##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3551615
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32569549345861	31.0	31.0	34.0	30.0	34.0
2	31.529364528531385	31.0	31.0	34.0	30.0	34.0
3	31.61137820400015	31.0	31.0	34.0	30.0	34.0
4	35.41274631400081	37.0	35.0	37.0	33.0	37.0
5	35.225502201111325	37.0	35.0	37.0	33.0	37.0
6	35.25397910528027	37.0	35.0	37.0	32.0	37.0
7	35.134868503483624	37.0	35.0	37.0	32.0	37.0
8	35.1610881246982	37.0	35.0	37.0	32.0	37.0
9	36.669289604869896	39.0	35.0	39.0	32.0	39.0
10	36.51612745187752	38.0	35.0	39.0	32.0	39.0
11	36.62619653312648	39.0	35.0	39.0	32.0	39.0
12	36.4804104611564	38.0	35.0	39.0	32.0	39.0
13	36.52303585833487	38.0	35.0	39.0	32.0	39.0
14	37.530228924024705	39.0	36.0	41.0	32.0	41.0
15	37.5200603105911	39.0	36.0	41.0	32.0	41.0
16	37.47342040170458	39.0	36.0	41.0	32.0	41.0
17	37.510285884027404	39.0	36.0	41.0	32.0	41.0
18	37.48578379131747	39.0	36.0	41.0	32.0	41.0
19	37.538671562092176	39.0	36.0	41.0	32.0	41.0
20	37.493398918520164	39.0	36.0	41.0	32.0	41.0
21	37.44306885740712	39.0	36.0	41.0	32.0	41.0
22	37.332616851770254	39.0	36.0	41.0	31.0	41.0
23	37.22324660752925	39.0	36.0	41.0	31.0	41.0
24	37.1477938909482	39.0	36.0	41.0	31.0	41.0
25	37.0799351844161	39.0	36.0	41.0	31.0	41.0
26	36.858040919412716	39.0	36.0	40.0	31.0	41.0
27	36.696548471610804	39.0	35.0	40.0	30.0	41.0
28	36.57881358199017	38.0	35.0	40.0	30.0	41.0
29	36.43055201647701	38.0	35.0	40.0	30.0	41.0
30	36.25328589951332	38.0	35.0	40.0	30.0	41.0
31	36.04050241932191	38.0	35.0	40.0	29.0	41.0
32	35.8996605769488	38.0	35.0	40.0	29.0	41.0
33	35.99364514453284	38.0	35.0	40.0	29.0	41.0
34	35.98472075379792	38.0	35.0	40.0	29.0	41.0
35	35.91317555534595	38.0	35.0	40.0	29.0	41.0
36	35.8405677417175	38.0	35.0	40.0	28.0	41.0
37	35.7243316068887	38.0	34.0	40.0	28.0	41.0
38	35.61848877200936	38.0	34.0	40.0	27.0	41.0
39	35.563678777119705	38.0	34.0	40.0	27.0	41.0
40	35.430262007565574	38.0	34.0	40.0	26.0	41.0
41	35.30430522452462	38.0	34.0	40.0	26.0	41.0
42	35.15003118299703	38.0	34.0	40.0	25.0	41.0
43	34.81785891770364	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	18.0
16	43.0
17	109.0
18	269.0
19	553.0
20	1241.0
21	2430.0
22	4421.0
23	7508.0
24	12320.0
25	19908.0
26	29786.0
27	42766.0
28	59944.0
29	81431.0
30	103903.0
31	128666.0
32	156519.0
33	191621.0
34	233394.0
35	284832.0
36	359994.0
37	484559.0
38	651510.0
39	693861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43881023140177	21.886803609062355	13.274552562707386	23.399833596828483
2	18.550912753775396	22.806638670013502	35.19534071119758	23.44710786501352
3	20.008981829393107	25.68383678974213	31.428575450886427	22.878605929978335
4	13.431804967599248	16.138292016448855	35.4439036888852	34.9859993270667
5	12.521514860141092	39.26686873436451	34.30661825676488	13.904998148729522
6	32.16975939115022	36.511953012925105	15.630044360100968	15.688243235823704
7	26.004282558779597	33.78992373891877	21.677743786981416	18.528049915320214
8	27.711815610644734	33.29468424927814	20.16195449112587	18.831545648951252
9	26.0374787244676	13.616622297180298	22.255114926589734	38.09078405176237
10	16.52915645417648	27.087395452491332	33.70221716036226	22.68123093296993
11	33.21134188249571	21.86458836332204	23.926129380577567	20.997940373604685
12	20.77528110451161	26.778718977141384	31.116998886422092	21.329001031924914
13	30.73936786504168	20.69483882684356	27.46007098179279	21.105722326321967
14	21.50666668543747	21.971919816759417	27.474768520799692	29.04664497700342
15	23.637753529028345	28.76711017382233	25.336107658065416	22.259028639083912
16	22.909352505831855	27.670876488583364	26.191352384760176	23.228418620824613
