FastQCFastQC Report
Wed 25 May 2016
SRR2088372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975757
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT440791.108694520313993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT431211.0845984802391093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT393540.9898492287129218No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG223630.5624840753597365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145940.3670747482806419No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA129350.3253468458962658No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA111390.28017305886652527No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT109270.2748407410211439No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT109150.2745389117091412No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA89640.2254664960660322No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT88020.22139180035399547No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG81350.2046151210951776No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT75760.1905549056443842No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA75530.18997639946304568No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68060.17118752479087632No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56760.14276526457728678No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT54990.13831328222524666No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.13773477604390813No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA47960.12063111503042062No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC43060.10830641812364288No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42950.10802974125430705No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC42880.10785367415563879No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40670.10229498432625535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.028.0592171
GTATTGG13650.020.8717961
ATTGGAC13200.019.9015163
CGAACGA2800.018.516
GGACCCT22200.018.2500026
TGGACCC22400.018.0870535
TTGGACC24150.017.3892354
TATACCG2450.016.6122445
GTATCAA182200.016.124042
GACCCTC24600.016.0934967
ACGAACG3350.016.01492515
TATTGGA19000.015.6763152
CGTCTGT8750.015.43428634
CTGTGCG3500.015.3285729
CGGTATA2950.014.42372826
CGTATAC2201.8189894E-1114.2954543
ACGTTTA2201.8189894E-1114.29545426
ACCCTCG27350.014.2723958
TAATACT6250.014.2084
CCGTATA2252.7284841E-1113.97777752
Produced by FastQC (version 0.11.5)