##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088377_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3078903
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409691698634223	31.0	31.0	34.0	30.0	34.0
2	31.60900554515683	31.0	31.0	34.0	30.0	34.0
3	31.696078440925227	31.0	31.0	34.0	30.0	34.0
4	35.483775227735336	37.0	35.0	37.0	33.0	37.0
5	35.302605830713084	37.0	35.0	37.0	33.0	37.0
6	35.328924945021	37.0	35.0	37.0	32.0	37.0
7	35.215953864087304	37.0	35.0	37.0	32.0	37.0
8	35.24340000318295	37.0	35.0	37.0	32.0	37.0
9	36.75453854830763	39.0	37.0	39.0	32.0	39.0
10	36.61514766785443	39.0	35.0	39.0	32.0	39.0
11	36.721650211130395	39.0	35.0	39.0	32.0	39.0
12	36.59528020207197	39.0	35.0	39.0	32.0	39.0
13	36.62644357422108	39.0	35.0	39.0	32.0	39.0
14	37.64939590497005	39.0	36.0	41.0	32.0	41.0
15	37.63713244619918	39.0	36.0	41.0	32.0	41.0
16	37.59238404067943	39.0	36.0	41.0	32.0	41.0
17	37.6489411975629	39.0	36.0	41.0	32.0	41.0
18	37.62750369206175	39.0	36.0	41.0	32.0	41.0
19	37.681045489253805	40.0	37.0	41.0	32.0	41.0
20	37.64327489368778	39.0	37.0	41.0	32.0	41.0
21	37.591815331629476	39.0	36.0	41.0	32.0	41.0
22	37.482246761265294	39.0	36.0	41.0	32.0	41.0
23	37.381213698515346	39.0	36.0	41.0	32.0	41.0
24	37.30806459313593	39.0	36.0	41.0	31.0	41.0
25	37.245420528025726	39.0	36.0	41.0	31.0	41.0
26	37.03134265678393	39.0	36.0	41.0	31.0	41.0
27	36.86753658689475	39.0	35.0	40.0	31.0	41.0
28	36.75671887032492	39.0	35.0	40.0	30.0	41.0
29	36.612126461924916	39.0	35.0	40.0	30.0	41.0
30	36.43650904234398	38.0	35.0	40.0	30.0	41.0
31	36.21459461373093	38.0	35.0	40.0	30.0	41.0
32	36.068789435717854	38.0	35.0	40.0	30.0	41.0
33	36.14529980320913	38.0	35.0	40.0	30.0	41.0
34	36.118713061113	38.0	35.0	40.0	30.0	41.0
35	36.048592631856216	38.0	35.0	40.0	29.0	41.0
36	35.96895030470268	38.0	35.0	40.0	29.0	41.0
37	35.846948734662966	38.0	35.0	40.0	28.0	41.0
38	35.73532456202745	38.0	35.0	40.0	28.0	41.0
39	35.67388871945625	38.0	35.0	40.0	27.0	41.0
40	35.53580869549966	38.0	34.0	40.0	26.0	41.0
41	35.404956570570754	38.0	34.0	40.0	26.0	41.0
42	35.25562318786918	38.0	34.0	40.0	25.0	41.0
43	34.93008126595739	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	0.0
14	4.0
15	9.0
16	26.0
17	85.0
18	208.0
19	433.0
20	934.0
21	1860.0
22	3399.0
23	6019.0
24	10072.0
25	15864.0
26	24051.0
27	35176.0
28	49642.0
29	67398.0
30	86631.0
31	107032.0
32	130424.0
33	160398.0
34	196637.0
35	241202.0
36	305834.0
37	415169.0
38	555917.0
39	664476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64155220219669	21.769474387468524	13.28694018616371	23.302033224171076
2	18.098036865727828	22.8617790167472	35.640616154519975	23.399567963005005
3	19.69500175874329	25.923746217402755	31.683817255691395	22.697434768162555
4	13.184631019554693	16.084235196756765	35.85143799593556	34.87969578775297
5	12.311203048618289	39.47055168675337	34.41436771473476	13.803877549893581
6	31.595604018704066	36.71327092798961	15.867794470952804	15.82333058235352
7	25.428927121120736	34.29646857988056	21.967174672277757	18.307429626720946
8	27.51028531915426	33.35476953967046	20.43861076493803	18.69633437623725
9	25.951191057334384	13.684127106310267	22.64095361237428	37.72372822398108
10	16.374955625428928	27.289362477479806	33.77638074340114	22.55930115369013
11	32.654715007260705	22.190825758395118	24.253930701941567	20.900528532402614
12	20.450887864931115	27.12008140561752	31.49949836029261	20.929532369158753
13	30.371174408547457	20.92972724376182	27.8129905359149	20.886107811775815
14	21.172378603678	22.324802048002162	28.070452365664007	28.43236698265584
15	23.450397755304405	28.85004821522471	25.85362383939994	21.845930190070945
16	22.46413738919349	28.03157488235258	26.621754566480334	22.882533161973598
17	20.487167020201678	27.10569316409124	27.63887007807651	24.768269737630575
18	21.311226758361663	26.206606703751305	30.89405544767081	21.588111090216223
