FastQCFastQC Report
Wed 25 May 2016
SRR2088376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413864
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT367621.0768443031122505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364391.0673828834423398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320660.939287563886552No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG169790.4973543175709401No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123970.36313690293462186No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95390.2794194496324399No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT88780.26005722547822646No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87020.2549017769893587No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT84890.24866251262499034No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA73330.21480058959583628No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT71180.20850274058954896No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG62640.1834870984901566No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59790.175138787016706No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55150.1615471500915092No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51280.15021102188019206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.13506689194414306No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT44560.13052658219542432No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44540.13046799755350535No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37150.1088209723644527No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35690.10454429350436924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89600.027.6880571
GTATTGG10150.018.7733971
ATTGGAC10100.017.9504953
GGACCCT17200.016.4563966
TTGGACC18150.016.4104674
GTATCAA152000.016.3335532
CCCGTAT1455.3552867E-815.3103451
GTATACT3550.015.1126764
TGGACCC18000.015.1083345
TCTAACG1406.0043385E-714.5357142
CGTCTGT7800.014.46794834
CGTTTAG2750.014.12727226
TATTGGA16000.014.1062512
TATACTG4600.014.0760875
GACCCTC18300.014.0519137
GCGGTTA950.001245953413.63157930
AATGCGT2900.013.39655135
ATTCGTC1805.1684765E-813.36111117
CGAATTA2800.013.21428515
TGCGTCT8500.012.84117732
Produced by FastQC (version 0.11.5)