17	20.89612753634614	26.83643919737922	27.16882319733417	25.09861006894047
18	21.833222350958646	25.82315369205277	30.441615997229434	21.90200795975915
19	21.47454045553924	24.922774568752526	31.253922511308236	22.3487624644
20	24.34174312249498	24.156756855683962	30.284250967517597	21.217249054303462
21	24.203524312179105	24.966557467518296	29.336062608137425	21.49385561216517
22	23.047036348252835	24.67629515023447	30.09858895178672	22.17807954972597
23	22.126722631816794	25.783988410906023	30.784502261647166	21.304786695630014
24	21.277756738835713	25.440567178593398	29.708878918463853	23.572797164107033
25	22.226114035445846	25.866457935333642	30.334819511686938	21.572608517533574
26	23.002155357492295	25.4785498991304	29.49537041599385	22.023924327383458
27	22.219722576912194	26.311607536289827	29.203672132255328	22.26499775454265
28	21.892603787291133	25.923164532191695	29.529889923316578	22.654341757200598
29	21.168144632793815	25.918772164212616	30.195699702811257	22.71738350018231
30	20.83052357870997	26.380590238525294	31.119870819331485	21.669015363433257
31	21.911637381867124	25.853083737961462	30.303453499323545	21.93182538084787
32	22.48748808640576	25.88580124816457	29.033327092041226	22.593383573388444
33	22.882998297957407	25.771121025223735	29.013561436135394	22.33231924068346
34	22.084094137455775	27.455932019658665	29.018770334059298	21.441203508826266
35	21.232875748075173	28.1632158891096	29.32474381372981	21.279164549085415
36	21.58941777191503	26.030439673219085	30.411404389270796	21.968738165595088
37	21.826830892424994	26.041251655936804	29.864554575876046	22.267362875762153
38	21.788791859478014	25.634366337567556	30.410897577580904	22.165944225373526
39	22.514855917659997	25.303559085092274	30.333580638667197	21.848004358580532
40	21.36664587800198	25.791027462154542	29.94719303753363	22.895133622309853
41	21.726735583671093	25.495950433816727	30.029183906476348	22.74813007603583
42	20.314673747013682	26.639993355135623	30.34121097022059	22.704121927630105
43	20.562251257526505	26.161168933006536	30.013331963064687	23.263247846402273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	391.5
2	511.0
3	1444.5
4	2378.0
5	2378.0
6	3960.5
7	5543.0
8	5841.0
9	6139.0
10	9236.0
11	12333.0
12	12333.0
13	21927.0
14	31521.0
15	51229.5
16	70938.0
17	69666.0
18	68394.0
19	68394.0
20	73721.5
21	79049.0
22	65735.0
23	52421.0
24	58829.5
25	65238.0
26	65238.0
27	72463.5
28	79689.0
29	85723.5
30	91758.0
31	98680.0
32	105602.0
33	105602.0
34	116264.5
35	126927.0
36	140694.5
37	154462.0
38	163482.0
39	172502.0
40	172502.0
41	182905.5
42	193309.0
43	210735.5
44	228162.0
45	268397.5
46	308633.0
47	308633.0
48	314813.5
49	320994.0
50	298537.5
51	276081.0
52	264825.0
53	253569.0
54	253569.0
55	228697.5
56	203826.0
57	180950.5
58	158075.0
59	144314.0
60	130553.0
61	130553.0
62	117037.0
63	103521.0
64	91729.0
65	79937.0
66	68887.0
67	57837.0
68	57837.0
69	49552.5
70	41268.0
71	35463.5
72	29659.0
73	23251.0
74	16843.0
75	16843.0
76	13045.0
77	9247.0
78	7563.5
79	5880.0
80	4827.5
81	3775.0
82	3775.0
83	2840.5
84	1906.0
85	1573.0
86	1240.0
87	1084.5
88	929.0
89	929.0
90	736.0
91	543.0
92	319.5
93	96.0
94	67.0
95	38.0
96	38.0
97	26.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3551615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.78163129580517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6597583008453	31.512846209648878
2	13.480193677248497	11.534093513921787
3	5.1913341260992505	6.662812275483272
4	2.4247944495438425	4.149466484339982
5	1.339689563227006	2.8657052472408022