19	20.957854144804173	25.415415815308247	31.856346237604754	21.770383802282826
20	24.00803143197431	24.47264496478129	30.807368728407486	20.711954874836913
21	23.65998539090059	25.35354312883517	29.90987374399258	21.07659773627165
22	22.60051713223833	24.923422400770665	30.73562239537913	21.74043807161187
23	21.742029547536898	26.020306583221362	31.282408052478434	20.95525581676331
24	20.636376007948286	25.827088414282617	30.39462431911626	23.14191125865284
25	21.667912240171255	26.237137058231454	31.01247424813318	21.082476453464107
26	22.527601551591587	25.94044047506531	29.94917345561065	21.582784517732453
27	21.680351735666893	26.73390490054412	29.81042273822852	21.775320625560468
28	21.340782739826487	26.39378375999504	30.008220460339285	22.25721303983919
29	20.639591438898854	26.329085391777525	30.78729664429181	22.244026525031803
30	20.294468516871106	26.735918604775794	31.791940181291846	21.177672697061258
31	21.319898678198047	26.31024751348126	30.896004193701458	21.473849614619233
32	21.9802312706831	26.27731370556331	29.703923767653613	22.03853125609998
33	22.41408709530635	26.17178910800373	29.571376558469037	21.842747238220888
34	21.545401073044523	27.88454199434019	29.472770009318257	21.09728692329703
35	20.687498112152284	28.34616744990018	29.890191409083037	21.076143028864504
36	21.142952538615216	26.28234796614249	30.954791365626004	21.619908129616295
37	21.266535516058806	26.185787600323884	30.50547548915961	22.0422013944577
38	21.212685167411898	25.87856778859224	31.029168505795734	21.879578538200132
39	21.936579359596582	25.56004524988283	30.978371192596843	21.525004197923742
40	20.757847843858674	25.888473914248028	30.64867584331172	22.705002398581573
41	21.082151662459	25.6609902942704	30.688917448844606	22.567940594426002
42	19.66606288018817	26.712566131508524	30.998703109516608	22.622667878786697
43	19.93573685172933	26.272701673290776	30.717466578193598	23.074094896786292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	382.0
2	525.0
3	1457.5
4	2390.0
5	2390.0
6	3969.5
7	5549.0
8	5833.5
9	6118.0
10	9020.5
11	11923.0
12	11923.0
13	21028.5
14	30134.0
15	48265.0
16	66396.0
17	65681.0
18	64966.0
19	64966.0
20	69928.5
21	74891.0
22	62724.0
23	50557.0
24	56790.0
25	63023.0
26	63023.0
27	68644.5
28	74266.0
29	79319.0
30	84372.0
31	90737.5
32	97103.0
33	97103.0
34	106294.0
35	115485.0
36	126837.5
37	138190.0
38	144995.5
39	151801.0
40	151801.0
41	160128.0
42	168455.0
43	184128.5
44	199802.0
45	234385.5
46	268969.0
47	268969.0
48	273689.0
49	278409.0
50	256362.5
51	234316.0
52	223032.0
53	211748.0
54	211748.0
55	190690.5
56	169633.0
57	149555.0
58	129477.0
59	117020.0
60	104563.0
61	104563.0
62	93402.5
63	82242.0
64	72793.5
65	63345.0
66	54577.5
67	45810.0
68	45810.0
69	38656.5
70	31503.0
71	26896.0
72	22289.0
73	17579.5
74	12870.0
75	12870.0
76	9891.0
77	6912.0
78	5625.0
79	4338.0
80	3621.5
81	2905.0
82	2905.0
83	2175.0
84	1445.0
85	1163.5
86	882.0
87	751.5
88	621.0
89	621.0
90	476.0
91	331.0
92	198.0
93	65.0
94	50.5
95	36.0
96	36.0
97	21.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3078903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.59787906411692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80950563423421	32.179379004236
2	13.741651195945137	11.982136939641874
3	5.080153578498489	6.644517640275538
4	2.4254564628017774	4.229790301620507
5	1.314340605824548	2.8651231390898397
6	0.7675975987028868	2.0079376366893005
7	0.5183884555848361	1.582044603637542
8	0.3573451144875154	1.2463591268463767
9	0.2583485319242195	1.0137103246101844
>10	1.4692091263724676	11.975931441137137
>50	0.14018220927841937	4.272988858220446
>100	0.10370790225562872	8.635305403335066
>500	0.008288930338559507	2.369772341106252
>1k	0.005003228222342215	4.022580216464476
>5k	4.4805028856795953E-4	1.2421371928418625
