##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3975757
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434122608600074	31.0	31.0	34.0	30.0	34.0
2	31.627541119842082	31.0	31.0	34.0	30.0	34.0
3	31.710213174497333	31.0	31.0	34.0	30.0	34.0
4	35.497141047604266	37.0	35.0	37.0	33.0	37.0
5	35.3294544913082	37.0	35.0	37.0	33.0	37.0
6	35.36082210255808	37.0	35.0	37.0	32.0	37.0
7	35.2422728552072	37.0	35.0	37.0	32.0	37.0
8	35.2731842011471	37.0	35.0	37.0	32.0	37.0
9	36.78259184351559	39.0	37.0	39.0	32.0	39.0
10	36.65661357069861	39.0	35.0	39.0	32.0	39.0
11	36.75685636722768	39.0	35.0	39.0	32.0	39.0
12	36.62114183537877	39.0	35.0	39.0	32.0	39.0
13	36.65985169616755	39.0	35.0	39.0	32.0	39.0
14	37.67590272745542	39.0	37.0	41.0	32.0	41.0
15	37.66868171269019	39.0	36.0	41.0	32.0	41.0
16	37.62690778133573	39.0	36.0	41.0	32.0	41.0
17	37.68503180652137	39.0	37.0	41.0	32.0	41.0
18	37.66464650631314	39.0	37.0	41.0	32.0	41.0
19	37.722276034475954	40.0	37.0	41.0	32.0	41.0
20	37.685314016928096	39.0	37.0	41.0	32.0	41.0
21	37.63257739343727	39.0	37.0	41.0	32.0	41.0
22	37.52773069380246	39.0	36.0	41.0	32.0	41.0
23	37.41672868839821	39.0	36.0	41.0	32.0	41.0
24	37.34223344132954	39.0	36.0	41.0	31.0	41.0
25	37.284567190600434	39.0	36.0	41.0	31.0	41.0
26	37.06154224214407	39.0	36.0	41.0	31.0	41.0
27	36.91151848566198	39.0	35.0	40.0	31.0	41.0
28	36.79023189797566	39.0	35.0	40.0	30.0	41.0
29	36.653023814081195	39.0	35.0	40.0	30.0	41.0
30	36.468469778208274	38.0	35.0	40.0	30.0	41.0
31	36.23907270992669	38.0	35.0	40.0	30.0	41.0
32	36.101971272389136	38.0	35.0	40.0	30.0	41.0
33	36.16798184597298	38.0	35.0	40.0	30.0	41.0
34	36.13880928839464	38.0	35.0	40.0	30.0	41.0
35	36.060462699304814	38.0	35.0	40.0	30.0	41.0
36	35.98059765725119	38.0	35.0	40.0	29.0	41.0
37	35.85561089372414	38.0	35.0	40.0	29.0	41.0
38	35.742303415425035	38.0	35.0	40.0	28.0	41.0
39	35.676005600945935	38.0	35.0	40.0	27.0	41.0
40	35.52668208846768	38.0	34.0	40.0	26.0	41.0
41	35.38534045214534	38.0	34.0	40.0	26.0	41.0
42	35.21244230972869	38.0	34.0	40.0	25.0	41.0
43	34.88566051697828	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	8.0
15	19.0
16	50.0
17	94.0
18	251.0
19	545.0
20	1145.0
21	2365.0
22	4430.0
23	7609.0
24	12426.0
25	19864.0
26	30173.0
27	44769.0
28	63402.0
29	85434.0
30	110729.0
31	136673.0
32	167110.0
33	205044.0
34	253193.0
35	310230.0
36	395671.0
37	544687.0
38	724136.0
39	855698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58813026047618	21.95871125926459	13.44493639827585	23.00822208198338
2	18.172388302403792	22.733859237372908	35.88808370330481	23.20566875691849
3	19.783729236972984	25.98591915954622	31.750330817502174	22.48002078597862
4	12.960525504954152	15.81427134505454	35.72773688130336	35.49746626868795
5	12.057854642524681	39.75932130660903	34.37981747878454	13.803006572081744
6	31.63530869718647	36.38172554308525	15.763287343768747	16.219678415959528
7	25.600634042774743	34.2342854455139	21.64352600020575	18.52155451150561
8	27.817972778517397	33.0584339032793	20.209509786438154	18.914083531765147
9	25.911065490169545	13.65674511797376	22.828507879128427	37.603681512728265
10	16.584011547989476	26.997273726739333	33.577731234579986	22.840983490691208
11	32.48998869900751	21.92759768768564	24.534522607895802	21.047891005411046
12	20.825493107350372	26.911327830146558	31.204522811630593	21.058656250872478
13	30.86853648248623	20.55203575067591	28.055864581260874	20.523563185576986
14	21.37371574771798	22.13568384586885	27.89868696703546	28.59191343937771
15	23.553325819460294	28.705929461986734	25.95601290521528	21.784731813337686
16	22.595495650262325	27.819884364160085	26.538543477380532	23.046076508197054
17	20.528568521667697	26.724470333574207	27.298222703248715	25.448738441509377
18	21.426083133350453	25.81868056825404	31.194788816318503	21.560447482077
19	20.824034265675696	24.67117582890504	32.22548561192246	22.27930429349681
20	24.464473055068506	23.822507260881387	31.012433606983524	20.700586077066582
21	24.075339614569	24.774904502463304	30.05450282801489	21.095253054952806
