FastQCFastQC Report
Wed 25 May 2016
SRR2088375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551615
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347090.9772737191390395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339090.9547487551437867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302740.8524009499903565No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG159040.44779628422562695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.3303567531953773No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA91340.25717877641579956No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81180.22857207214182843No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT80260.22598170128237438No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT78170.22009705443861455No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67710.19064566401482144No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62250.17527237608806134No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG57780.1626865524557138No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT53010.1492560426735443No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51950.14627148494417327No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47950.13500900294654686No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.12093090044951382No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA42110.11856577923001226No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40340.11358213094606256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86150.027.594311
ATTGGAC10250.021.8390243
GTATTGG11200.019.9866071
TTGGACC17050.016.9266874
GTATCAA141500.016.8395772
GGACCCT17550.015.9173796
TATTGGA15350.015.5472312
TGGACCC17900.015.399445
CTAGTAC3850.015.3766233
ACGGACC4400.014.7159098
TAATACT5650.014.407084
GACGGAC4550.014.230777
GACGTAT1051.6571696E-414.09523828
GTCGACG1601.7865568E-713.87536
AAGACCG2850.013.6315785
GACCGTG3000.013.5666677
GACCCTC19400.013.5412377
TAGTAGT3150.013.5079374
GAACCGT1951.0280928E-813.2820526
TATACCG2254.0745363E-1013.1555565
Produced by FastQC (version 0.11.5)