##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3413864
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3542337949022	31.0	31.0	34.0	30.0	34.0
2	31.55888811036409	31.0	31.0	34.0	30.0	34.0
3	31.64117668424987	31.0	31.0	34.0	30.0	34.0
4	35.43936870361561	37.0	35.0	37.0	33.0	37.0
5	35.27016278328603	37.0	35.0	37.0	33.0	37.0
6	35.29799224573679	37.0	35.0	37.0	32.0	37.0
7	35.18373520444868	37.0	35.0	37.0	32.0	37.0
8	35.215776023883784	37.0	35.0	37.0	32.0	37.0
9	36.72163302345963	39.0	35.0	39.0	32.0	39.0
10	36.57842638136727	39.0	35.0	39.0	32.0	39.0
11	36.68482077786344	39.0	35.0	39.0	32.0	39.0
12	36.54795445864276	39.0	35.0	39.0	32.0	39.0
13	36.58260580972177	39.0	35.0	39.0	32.0	39.0
14	37.598135719524855	39.0	36.0	41.0	32.0	41.0
15	37.58863680568412	39.0	36.0	41.0	32.0	41.0
16	37.544262454509024	39.0	36.0	41.0	32.0	41.0
17	37.59928515019931	39.0	36.0	41.0	32.0	41.0
18	37.570480253460595	39.0	36.0	41.0	32.0	41.0
19	37.62919905420954	39.0	37.0	41.0	32.0	41.0
20	37.58809929159451	39.0	36.0	41.0	32.0	41.0
21	37.53671851016912	39.0	36.0	41.0	32.0	41.0
22	37.42539919575004	39.0	36.0	41.0	32.0	41.0
23	37.320196703793705	39.0	36.0	41.0	31.0	41.0
24	37.24736251942081	39.0	36.0	41.0	31.0	41.0
25	37.19384603487426	39.0	36.0	41.0	31.0	41.0
26	36.9684173124647	39.0	36.0	40.0	31.0	41.0
27	36.811891744955275	39.0	35.0	40.0	30.0	41.0
28	36.692514698886654	39.0	35.0	40.0	30.0	41.0
29	36.54651327645155	38.0	35.0	40.0	30.0	41.0
30	36.36711772935301	38.0	35.0	40.0	30.0	41.0
31	36.1387290179105	38.0	35.0	40.0	30.0	41.0
32	36.0080811655063	38.0	35.0	40.0	30.0	41.0
33	36.087415608823314	38.0	35.0	40.0	30.0	41.0
34	36.0667589570059	38.0	35.0	40.0	30.0	41.0
35	36.00010662404829	38.0	35.0	40.0	29.0	41.0
36	35.919559478643556	38.0	35.0	40.0	29.0	41.0
37	35.79318449709772	38.0	35.0	40.0	28.0	41.0
38	35.68263615656628	38.0	35.0	40.0	27.0	41.0
39	35.62778540680004	38.0	35.0	40.0	27.0	41.0
40	35.47887496397045	38.0	34.0	40.0	26.0	41.0
41	35.35063699081159	38.0	34.0	40.0	26.0	41.0
42	35.19139895438131	38.0	34.0	40.0	25.0	41.0
43	34.85682968038563	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	16.0
16	30.0
17	88.0
18	191.0
19	494.0
20	1074.0
21	2137.0
22	4049.0
23	6914.0
24	11366.0
25	18186.0
26	27428.0
27	40457.0
28	56482.0
29	75826.0
30	97966.0
31	119860.0
32	146821.0
33	179221.0
34	219851.0
35	269036.0
36	342807.0
37	466826.0
38	625432.0
39	701300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35636334663595	22.203960087455155	13.516297075689012	22.92337949021988
2	18.235436443865368	23.03433880201437	35.62942167584883	23.100803078271426
3	19.849501913374404	26.058419433228742	31.863425139372865	22.228653514023993
4	13.17047193444144	16.185149730627817	35.87565292583419	34.768725409096554
5	12.075437100013357	39.7867636203434	34.51408726299583	13.623712016647413
6	31.504975007791757	36.870654484185664	15.743070022707407	15.881300485315172
7	25.34983818921902	34.34486552481294	21.903830966904366	18.401465319063675
8	27.631417068752594	33.33852783825015	20.38903717312699	18.64101791987027
9	25.822821295751673	13.589000616310434	22.756003168257436	37.832174919680455
10	16.330176011698182	27.36553067140343	33.86201090611694	22.44228241078145
11	32.57021955180405	22.031135393794248	24.408148655013793	20.990496399387908
12	20.430749438173283	27.173607384476945	31.439037993311974	20.956605184037794
13	30.63566679867739	20.724785755964504	28.066056527149296	20.573490918208808
14	21.172138081657618	22.21878200186065	28.062922248806633	28.546157667675104
15	23.30511701696377	28.9554299761209	25.949217660691815	21.790235346223515
16	22.391606695521553	28.10199820496657	26.65703730435659	22.849357795155285
17	20.374859689782603	27.00722700142712	27.677171674091294	24.94074163469898
18	21.288457888187693	26.0561346321939	31.241226949872637	21.414180529745767
19	20.819839337478	25.064355229147967	32.14184865009268	21.97395678328135