22	22.74615878183702	24.28161479688019	30.958657684561707	22.01356873672108
23	21.778921599081634	25.64817215941518	31.826140279700194	20.746765961803
24	20.62814704218593	25.305771957390753	30.448390080178438	23.617690920244875
25	21.791397210644416	25.826779654792787	31.30840239984486	21.073420734717942
26	22.864475872142084	25.2627361279877	30.171486838858613	21.701301161011603
27	21.785536691503026	26.330432166754658	29.84440447441833	22.039626667323983
28	21.45259380792136	25.82089398320873	30.149428146639746	22.577084062230163
29	20.638962592532692	25.90787112995085	30.895424443697138	22.55774183381932
30	20.17603188524852	26.283070117212898	32.27033241719753	21.270565580341053
31	21.421002339931743	25.725591377943875	31.323644780100995	21.52976150202339
32	22.243487215139155	25.66909899171403	29.669091949030086	22.418321844116733
33	22.824885927384393	25.641632524321782	29.459899083369535	22.07358246492429
34	21.712267626014366	27.86266866913647	29.385523310403528	21.039540394445634
35	20.649576923338124	28.669659639661077	29.77249364083368	20.90826979616712
36	21.23316389809538	26.025861238501246	31.124160757309866	21.616814106093507
37	21.48164487920162	26.0318978247413	30.4623999907439	22.02405730531318
38	21.425580084497117	25.65727734366059	31.087060904376195	21.830081667466096
39	22.381750192479068	25.230314629390076	30.92123084987337	21.466704328257485
40	20.89247406217231	25.8246416971661	30.41991751507952	22.86296672558207
41	21.464013016892128	25.50842518795792	30.45963322205054	22.56792857309941
42	19.789941890311706	26.904737890167834	30.755853539338545	22.54946668018191
43	20.10950367439459	26.46587807051588	30.454250599319828	22.970367655769707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	479.0
2	660.0
3	1820.5
4	2981.0
5	2981.0
6	4964.0
7	6947.0
8	7339.0
9	7731.0
10	11547.0
11	15363.0
12	15363.0
13	27264.0
14	39165.0
15	63891.0
16	88617.0
17	86697.0
18	84777.0
19	84777.0
20	89828.0
21	94879.0
22	77206.5
23	59534.0
24	65860.5
25	72187.0
26	72187.0
27	78942.0
28	85697.0
29	91727.5
30	97758.0
31	105642.5
32	113527.0
33	113527.0
34	125794.0
35	138061.0
36	152295.0
37	166529.0
38	177552.5
39	188576.0
40	188576.0
41	200686.5
42	212797.0
43	238718.5
44	264640.0
45	319722.5
46	374805.0
47	374805.0
48	379897.5
49	384990.0
50	355839.0
51	326688.0
52	310977.5
53	295267.0
54	295267.0
55	261064.5
56	226862.0
57	194671.0
58	162480.0
59	145634.0
60	128788.0
61	128788.0
62	115015.5
63	101243.0
64	89174.0
65	77105.0
66	66225.5
67	55346.0
68	55346.0
69	46998.5
70	38651.0
71	32933.0
72	27215.0
73	21092.0
74	14969.0
75	14969.0
76	11565.5
77	8162.0
78	6557.0
79	4952.0
80	4010.5
81	3069.0
82	3069.0
83	2365.5
84	1662.0
85	1390.5
86	1119.0
87	998.0
88	877.0
89	877.0
90	733.5
91	590.0
92	360.5
93	131.0
94	88.5
95	46.0
96	46.0
97	29.5
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3975757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.550980841249704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67189963526192	26.913501300596927
2	13.956940091684892	10.481935799706585
3	5.543172793014316	6.244547260506516
4	2.6933098809513134	4.045457109566052
5	1.5260746427599794	2.8652799836298493
6	0.9325368595568371	2.101060424818677
7	0.6309379404064587	1.6584636958554337
8	0.4372596450304993	1.313562284255354
9	0.33312999081632555	1.1258422112510578
>10	1.9169450525757141	13.773607892164632
>50	0.2030520895065467	5.292503403727476
>100	0.1367379831341445	9.871985792665814
>500	0.010440630734982644	2.70958394327741
>1k	0.006358076405066374	4.718719764022385
>5k	6.023440804799724E-4	1.6206737435889174
>10k+	6.023440804799724E-4	5.263275390366992
