 0.11.5)