##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3370434
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3140085223446	31.0	31.0	34.0	30.0	34.0
2	31.513387890105548	31.0	31.0	34.0	30.0	34.0
3	31.597666947342688	31.0	31.0	34.0	30.0	34.0
4	35.39671923556432	37.0	35.0	37.0	33.0	37.0
5	35.216116974846564	37.0	35.0	37.0	33.0	37.0
6	35.247889144246706	37.0	35.0	37.0	32.0	37.0
7	35.13248175160825	37.0	35.0	37.0	32.0	37.0
8	35.16750839802827	37.0	35.0	37.0	32.0	37.0
9	36.6591465668813	39.0	35.0	39.0	32.0	39.0
10	36.51494851998289	38.0	35.0	39.0	32.0	39.0
11	36.6227328587357	39.0	35.0	39.0	32.0	39.0
12	36.490118186559954	38.0	35.0	39.0	32.0	39.0
13	36.534096499145214	38.0	35.0	39.0	32.0	39.0
14	37.5341039165876	39.0	36.0	41.0	32.0	41.0
15	37.52319226544712	39.0	36.0	41.0	32.0	41.0
16	37.479677988057325	39.0	36.0	41.0	32.0	41.0
17	37.53481984812638	39.0	36.0	41.0	32.0	41.0
18	37.505247692136976	39.0	36.0	41.0	32.0	41.0
19	37.57168513016425	39.0	36.0	41.0	32.0	41.0
20	37.53908636098497	39.0	36.0	41.0	32.0	41.0
21	37.489226906683236	39.0	36.0	41.0	32.0	41.0
22	37.37940514485672	39.0	36.0	41.0	31.0	41.0
23	37.2789934471347	39.0	36.0	41.0	31.0	41.0
24	37.206697713113506	39.0	36.0	41.0	31.0	41.0
25	37.14331537125486	39.0	36.0	41.0	31.0	41.0
26	36.91659056370782	39.0	36.0	40.0	31.0	41.0
27	36.75630200739727	39.0	35.0	40.0	30.0	41.0
28	36.641818234684315	39.0	35.0	40.0	30.0	41.0
29	36.49426750382889	38.0	35.0	40.0	30.0	41.0
30	36.31544898965534	38.0	35.0	40.0	30.0	41.0
31	36.07884622573829	38.0	35.0	40.0	30.0	41.0
32	35.947246259680504	38.0	35.0	40.0	29.0	41.0
33	36.018503255070414	38.0	35.0	40.0	30.0	41.0
34	35.99460277222459	38.0	35.0	40.0	30.0	41.0
35	35.92564607406643	38.0	35.0	40.0	29.0	41.0
36	35.84479268841935	38.0	35.0	40.0	29.0	41.0
37	35.723612152025524	38.0	35.0	40.0	28.0	41.0
38	35.60773508693539	38.0	34.0	40.0	27.0	41.0
39	35.5439507196996	38.0	34.0	40.0	27.0	41.0
40	35.4014984420404	38.0	34.0	40.0	26.0	41.0
41	35.26575924643532	38.0	34.0	40.0	25.0	41.0
42	35.11508369545287	38.0	34.0	40.0	25.0	41.0
43	34.774700528181235	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	3.0
15	21.0
16	34.0
17	82.0
18	197.0
19	501.0
20	1078.0
21	2238.0
22	4029.0
23	7067.0
24	11445.0
25	18457.0
26	28071.0
27	40525.0
28	56637.0
29	76949.0
30	99145.0
31	121260.0
32	148984.0
33	180903.0
34	221343.0
35	269763.0
36	339788.0
37	459281.0
38	612693.0
39	669933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.845748648393645	22.45268710201713	13.764696178593026	22.936868070996198
2	18.102624172435952	23.37420047388556	35.45133950108502	23.071835852593463
3	19.82756523343878	26.010359496729503	32.05607942478624	22.105995845045477
4	13.392755947750349	16.311638204456756	35.86324491148618	34.432360936306715
5	12.092804665511919	39.871927472841776	34.4393926716856	13.595875189960699
6	31.41708159839356	36.944797020205705	15.723909739813923	15.914211641586812
7	25.03348233491592	34.70639092769655	21.998680288651254	18.261446448736276
8	27.41623185619419	33.54449308308663	20.509139179108686	18.530135881610498
9	25.750689673792753	13.72947816215953	23.007600801558496	37.512231362489224
10	16.2111466950547	27.64261219771697	34.04715831848361	22.099082788744713
11	32.24664835448491	22.28965171844338	24.613447407663227	20.85025251940848
12	20.34565874899197	27.26135565924151	31.688916026838086	20.704069564928435
13	30.419435597908162	21.050464124204776	28.16548254616468	20.364617731722383
14	21.057288171196944	22.402545191509464	28.36376561594145	28.17640102135215
15	23.181109613776744	29.10310660288853	26.168469698561076	21.547314084773653
16	22.137950186830537	28.28428030336746	26.803284087449864	22.774485422352136
17	20.171971918156533	27.239578048405637	27.974171872227732	24.6142781612101
18	20.986169733630746	26.466977249814118	31.3996951134483	21.147157903106837
19	20.56907211356164	25.38720532726646	32.45098405724604	21.592738501925865
20	23.829275398954554	24.593806020233597	31.284843435593164	20.29207514521869
21	23.45122319558846	25.349732408348597	30.427921151994074	20.771123244068864
22	22.273363014970773	25.11780381992349	31.101662278507753	21.507170886597986
23	21.434746979172413	26.37066917791596	31.769083744111292	20.425500098800335
24	20.384021760995765	25.94431458975313	30.738533969215837	22.93312968003527
25	21.40510687941078	26.4886658513414	31.37610764667102	20.730119622576794
26	22.297899914373044	25.917967834409456	30.34487546707635	21.439256784141154
27	21.308116402813408	26.97391493202359	30.19887053121349	21.519098133949516
28	21.129356041388142	26.619865572208205	30.265360484732827	21.98541790167082
29	20.3306458456092	26.466354184653962	31.178685000210653	22.02431496952618
30	20.056971891453742	26.875292618102005	32.22395098079357	20.843784509650686
31	21.06384519026333	26.40149606845884	31.473483830272304	21.061174911005526
32	21.753815680710556	26.448047936853236	30.088855025791933	21.70928135664428
33	22.329320200306547	26.44012610838842	29.795717702824025	21.43483598848101
