##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2993804
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429459978007912	31.0	31.0	34.0	30.0	34.0
2	31.627897484270846	31.0	31.0	34.0	30.0	34.0
3	31.715051486336446	31.0	31.0	34.0	30.0	34.0
4	35.49870231985795	37.0	35.0	37.0	33.0	37.0
5	35.32610651866321	37.0	35.0	37.0	33.0	37.0
6	35.35234437524968	37.0	35.0	37.0	32.0	37.0
7	35.24094997534909	37.0	35.0	37.0	32.0	37.0
8	35.26690057198133	37.0	35.0	37.0	32.0	37.0
9	36.78750512725616	39.0	37.0	39.0	32.0	39.0
10	36.65047845483539	39.0	35.0	39.0	32.0	39.0
11	36.754060386050654	39.0	35.0	39.0	32.0	39.0
12	36.61807920625398	39.0	35.0	39.0	32.0	39.0
13	36.65502751683143	39.0	35.0	39.0	32.0	39.0
14	37.68651220988415	40.0	37.0	41.0	32.0	41.0
15	37.679776297980766	39.0	36.0	41.0	32.0	41.0
16	37.631840294154195	39.0	36.0	41.0	32.0	41.0
17	37.68284530316614	39.0	37.0	41.0	32.0	41.0
18	37.660991835136834	39.0	37.0	41.0	32.0	41.0
19	37.71215650723962	40.0	37.0	41.0	32.0	41.0
20	37.67062205809064	39.0	37.0	41.0	32.0	41.0
21	37.61900812478038	39.0	37.0	41.0	32.0	41.0
22	37.51616872714446	39.0	36.0	41.0	32.0	41.0
23	37.40957457468826	39.0	36.0	41.0	32.0	41.0
24	37.34370853936998	39.0	36.0	41.0	31.0	41.0
25	37.27964121899764	39.0	36.0	41.0	31.0	41.0
26	37.064822880856596	39.0	36.0	41.0	31.0	41.0
27	36.90938985985723	39.0	35.0	40.0	31.0	41.0
28	36.79496653755557	39.0	35.0	40.0	30.0	41.0
29	36.66261685801742	39.0	35.0	40.0	30.0	41.0
30	36.483839957458805	38.0	35.0	40.0	30.0	41.0
31	36.263236337448944	38.0	35.0	40.0	30.0	41.0
32	36.13573734285878	38.0	35.0	40.0	30.0	41.0
33	36.22402468565076	38.0	35.0	40.0	30.0	41.0
34	36.20006720546836	38.0	35.0	40.0	30.0	41.0
35	36.133053132402786	38.0	35.0	40.0	30.0	41.0
36	36.058712260388454	38.0	35.0	40.0	29.0	41.0
37	35.942517947066676	38.0	35.0	40.0	29.0	41.0
38	35.83389226549233	38.0	35.0	40.0	28.0	41.0
39	35.789897401433095	38.0	35.0	40.0	27.0	41.0
40	35.65728016930968	38.0	35.0	40.0	27.0	41.0
41	35.53436397305902	38.0	35.0	40.0	26.0	41.0
42	35.38478170247618	38.0	34.0	40.0	26.0	41.0
43	35.06345371974919	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	0.0
14	4.0
15	11.0
16	25.0
17	63.0
18	158.0
19	462.0
20	815.0
21	1673.0
22	3044.0
23	5355.0
24	9155.0
25	14525.0
26	22406.0
27	33241.0
28	46547.0
29	63631.0
30	82158.0
31	102362.0
32	124859.0
33	154132.0
34	189823.0
35	232737.0
36	297188.0
37	406921.0
38	552913.0
39	649591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39088597650347	22.01196203893107	13.341120527596328	23.256031456969126
2	18.104057580255756	22.78058283040573	35.96658298272031	23.148776606618203
3	19.784027277670816	25.85666930767679	31.653909207149166	22.70539420750323
4	13.136197292808749	16.01096798588017	35.85982916717327	34.99300555413781
5	12.2353367154296	39.4625032233239	34.58646591426827	13.715694146978224
6	31.734542408253848	36.61756080224357	15.759548721292377	15.88834806821021
7	25.543856578453365	34.0481207186576	21.918268530605207	18.489754172283824
8	27.284217670896293	33.63874188156606	20.415063911999585	18.661976535538063
9	25.771760609578987	13.78373467334535	22.629337124274002	37.81516759280167
10	16.367504352322328	27.197104419661404	34.01431757055572	22.421073657460543
11	32.59244760178021	22.039285136902752	24.324772095968875	21.043495165348165
12	20.481901954837394	26.91926391974892	31.44564574033571	21.153188385077982
13	30.571573823804098	20.849661500886498	27.820258106409103	20.7585065689003
14	21.226840501248578	22.13357988699327	27.875004509313232	28.764575102444915
15	23.383127285553766	28.81578085940162	25.8411038264362	21.959988028608418