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44079	1.108694520313993	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43121	1.0845984802391093	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39354	0.9898492287129218	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	22363	0.5624840753597365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14594	0.3670747482806419	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12935	0.3253468458962658	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11139	0.28017305886652527	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10927	0.2748407410211439	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10915	0.2745389117091412	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8964	0.2254664960660322	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8802	0.22139180035399547	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8135	0.2046151210951776	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7576	0.1905549056443842	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7553	0.18997639946304568	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6806	0.17118752479087632	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5676	0.14276526457728678	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5499	0.13831328222524666	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5476	0.13773477604390813	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4796	0.12063111503042062	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4306	0.10830641812364288	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4295	0.10802974125430705	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4288	0.10785367415563879	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4067	0.10229498432625535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5152442666893374E-5	0.0	0.0	0.0	0.0
6	7.545732800068012E-5	0.0	0.0	0.0	0.0
7	7.545732800068012E-5	0.0	0.0	0.0	0.0
8	1.006097706675735E-4	0.0	0.0	0.0	0.0
9	1.006097706675735E-4	0.0	0.0	2.5152442666893374E-5	0.0
10	1.006097706675735E-4	2.5152442666893374E-5	0.0	2.5152442666893374E-5	0.0
11	1.006097706675735E-4	2.5152442666893374E-5	0.0	1.2576221333446687E-4	0.0
12	1.006097706675735E-4	2.5152442666893374E-5	0.0	3.0182931200272047E-4	0.0
13	1.006097706675735E-4	2.5152442666893374E-5	0.0	3.5213419733650726E-4	0.0
14	1.006097706675735E-4	2.5152442666893374E-5	0.0	4.527439680040807E-4	0.0
15	1.006097706675735E-4	2.5152442666893374E-5	0.0	8.803354933412681E-4	0.0
16	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.0014839941173467092	0.0
17	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.0019367380853507897	0.0
18	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.00216311006935283	0.0
19	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.002565549152023124	0.0
20	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.003370427317363712	0.0
21	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.004502287237373914	0.0
22	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.007696647456069372	0.0
23	1.006097706675735E-4	2.5152442666893374E-5	0.0	0.013506861712121742	0.0
24	1.006097706675735E-4	5.030488533378675E-5	0.0	0.021807167792196554	0.0
25	1.006097706675735E-4	5.030488533378675E-5	0.0	0.02676219899757455	0.0
26	1.006097706675735E-4	5.030488533378675E-5	0.0	0.03463491355231218	0.0
27	1.006097706675735E-4	5.030488533378675E-5	0.0	0.09474925152618734	0.0
28	1.006097706675735E-4	5.030488533378675E-5	0.0	0.15702669956941534	0.0
29	1.006097706675735E-4	5.030488533378675E-5	0.0	0.23167914940475487	0.0
30	1.006097706675735E-4	5.030488533378675E-5	0.0	0.36868450461132307	0.0
31	1.006097706675735E-4	5.030488533378675E-5	0.0	0.5704825521278086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10470	0.0	28.059217	1
GTATTGG	1365	0.0	20.871796	1
ATTGGAC	1320	0.0	19.901516	3
CGAACGA	280	0.0	18.5	16
GGACCCT	2220	0.0	18.250002	6
TGGACCC	2240	0.0	18.087053	5
TTGGACC	2415	0.0	17.389235	4
TATACCG	245	0.0	16.612244	5
GTATCAA	18220	0.0	16.12404	2
GACCCTC	2460	0.0	16.093496	7
ACGAACG	335	0.0	16.014925	15
TATTGGA	1900	0.0	15.676315	2
CGTCTGT	875	0.0	15.434286	34
CTGTGCG	350	0.0	15.328572	9
CGGTATA	295	0.0	14.423728	26
CGTATAC	220	1.8189894E-11	14.295454	3
ACGTTTA	220	1.8189894E-11	14.295454	26
ACCCTCG	2735	0.0	14.272395	8
TAATACT	625	0.0	14.208	4
CCGTATA	225	2.7284841E-11	13.9777775	2
>>END_MODULE