##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2729950
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420880968515906	31.0	31.0	34.0	30.0	34.0
2	31.6122137768091	31.0	31.0	34.0	30.0	34.0
3	31.690532427333835	31.0	31.0	34.0	30.0	34.0
4	35.48140149086979	37.0	35.0	37.0	33.0	37.0
5	35.32017362955366	37.0	35.0	37.0	33.0	37.0
6	35.351389952196925	37.0	35.0	37.0	32.0	37.0
7	35.23173501346179	37.0	35.0	37.0	32.0	37.0
8	35.26955621897837	37.0	35.0	37.0	32.0	37.0
9	36.768963167823586	39.0	37.0	39.0	32.0	39.0
10	36.65049543031924	39.0	35.0	39.0	32.0	39.0
11	36.74839429293577	39.0	35.0	39.0	32.0	39.0
12	36.62390300188648	39.0	35.0	39.0	32.0	39.0
13	36.661900401106244	39.0	35.0	39.0	32.0	39.0
14	37.668134214912364	39.0	37.0	41.0	32.0	41.0
15	37.65775270609352	39.0	36.0	41.0	32.0	41.0
16	37.6051297642814	39.0	36.0	41.0	32.0	41.0
17	37.69328522500412	39.0	37.0	41.0	32.0	41.0
18	37.67410575285262	39.0	37.0	41.0	32.0	41.0
19	37.7416513122951	40.0	37.0	41.0	32.0	41.0
20	37.70545101558637	39.0	37.0	41.0	32.0	41.0
21	37.65475338376161	39.0	37.0	41.0	32.0	41.0
22	37.549556585285444	39.0	36.0	41.0	32.0	41.0
23	37.456166230150735	39.0	36.0	41.0	32.0	41.0
24	37.39084268942655	39.0	36.0	41.0	32.0	41.0
25	37.332779354933244	39.0	36.0	41.0	31.0	41.0
26	37.10723273320024	39.0	36.0	41.0	31.0	41.0
27	36.94287697576879	39.0	35.0	40.0	31.0	41.0
28	36.82679756039488	39.0	35.0	40.0	31.0	41.0
29	36.68417443542922	39.0	35.0	40.0	30.0	41.0
30	36.49406033077529	38.0	35.0	40.0	30.0	41.0
31	36.24147731643437	38.0	35.0	40.0	30.0	41.0
32	36.106473012326234	38.0	35.0	40.0	30.0	41.0
33	36.141394897342444	38.0	35.0	40.0	30.0	41.0
34	36.10250334255206	38.0	35.0	40.0	30.0	41.0
35	35.99871169801645	38.0	35.0	40.0	30.0	41.0
36	35.907368266818075	38.0	35.0	40.0	29.0	41.0
37	35.77104379201084	38.0	35.0	40.0	28.0	41.0
38	35.64225205589846	38.0	35.0	40.0	27.0	41.0
39	35.55428451070532	38.0	35.0	40.0	27.0	41.0
40	35.38424952837964	38.0	34.0	40.0	26.0	41.0
41	35.23226652502793	38.0	34.0	40.0	25.0	41.0
42	35.053873880474	38.0	34.0	40.0	24.0	41.0
43	34.71476693712339	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	4.0
15	4.0
16	20.0
17	64.0
18	139.0
19	397.0
20	759.0
21	1606.0
22	3090.0
23	5306.0
24	8780.0
25	13829.0
26	21133.0
27	31046.0
28	44683.0
29	60073.0
30	76933.0
31	94755.0
32	116257.0
33	141759.0
34	174663.0
35	213287.0
36	270404.0
37	371201.0
38	483493.0
39	596262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48808586237843	22.429678199234417	13.736405428670857	22.345830509716293
2	17.84160882067437	23.060532244180294	36.44509972710123	22.652759208044102
3	19.810289565742963	26.291287386215867	32.1063389439367	21.792084104104468
4	12.77272477517903	15.929412626604883	35.61878422681734	35.679078371398745
5	11.63486510741955	40.285829410795074	34.39894503562336	13.680360446162018
6	31.213721863037787	36.379933698419386	15.860437004340739	16.545907434202093
7	24.758878367735672	35.01972563600066	21.71332808293192	18.508067913331747
8	27.634169123976633	33.365116577226686	20.383743292001686	18.616971006794998
9	25.607941537390793	13.779886078499606	23.479441015403214	37.13273136870639
10	16.59554204289456	27.445374457407645	33.49028370482976	22.468799794868037
11	31.704573343834134	22.085605963479185	24.88748878184582	21.32233191084086
12	20.49484422791626	27.49314090001648	31.6606164948076	20.35139837725966
13	30.934852286671916	21.055880144324988	28.40425648821407	19.605011080789026
14	21.072583746955072	22.492756277587503	28.526383267092804	27.90827670836462
15	23.149178556383816	28.90507152145644	26.796351581530796	21.14939834062895
16	21.82831187384384	28.21469257678712	27.21569992124398	22.74129562812506
17	19.64911445264565	27.056795912013044	27.945420245792047	25.34866938954926
18	20.720306232714886	26.26678144288357	32.07875602117255	20.934156303228995
19	20.084873349328742	24.81305518416088	33.41870730233155	21.68336416417883
20	24.17278704738182	23.893661056063298	32.06271177127786	19.87084012527702
