FastQCFastQC Report
Wed 25 May 2016
SRR2088373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729950
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT355551.302404805948827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT346201.2681550944156488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314221.1510100917599222No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG165320.6055788567556182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113130.41440319419769595No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA96870.35484166376673565No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86340.3162695287459477No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT85240.31224015091851504No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85080.3116540595981611No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70500.2582464880309163No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT68880.25231231341233357No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG60650.22216524112163225No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT57370.21015036905437828No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55370.2028242275499551No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51250.18773237605084342No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45680.16732907196102492No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43930.16091869814465468No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.15699921243978826No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38550.14121137749775636No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34690.1270719243942197No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32420.11875675378669938No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31860.11670543416546089No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31460.11524020586457628No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.1111009359145772No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29440.10784080294510888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83000.027.6831321
ATTGGAC10900.019.6880743
GTATTGG10850.019.6082951
ATAACGC2050.017.1463413
ACGTTTA1655.4569682E-1116.81818226
TTGGACC17350.016.314124
GTATCAA141950.016.2257842
CGTCTGT6750.016.1703734
GGACCCT16400.015.9054876
TAAGACG700.00259371915.8571424
TATTGGA13700.015.6642332
CGCCGTT1557.221388E-915.51612925
TGGACCC17600.015.4517045
CGGAATA855.367507E-415.23529427
CGCGTAT1101.4532443E-515.13636424
TATACCG2151.2732926E-1114.6279075
CAATGCG1406.003065E-714.53571419
TAGTGCG908.2791556E-414.3888887
ACCGTTA1551.2119744E-714.322588
GTGATCG1051.6569474E-414.0952388
Produced by FastQC (version 0.11.5)