20	24.095013743956994	24.25530132424725	31.040896766830784	20.608788164964977
21	23.78846960511608	25.050529253655096	30.14727593132005	21.01372520990877
22	22.51782730653594	24.662845385756434	31.03934427381993	21.7799830338877
23	21.660528949014957	25.843794597558663	31.800798157161502	20.694878296264875
24	20.590041079550915	25.488039359505827	30.60113115226617	23.32078840867709
25	21.66820353710634	26.123653432005494	31.22505758870301	20.98308544218516
26	22.577935149144782	25.655767189319782	30.191741674536537	21.5745559869989
27	21.575053956455207	26.60167481774318	29.98640250461061	21.836868721191003
28	21.352432317163192	26.154058861161428	30.20008998600999	22.293418835665392
29	20.454183294940865	26.176057394201997	31.050328894179735	22.319430416677406
30	20.152120881206752	26.552112210679745	32.148878807122955	21.146888100990548
31	21.302899002420716	26.03120686705739	31.27069502475787	21.395199105764025
32	22.100968286961635	25.98530580011389	29.804526483773223	22.10919942915125
33	22.53393808306365	25.969224315907134	29.648251951454423	21.848585649574794
34	21.585950699852134	27.977125040716327	29.556859910060858	20.880064349370684
35	20.52026677102544	28.6971595822212	29.971170497711686	20.81140314904167
36	21.109247468557623	26.22110898383767	31.198899546086196	21.47074400151851
37	21.412891667623548	26.147878181438976	30.60716537038382	21.832064780553647
38	21.327006582570366	25.84739755303668	31.16052660562928	21.665069258763676
39	22.17106481101766	25.5432846768354	30.947512847611975	21.33813766453497
40	20.769866637921137	26.06899396109511	30.57658418730213	22.58455521368162
41	21.31441088455779	25.687900865412335	30.595360565037154	22.402327684992724
42	19.722695455940833	26.97482969444594	30.892706915096795	22.40976793451643
43	20.03477584344309	26.606039373566144	30.563549104475165	22.795635678515605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	372.5
2	522.0
3	1457.5
4	2393.0
5	2393.0
6	4137.5
7	5882.0
8	6281.5
9	6681.0
10	9891.5
11	13102.0
12	13102.0
13	23390.0
14	33678.0
15	54730.0
16	75782.0
17	73942.0
18	72102.0
19	72102.0
20	78504.5
21	84907.0
22	70904.0
23	56901.0
24	63437.0
25	69973.0
26	69973.0
27	75966.5
28	81960.0
29	87309.0
30	92658.0
31	99887.0
32	107116.0
33	107116.0
34	117413.5
35	127711.0
36	140573.5
37	153436.0
38	160044.0
39	166652.0
40	166652.0
41	175552.0
42	184452.0
43	202130.0
44	219808.0
45	261355.0
46	302902.0
47	302902.0
48	309291.0
49	315680.0
50	290413.0
51	265146.0
52	253163.0
53	241180.0
54	241180.0
55	214066.5
56	186953.0
57	162850.5
58	138748.0
59	125034.0
60	111320.0
61	111320.0
62	100024.0
63	88728.0
64	78113.0
65	67498.0
66	58073.0
67	48648.0
68	48648.0
69	41367.5
70	34087.0
71	29306.5
72	24526.0
73	19133.5
74	13741.0
75	13741.0
76	10477.5
77	7214.0
78	6007.5
79	4801.0
80	3898.5
81	2996.0
82	2996.0
83	2253.5
84	1511.0
85	1248.5
86	986.0
87	848.0
88	710.0
89	710.0
90	561.5
91	413.0
92	254.5
93	96.0
94	62.0
95	28.0
96	28.0
97	18.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3413864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.97464700191364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34142478761063	30.05138991289745
2	13.723105482082254	11.245988057966926
3	5.188387920228854	6.377770906211105
4	2.4262570559396495	3.976601056121175
5	1.3300080236409066	2.724830463919948
6	0.8315118174193115	2.0442541918005577
7	0.5434370542092783	1.5586999024789492
8	0.37809840662531935	1.2393958994766774
9	0.28349469007467987	1.0454485367454225
>10	1.6622334517190374	12.85718021610103
>50	0.16076083536727304	4.600506548902265
>100	0.11469860896009931	9.182652563020671
>500	0.01000629852246735	2.8157355314480865
>1k	0.005503464187142124	4.200115893553889
>5k	7.147356087197563E-4	2.1362701343305774