>10k+	3.7337524047329965E-4	3.7302858302475537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31882	1.0354986824852876	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30931	1.004611057899518	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28178	0.915196094193289	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13100	0.4254762166914645	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10487	0.34060832705674715	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7011	0.22771097368121046	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6998	0.2272887453745701	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6669	0.2166031213065173	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6260	0.20331916919760057	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5717	0.1856830176202368	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5498	0.1785700946083719	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4949	0.16073906842794333	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4931	0.16015444461874895	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4163	0.13521049542645547	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4049	0.1315078779682244	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3978	0.12920186183195767	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3753	0.12189406421702796	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3730	0.12114704490527957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.247910051079881E-5	0.0
2	0.0	0.0	0.0	3.247910051079881E-5	0.0
3	3.247910051079881E-5	0.0	0.0	3.247910051079881E-5	0.0
4	3.247910051079881E-5	0.0	0.0	3.247910051079881E-5	0.0
5	3.247910051079881E-5	0.0	0.0	3.247910051079881E-5	0.0
6	3.247910051079881E-5	0.0	0.0	3.247910051079881E-5	0.0
7	3.247910051079881E-5	0.0	0.0	3.247910051079881E-5	0.0
8	3.247910051079881E-5	0.0	0.0	6.495820102159763E-5	0.0
9	3.247910051079881E-5	0.0	0.0	6.495820102159763E-5	0.0
10	3.247910051079881E-5	0.0	0.0	1.2991640204319525E-4	0.0
11	3.247910051079881E-5	0.0	0.0	3.5727010561878696E-4	0.0
12	3.247910051079881E-5	0.0	0.0	6.495820102159763E-4	0.0
13	3.247910051079881E-5	0.0	0.0	6.82061110726775E-4	0.0
14	3.247910051079881E-5	0.0	0.0	8.444566132807692E-4	0.0
15	3.247910051079881E-5	0.0	0.0	0.0012666849199211537	0.0
16	3.247910051079881E-5	0.0	0.0	0.0018513087291155323	0.0
17	3.247910051079881E-5	0.0	0.0	0.002630807141374704	0.0
18	3.247910051079881E-5	0.0	0.0	0.002955598146482692	0.0
19	3.247910051079881E-5	0.0	0.0	0.0032479100510798813	0.0
20	3.247910051079881E-5	0.0	0.0	0.0038974920612958575	0.0
21	3.247910051079881E-5	0.0	0.0	0.005294093383260206	0.0
22	3.247910051079881E-5	0.0	0.0	0.00850952433382929	0.0
23	3.247910051079881E-5	0.0	0.0	0.013803617717089496	0.0
24	3.247910051079881E-5	0.0	0.0	0.022053309246832395	0.0
25	3.247910051079881E-5	0.0	0.0	0.027834589137754582	0.0
26	3.247910051079881E-5	0.0	0.0	0.036019322466475885	0.0
27	3.247910051079881E-5	0.0	0.0	0.10185445920186507	0.0
28	3.247910051079881E-5	0.0	0.0	0.17269137741591728	0.0
29	3.247910051079881E-5	0.0	0.0	0.2527198810745256	0.0
30	3.247910051079881E-5	0.0	0.0	0.3910158910495069	0.0
31	3.247910051079881E-5	0.0	0.0	0.6265868070543307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7390	0.0	26.886333	1
ATTGGAC	875	0.0	20.085714	3
GTATTGG	940	0.0	18.696808	1
TGCGACG	105	4.803733E-7	17.619047	22
CGAACGA	205	0.0	17.146341	16
TTGGACC	1545	0.0	16.165049	4
GGACCCT	1485	0.0	16.070707	6
GTATCAA	12655	0.0	15.74437	2
TGGACCC	1630	0.0	14.981595	5
TCGACGG	75	0.0041064876	14.8	19
TATTGGA	1320	0.0	14.575759	2
ATAACGC	205	9.276846E-11	14.439024	3
CGCGATA	90	8.2795776E-4	14.388888	14
CGTCGTA	195	6.7484507E-10	14.23077	10
CAATGCG	130	4.449512E-6	14.230769	19
GTACTAG	275	0.0	14.127272	1
CGTATAC	105	1.6570564E-4	14.095238	3
CCGATAA	250	0.0	14.059999	9
CTAGTAC	395	0.0	14.050632	3
TCTATAC	320	0.0	13.875	3
>>END_MODULE