21	23.648967929815566	24.808293192183008	31.03250975292588	20.51022912507555
22	22.170735727760583	24.42469642301141	31.950218868477442	21.454348980750563
23	21.129434605029395	26.090148171211926	32.84298247220645	19.937434751552228
24	19.845125368596495	25.544204106302317	31.452957013864726	23.15771351123647
25	21.06185094965109	26.307697943185772	32.31835747907471	20.312093628088427
26	22.362973680836646	25.420612099122696	31.139984248795766	21.076429971244895
27	20.874924449165736	26.79045403761974	30.816351947837873	21.518269565376656
28	20.73876810930603	26.249894686715873	30.95133610505687	22.060001098921227
29	19.816040586823934	26.25916225571897	31.891499844319494	22.033297313137602
30	19.357607282184656	26.600853495485268	33.407754720782435	20.633784501547648
31	20.684151724390556	26.007142987966812	32.446418432572024	20.862286855070604
32	21.61603692375318	26.090477847579624	30.640158244656497	21.653326984010697
33	22.337625231231343	26.070917049762816	30.256378321947285	21.335079397058553
34	20.960676935475007	28.425209252916723	30.278613161413215	20.33550065019506
35	19.754903935969523	29.31617795197714	30.544918405098993	20.383999706954338
36	20.47872671660653	26.12948955109068	32.37817542445832	21.013608307844468
37	20.617520467407825	26.411839044671147	31.51383724976648	21.456803238154546
38	20.821077309108222	25.936921921646917	32.060843605194236	21.181157164050624
39	21.70428029817396	25.381087565706334	32.12033187421015	20.79430026190956
40	20.06644810344512	26.170259528562795	31.34053737247935	22.42275499551274
41	20.830051832451144	25.736112383010678	31.350024725727575	22.0838110588106
42	18.638546493525524	27.301818714628475	31.803989084049157	22.255645707796845
43	19.233832121467426	27.027051777505086	31.289181120533343	22.44993498049415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	269.0
1	350.5
2	432.0
3	1386.0
4	2340.0
5	2340.0
6	3912.5
7	5485.0
8	5841.5
9	6198.0
10	9185.0
11	12172.0
12	12172.0
13	21615.0
14	31058.0
15	51215.0
16	71372.0
17	69736.5
18	68101.0
19	68101.0
20	71809.5
21	75518.0
22	60697.0
23	45876.0
24	50872.0
25	55868.0
26	55868.0
27	61194.5
28	66521.0
29	70196.0
30	73871.0
31	78967.0
32	84063.0
33	84063.0
34	91443.5
35	98824.0
36	107900.5
37	116977.0
38	122703.5
39	128430.0
40	128430.0
41	135402.5
42	142375.0
43	160302.0
44	178229.0
45	220120.0
46	262011.0
47	262011.0
48	265021.0
49	268031.0
50	244893.5
51	221756.0
52	209196.0
53	196636.0
54	196636.0
55	171343.0
56	146050.0
57	123184.0
58	100318.0
59	88902.0
60	77486.0
61	77486.0
62	68322.5
63	59159.0
64	51791.0
65	44423.0
66	37822.0
67	31221.0
68	31221.0
69	26571.0
70	21921.0
71	18583.0
72	15245.0
73	12325.0
74	9405.0
75	9405.0
76	7397.5
77	5390.0
78	4234.5
79	3079.0
80	2411.0
81	1743.0
82	1743.0
83	1318.0
84	893.0
85	713.0
86	533.0
87	440.0
88	347.0
89	347.0
90	292.0
91	237.0
92	148.0
93	59.0
94	39.0
95	19.0
96	19.0
97	12.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2729950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.94731874092385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3321019454833	31.180323729900334
2	13.504998513254549	11.329969544623824
3	4.947422001136155	6.225932628825528
4	2.3488835702553006	3.941174712272731
5	1.234114362149776	2.588389425592429
6	0.7363536310363431	1.8532836280270872
7	0.49912664803718015	1.4655917218113153
8	0.351252074560277	1.178726618399255
9	0.2610990602608034	0.9857164953344041
>10	1.5053451587214555	12.113388887547211
>50	0.15999257216286522	4.687411455691648
>100	0.10557666426082547	8.355112837069358
>500	0.006385779004620977	1.8527174962587207
>1k	0.006035873305737223	4.8595351428871485
>5k	8.747642472082933E-4	2.632979024856663
>10k+	4.3738212360414666E-4	4.749746650902323
