FastQCFastQC Report
Wed 25 May 2016
SRR2088374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993804
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT303211.0127917525663004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289010.9653604577988405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263020.8785478274462858No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG148430.49579063960098924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.32928007311099855No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83470.2788091671999904No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA75420.2519202993916769No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT74820.24991616017615048No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT74610.24921471145071622No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA61360.20495663710784007No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT59120.1974745173698746No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG54900.18337873822067174No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT51260.17122029364647787No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48120.16073196508522267No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45210.15101188988991932No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39020.13033585364973793No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38080.12719603554541314No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT37080.12385580351953569No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34630.11567223505613594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70550.028.8972381
GTATTGG9600.020.4270821
ATTGGAC9550.019.9528793
TTGGACC15150.017.584164
ACGGACC3300.017.3787888
GTATCAA119950.016.9962482
GGACCCT15800.016.9778486
CGAATTA2150.016.34883715
TAAACGT1251.6602098E-716.2799994
TGGACCC16550.016.0966785
TCTTACG700.002593797415.85714226
CGAACGA2150.015.48837216
ACGTTTA1201.9379295E-615.41666626
TATTGGA13650.015.0439562
TCCGATA2550.014.5098048
GATAACG2301.8189894E-1214.4782611
GACCCTC17550.014.4415957
CGTCTGT7200.014.38888734
CTAGTAC3350.014.3582093
TAACGAA2353.6379788E-1214.17021413
Produced by FastQC (version 0.11.5)