>10k+	3.5736780435987816E-4	3.9431601850253197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36762	1.0768443031122505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36439	1.0673828834423398	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32066	0.939287563886552	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16979	0.4973543175709401	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12397	0.36313690293462186	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9539	0.2794194496324399	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8878	0.26005722547822646	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8702	0.2549017769893587	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8489	0.24866251262499034	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7333	0.21480058959583628	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7118	0.20850274058954896	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6264	0.1834870984901566	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5979	0.175138787016706	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5515	0.1615471500915092	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5128	0.15021102188019206	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4611	0.13506689194414306	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4456	0.13052658219542432	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4454	0.13046799755350535	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3715	0.1088209723644527	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3569	0.10454429350436924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.929232095947583E-5	0.0
3	0.0	0.0	0.0	5.858464191895166E-5	0.0
4	0.0	0.0	0.0	5.858464191895166E-5	0.0
5	2.929232095947583E-5	0.0	0.0	5.858464191895166E-5	0.0
6	2.929232095947583E-5	0.0	0.0	5.858464191895166E-5	0.0
7	2.929232095947583E-5	0.0	0.0	5.858464191895166E-5	0.0
8	2.929232095947583E-5	0.0	0.0	5.858464191895166E-5	0.0
9	2.929232095947583E-5	0.0	0.0	5.858464191895166E-5	0.0
10	2.929232095947583E-5	0.0	0.0	8.78769628784275E-5	0.0
11	2.929232095947583E-5	0.0	0.0	2.3433856767580665E-4	0.0
12	5.858464191895166E-5	0.0	0.0	4.100924934326616E-4	0.0
13	5.858464191895166E-5	0.0	0.0	4.3938481439213746E-4	0.0
14	8.78769628784275E-5	0.0	0.0	6.151387401489924E-4	0.0
15	8.78769628784275E-5	0.0	0.0	9.080619497437508E-4	0.0
16	8.78769628784275E-5	0.0	0.0	0.0012888621222169367	0.0
17	8.78769628784275E-5	0.0	0.0	0.0019625855042848805	0.0
18	8.78769628784275E-5	0.0	0.0	0.002401970318677018	0.0
19	8.78769628784275E-5	0.0	0.0	0.00281206281210968	0.0
20	8.78769628784275E-5	0.0	0.0	0.0033979092312991965	0.0
21	8.78769628784275E-5	0.0	0.0	0.004979694563110891	0.0
22	8.78769628784275E-5	0.0	0.0	0.008758403966883273	0.0
23	8.78769628784275E-5	0.0	0.0	0.01441182191206211	0.0
24	8.78769628784275E-5	0.0	0.0	0.023785364619094376	0.0
25	8.78769628784275E-5	0.0	0.0	0.02853072061452946	0.0
26	8.78769628784275E-5	0.0	0.0	0.03597097013823632	0.0
27	8.78769628784275E-5	0.0	0.0	0.09285665744153838	0.0
28	8.78769628784275E-5	0.0	0.0	0.15094332990417897	0.0
29	8.78769628784275E-5	0.0	0.0	0.22051259218293406	0.0
30	8.78769628784275E-5	0.0	0.0	0.3460887721362069	0.0
31	8.78769628784275E-5	0.0	0.0	0.5352292885715424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8960	0.0	27.688057	1
GTATTGG	1015	0.0	18.773397	1
ATTGGAC	1010	0.0	17.950495	3
GGACCCT	1720	0.0	16.456396	6
TTGGACC	1815	0.0	16.410467	4
GTATCAA	15200	0.0	16.333553	2
CCCGTAT	145	5.3552867E-8	15.310345	1
GTATACT	355	0.0	15.112676	4
TGGACCC	1800	0.0	15.108334	5
TCTAACG	140	6.0043385E-7	14.535714	2
CGTCTGT	780	0.0	14.467948	34
CGTTTAG	275	0.0	14.127272	26
TATTGGA	1600	0.0	14.106251	2
TATACTG	460	0.0	14.076087	5
GACCCTC	1830	0.0	14.051913	7
GCGGTTA	95	0.0012459534	13.631579	30
AATGCGT	290	0.0	13.396551	35
ATTCGTC	180	5.1684765E-8	13.361111	17
CGAATTA	280	0.0	13.214285	15
TGCGTCT	850	0.0	12.841177	32
>>END_MODULE