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35555	1.302404805948827	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34620	1.2681550944156488	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31422	1.1510100917599222	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16532	0.6055788567556182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11313	0.41440319419769595	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9687	0.35484166376673565	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8634	0.3162695287459477	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8524	0.31224015091851504	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8508	0.3116540595981611	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7050	0.2582464880309163	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6888	0.25231231341233357	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6065	0.22216524112163225	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5737	0.21015036905437828	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5537	0.2028242275499551	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5125	0.18773237605084342	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4568	0.16732907196102492	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4393	0.16091869814465468	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4286	0.15699921243978826	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3855	0.14121137749775636	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3469	0.1270719243942197	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3242	0.11875675378669938	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3186	0.11670543416546089	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3146	0.11524020586457628	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3033	0.1111009359145772	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2944	0.10784080294510888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.663070752211579E-5	0.0
6	3.663070752211579E-5	0.0	0.0	3.663070752211579E-5	0.0
7	3.663070752211579E-5	0.0	0.0	3.663070752211579E-5	0.0
8	7.326141504423158E-5	0.0	0.0	3.663070752211579E-5	0.0
9	7.326141504423158E-5	0.0	0.0	3.663070752211579E-5	0.0
10	7.326141504423158E-5	0.0	0.0	7.326141504423158E-5	0.0
11	7.326141504423158E-5	0.0	0.0	2.564149526548105E-4	0.0
12	7.326141504423158E-5	0.0	0.0	6.593527353980842E-4	0.0
13	7.326141504423158E-5	0.0	0.0	7.692448579644316E-4	0.0
14	7.326141504423158E-5	0.0	0.0	9.890291030971263E-4	0.0
15	7.326141504423158E-5	0.0	0.0	0.0015384897159288632	0.0
16	7.326141504423158E-5	0.0	0.0	0.0023809959889375264	0.0
17	7.326141504423158E-5	0.0	0.0	0.0035531786296452315	0.0
18	7.326141504423158E-5	0.0	0.0	0.003882854997344274	0.0
19	7.326141504423158E-5	0.0	0.0	0.004725361270352937	0.0
20	7.326141504423158E-5	0.0	0.0	0.005787651788494295	0.0
21	7.326141504423158E-5	0.0	0.0	0.008168647777431821	0.0
22	7.326141504423158E-5	0.0	0.0	0.014579021593802085	0.0
23	7.326141504423158E-5	0.0	0.0	0.023700067766808917	0.0
24	7.326141504423158E-5	0.0	0.0	0.036264400446894635	0.0
25	7.326141504423158E-5	0.0	0.0	0.041356068792468725	0.0
26	7.326141504423158E-5	0.0	0.0	0.05066026850308614	0.0
27	7.326141504423158E-5	0.0	0.0	0.12234656312386674	0.0
28	7.326141504423158E-5	0.0	0.0	0.19894137255261085	0.0
29	7.326141504423158E-5	0.0	0.0	0.28879649810436087	0.0
30	7.326141504423158E-5	0.0	0.0	0.4693492554808696	0.0
31	7.326141504423158E-5	0.0	0.0	0.7288045568600158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8300	0.0	27.683132	1
ATTGGAC	1090	0.0	19.688074	3
GTATTGG	1085	0.0	19.608295	1
ATAACGC	205	0.0	17.146341	3
ACGTTTA	165	5.4569682E-11	16.818182	26
TTGGACC	1735	0.0	16.31412	4
GTATCAA	14195	0.0	16.225784	2
CGTCTGT	675	0.0	16.17037	34
GGACCCT	1640	0.0	15.905487	6
TAAGACG	70	0.002593719	15.857142	4
TATTGGA	1370	0.0	15.664233	2
CGCCGTT	155	7.221388E-9	15.516129	25
TGGACCC	1760	0.0	15.451704	5
CGGAATA	85	5.367507E-4	15.235294	27
CGCGTAT	110	1.4532443E-5	15.136364	24
TATACCG	215	1.2732926E-11	14.627907	5
CAATGCG	140	6.003065E-7	14.535714	19
TAGTGCG	90	8.2791556E-4	14.388888	7
ACCGTTA	155	1.2119744E-7	14.32258	8
GTGATCG	105	1.6569474E-4	14.095238	8
>>END_MODULE