16	22.54082097558825	28.03296408181698	26.43446264351307	22.991752299081703
17	20.445493425755327	26.956206885955126	27.526384492772404	25.071915195517143
18	21.461825824269056	26.19894288336845	30.836086797933334	21.50314449442916
19	20.987145451071612	25.08587736538531	31.752312442631514	22.174664740911563
20	24.204623949998062	24.32163227786455	30.705750944283594	20.767992827853792
21	23.856838991463704	25.160130723320567	29.740056463282166	21.242973821933568
22	22.637687704338695	24.79778235315338	30.61088835474867	21.95364158775925
23	21.7931768412361	26.059220977725992	31.341564110409365	20.80603807062854
24	20.806906530955267	25.541652025316285	30.22011461004127	23.431326833687173
25	21.885133428908507	26.125491181119404	30.83441668192039	21.154958708051698
26	22.737326825670618	25.670017142070755	29.83505266209812	21.757603370160506
27	21.779515292250263	26.61059307823759	29.651440107635636	21.958451521876515
28	21.540722104720285	26.097299622821	29.9059657880075	22.45601248445122
29	20.69013201933059	26.15638832735877	30.605610788147786	22.547868865162847
30	20.415264325921136	26.52722088687168	31.73915192844956	21.31836285875762
31	21.459988696654825	26.019772837500383	30.909338086260824	21.610900379583967
32	22.223732749371703	25.999297215181755	29.48730110588402	22.289668929562524
33	22.764349302759967	25.86515349702252	29.354126055012287	22.01637114520523
34	21.724969303267684	27.75275201716612	29.393240172035313	21.129038507530886
35	20.799023583374197	28.494684354754018	29.640851572113608	21.06544048975818
36	21.32784911771111	26.031797672793545	30.897747481131027	21.742605728364314
37	21.535811963642242	26.040716092302635	30.383485358426938	22.039986585628185
38	21.379856530354026	25.718584115727015	30.98716549246377	21.914393861455192
39	22.197712341890117	25.327008715333406	30.926239660311765	21.549039282464715
40	20.998268423717786	25.817822409215836	30.444945627703085	22.7389635393633
41	21.50177499929855	25.480191756040142	30.43846557757288	22.579567667088426
42	19.759309560679323	26.851791232826194	30.742192875685916	22.646706330808563
43	20.033609414644378	26.430187146519945	30.49681943106496	23.039384007770717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	343.5
2	455.0
3	1278.5
4	2102.0
5	2102.0
6	3509.5
7	4917.0
8	5243.0
9	5569.0
10	8243.5
11	10918.0
12	10918.0
13	19014.5
14	27111.0
15	44011.5
16	60912.0
17	60269.5
18	59627.0
19	59627.0
20	64830.5
21	70034.0
22	58503.5
23	46973.0
24	52679.0
25	58385.0
26	58385.0
27	65307.0
28	72229.0
29	76382.0
30	80535.0
31	87163.5
32	93792.0
33	93792.0
34	102840.5
35	111889.0
36	121973.5
37	132058.0
38	138618.0
39	145178.0
40	145178.0
41	153560.5
42	161943.0
43	178283.5
44	194624.0
45	231299.0
46	267974.0
47	267974.0
48	272816.5
49	277659.0
50	257269.5
51	236880.0
52	226334.5
53	215789.0
54	215789.0
55	192754.0
56	169719.0
57	147972.5
58	126226.0
59	113787.0
60	101348.0
61	101348.0
62	90335.0
63	79322.0
64	69984.5
65	60647.0
66	51612.0
67	42577.0
68	42577.0
69	35749.0
70	28921.0
71	24662.5
72	20404.0
73	15999.5
74	11595.0
75	11595.0
76	8983.5
77	6372.0
78	5056.0
79	3740.0
80	3018.5
81	2297.0
82	2297.0
83	1721.5
84	1146.0
85	964.5
86	783.0
87	643.0
88	503.0
89	503.0
90	411.5
91	320.0
92	189.5
93	59.0
94	43.0
95	27.0
96	27.0
97	19.0
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2993804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.59012553830597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0525657488183	32.27960637424646
2	13.665521564014934	11.913636010436766
3	4.990252912978692	6.525772528339147
