FastQCFastQC Report
Wed 25 May 2016
SRR2088371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370434
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT364161.0804543272468767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360681.0701292474500317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT315630.9364669357121368No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG152800.45335407843618947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125520.3724149471551735No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88220.2617467068039309No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT83190.2468228127297553No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT78870.23400547229229232No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78110.23175056980792386No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA68190.20231815843300893No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62840.18644483173383605No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59360.1761197519369909No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59340.17606041239792858No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50910.15104879668315713No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47940.14223687513240135No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47040.13956659587459655No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44760.13280188842149113No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43240.12829208345275417No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37360.11084625896842959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.028.2915171
GTATTGG7950.019.547171
ATTGGAC7650.019.3464053
CGAACGA1601.8189894E-1218.516
ATAACGC2500.018.53
GTATCAA142500.016.6564922
TCTATAC3050.015.7704933
TTGGACC15100.015.682124
GGACCCT15200.015.3355266
GACCCTC14900.014.6510077
TGGACCC15750.014.565085
TCTAACG1406.004284E-714.5357152
ACACGCT4850.014.4948459
CGCACTA1152.2122393E-514.47826129
CGCATCG2059.276846E-1114.43902413
CCGTACG908.2798646E-414.3888882
ACGTTTA2450.014.34693826
AATGCGT3500.014.27142935
AGCCGTC2405.456968E-1213.87500112
TCTATCG800.00630195213.87529
Produced by FastQC (version 0.11.5)