6	0.8091481194392884	2.0770005905727453
7	0.5540912174277357	1.6593448317766042
8	0.39239567125116454	1.342986154362984
9	0.2906303428151914	1.1190276152723677
>10	1.596872922459733	12.783791531435943
>50	0.14614794404459977	4.381161568281377
>100	0.10091207395008074	8.392424830696559
>500	0.008629758244883915	2.5628624447069828
>1k	0.004479569163877024	3.6329083140751735
>5k	5.928841540425473E-4	1.7576224894868173
>10k+	3.29380085579193E-4	3.565945898697627
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34709	0.9772737191390395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33909	0.9547487551437867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30274	0.8524009499903565	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15904	0.44779628422562695	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11733	0.3303567531953773	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9134	0.25717877641579956	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8118	0.22857207214182843	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8026	0.22598170128237438	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7817	0.22009705443861455	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6771	0.19064566401482144	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6225	0.17527237608806134	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5778	0.1626865524557138	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5301	0.1492560426735443	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5195	0.14627148494417327	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4795	0.13500900294654686	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4295	0.12093090044951382	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4211	0.11856577923001226	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4034	0.11358213094606256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.815620499406608E-5	0.0
6	0.0	0.0	0.0	5.631240998813216E-5	0.0
7	0.0	0.0	0.0	5.631240998813216E-5	0.0
8	0.0	0.0	0.0	5.631240998813216E-5	0.0
9	0.0	0.0	0.0	8.446861498219824E-5	0.0
10	0.0	0.0	0.0	1.1262481997626432E-4	0.0
11	0.0	0.0	0.0	2.2524963995252864E-4	0.0
12	0.0	0.0	0.0	2.815620499406608E-4	0.0
13	0.0	0.0	0.0	3.3787445992879297E-4	0.0
14	0.0	0.0	0.0	4.504992799050573E-4	0.0
15	0.0	0.0	0.0	7.602175348397841E-4	0.0
16	0.0	0.0	0.0	0.001379654044709238	0.0
17	0.0	0.0	0.0	0.0017175285046380309	0.0
18	0.0	0.0	0.0	0.0019709343495846257	0.0
19	0.0	0.0	0.0	0.0022524963995252862	0.0
20	0.0	0.0	0.0	0.0025903708594540793	0.0
21	0.0	0.0	0.0	0.0036039942392404583	0.0
22	0.0	0.0	0.0	0.006391458533653	0.0
23	0.0	0.0	0.0	0.010474108257792582	0.0
24	0.0	0.0	0.0	0.017287909866356574	0.0
25	0.0	0.0	0.0	0.02159580923044868	0.0
26	0.0	0.0	0.0	0.029282453193828724	0.0
27	0.0	0.0	0.0	0.0850035828770855	0.0
28	0.0	0.0	0.0	0.1461870163291911	0.0
29	0.0	0.0	0.0	0.21502893753968266	0.0
30	0.0	0.0	0.0	0.3355093387092914	0.0
31	0.0	0.0	0.0	0.5266899706190001	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8615	0.0	27.59431	1
ATTGGAC	1025	0.0	21.839024	3
GTATTGG	1120	0.0	19.986607	1
TTGGACC	1705	0.0	16.926687	4
GTATCAA	14150	0.0	16.839577	2
GGACCCT	1755	0.0	15.917379	6
TATTGGA	1535	0.0	15.547231	2
TGGACCC	1790	0.0	15.39944	5
CTAGTAC	385	0.0	15.376623	3
ACGGACC	440	0.0	14.715909	8
TAATACT	565	0.0	14.40708	4
GACGGAC	455	0.0	14.23077	7
GACGTAT	105	1.6571696E-4	14.095238	28
GTCGACG	160	1.7865568E-7	13.875	36
AAGACCG	285	0.0	13.631578	5
GACCGTG	300	0.0	13.566667	7
GACCCTC	1940	0.0	13.541237	7
TAGTAGT	315	0.0	13.507937	4
GAACCGT	195	1.0280928E-8	13.282052	6
TATACCG	225	4.0745363E-10	13.155556	5
>>END_MODULE