4	2.3671324347280187	4.127343999823608
5	1.2646741602590394	2.7563652705371604
6	0.7603020406211035	1.9884996838622464
7	0.5152034320990605	1.5720447598074905
8	0.36039444599991727	1.25677113155557
9	0.25764002066431896	1.0107504760004467
>10	1.5063099485267148	12.465293029871518
>50	0.1468668922118322	4.494345396490545
>100	0.10010824422983959	8.207865015989
>500	0.007203803269046992	2.055560196707326
>1k	0.004828080914336334	3.87691220366133
>5k	6.897258449051906E-4	2.116392191208995
>10k+	3.0654481995786247E-4	3.3528417314624677
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30321	1.0127917525663004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28901	0.9653604577988405	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26302	0.8785478274462858	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14843	0.49579063960098924	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9858	0.32928007311099855	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8347	0.2788091671999904	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7542	0.2519202993916769	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7482	0.24991616017615048	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7461	0.24921471145071622	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6136	0.20495663710784007	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5912	0.1974745173698746	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5490	0.18337873822067174	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5126	0.17122029364647787	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4812	0.16073196508522267	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4521	0.15101188988991932	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3902	0.13033585364973793	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3808	0.12719603554541314	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3708	0.12385580351953569	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3463	0.11567223505613594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.340232025877445E-5	0.0
11	0.0	0.0	0.0	2.338162418114212E-4	0.0
12	0.0	0.0	0.0	3.3402320258774456E-4	0.0
13	0.0	0.0	0.0	4.676324836228424E-4	0.0
14	0.0	0.0	0.0	5.344371241403912E-4	0.0
15	0.0	0.0	0.0	7.682533659518125E-4	0.0
16	0.0	0.0	0.0	0.0014028974508685271	0.0
17	0.0	0.0	0.0	0.0024383693788905354	0.0
18	0.0	0.0	0.0	0.00277239258147828	0.0
19	0.0	0.0	0.0	0.0033402320258774454	0.0
20	0.0	0.0	0.0	0.0041752900323468066	0.0
21	0.0	0.0	0.0	0.00584540604528553	0.0
22	0.0	0.0	0.0	0.009452856633233172	0.0
23	0.0	0.0	0.0	0.01583269980265909	0.0
24	0.0	0.0	0.0	0.026053809801844074	0.0
25	0.0	0.0	0.0	0.030696732317813724	0.0
26	0.0	0.0	0.0	0.039114117023024884	0.0
27	0.0	0.0	0.0	0.09997314453451195	0.0
28	0.0	0.0	0.0	0.16604293400636783	0.0
29	0.0	0.0	0.0	0.24584107710458	0.0
30	0.0	0.0	0.0	0.3846277177797879	0.0
31	0.0	0.0	0.0	0.6036467317165719	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7055	0.0	28.897238	1
GTATTGG	960	0.0	20.427082	1
ATTGGAC	955	0.0	19.952879	3
TTGGACC	1515	0.0	17.58416	4
ACGGACC	330	0.0	17.378788	8
GTATCAA	11995	0.0	16.996248	2
GGACCCT	1580	0.0	16.977848	6
CGAATTA	215	0.0	16.348837	15
TAAACGT	125	1.6602098E-7	16.279999	4
TGGACCC	1655	0.0	16.096678	5
TCTTACG	70	0.0025937974	15.857142	26
CGAACGA	215	0.0	15.488372	16
ACGTTTA	120	1.9379295E-6	15.416666	26
TATTGGA	1365	0.0	15.043956	2
TCCGATA	255	0.0	14.509804	8
GATAACG	230	1.8189894E-12	14.47826	11
GACCCTC	1755	0.0	14.441595	7
CGTCTGT	720	0.0	14.388887	34
CTAGTAC	335	0.0	14.358209	3
TAACGAA	235	3.6379788E-12	14.170214	13
>>END_MODULE