34	21.2944979785986	28.182305305488846	29.784947576484218	20.738249139428337
35	20.32708547326546	28.790624590186308	30.11075131570593	20.7715386208423
36	20.849095398396763	26.46190371922429	31.347891695846887	21.341109186532062
37	21.083071200919527	26.2871191069162	30.86762713644593	21.762182555718343
38	21.013228563443164	26.079312041119927	31.38797555448349	21.51948384095342
39	21.747466350030887	25.588366364687754	31.423786966307603	21.24038031897376
40	20.452291900686976	25.999144323846725	30.988353428668237	22.56021034679807
41	20.954719777927707	25.716065052749883	30.972836139203437	22.35637903011897
42	19.31887703482697	26.94863628838304	31.24852763768702	22.483959039102977
43	19.532796073146663	26.6642218776573	31.049087446898533	22.753894602297507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	355.0
1	492.0
2	629.0
3	1644.0
4	2659.0
5	2659.0
6	4464.5
7	6270.0
8	6526.5
9	6783.0
10	10232.5
11	13682.0
12	13682.0
13	24041.5
14	34401.0
15	55380.5
16	76360.0
17	74510.0
18	72660.0
19	72660.0
20	79227.0
21	85794.0
22	72132.0
23	58470.0
24	64764.5
25	71059.0
26	71059.0
27	77901.5
28	84744.0
29	89171.0
30	93598.0
31	100222.0
32	106846.0
33	106846.0
34	117352.0
35	127858.0
36	139862.5
37	151867.0
38	159674.0
39	167481.0
40	167481.0
41	176610.5
42	185740.0
43	205188.0
44	224636.0
45	265500.5
46	306365.0
47	306365.0
48	309537.0
49	312709.0
50	287484.0
51	262259.0
52	247992.5
53	233726.0
54	233726.0
55	208276.5
56	182827.0
57	159837.5
58	136848.0
59	121331.0
60	105814.0
61	105814.0
62	93867.5
63	81921.0
64	71468.5
65	61016.0
66	51838.5
67	42661.0
68	42661.0
69	35617.0
70	28573.0
71	23993.0
72	19413.0
73	15122.0
74	10831.0
75	10831.0
76	8234.0
77	5637.0
78	4471.5
79	3306.0
80	2683.5
81	2061.0
82	2061.0
83	1551.0
84	1041.0
85	865.0
86	689.0
87	564.0
88	439.0
89	439.0
90	375.0
91	311.0
92	180.0
93	49.0
94	40.5
95	32.0
96	32.0
97	20.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3370434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.35328587021779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96044554572786	29.038404304549974
2	13.812006604859636	11.147197019344745
3	5.566363376795891	6.738631578040663
4	2.718792458820463	4.388488372502979
5	1.5490451858393266	3.12545316050295
6	0.933510479610144	2.260212914793135
7	0.6322995002074241	1.7860753741226212
8	0.4458094107003918	1.4391899674900992
9	0.32025532640732657	1.1631019264177285
>10	1.772801007254672	13.207508779975615
>50	0.1571379437221921	4.419576375963347
>100	0.11725933416660712	9.149097881219689
>500	0.008019830009304191	2.2380701873216005
>1k	0.005223925969155231	4.115816418650576
>5k	6.621877989070012E-4	1.8676217695520054
>10k+	3.678821105038895E-4	3.915553969552309
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36416	1.0804543272468767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36068	1.0701292474500317	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31563	0.9364669357121368	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15280	0.45335407843618947	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12552	0.3724149471551735	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8822	0.2617467068039309	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8319	0.2468228127297553	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7887	0.23400547229229232	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7811	0.23175056980792386	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6819	0.20231815843300893	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6284	0.18644483173383605	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5936	0.1761197519369909	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5934	0.17606041239792858	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5091	0.15104879668315713	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4794	0.14223687513240135	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4704	0.13956659587459655	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4476	0.13280188842149113	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4324	0.12829208345275417	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3736	0.11084625896842959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.90093085934927E-5	0.0	0.0	0.0	0.0
7	8.90093085934927E-5	0.0	0.0	0.0	0.0
8	8.90093085934927E-5	0.0	0.0	2.9669769531164234E-5	0.0
9	8.90093085934927E-5	0.0	0.0	2.9669769531164234E-5	0.0
10	8.90093085934927E-5	0.0	0.0	2.9669769531164234E-5	0.0
11	8.90093085934927E-5	0.0	0.0	1.1867907812465694E-4	0.0
12	8.90093085934927E-5	0.0	0.0	2.0768838671814964E-4	0.0
13	1.1867907812465694E-4	0.0	0.0	3.560372343739708E-4	0.0
14	1.1867907812465694E-4	0.0	0.0	4.450465429674635E-4	0.0
15	1.1867907812465694E-4	0.0	0.0	9.494326249972555E-4	0.0
16	1.1867907812465694E-4	0.0	0.0	0.001394479167964719	0.0
17	1.1867907812465694E-4	0.0	0.0	0.0021955629453061535	0.0
18	1.1867907812465694E-4	0.0	0.0	0.002373581562493139	0.0
19	1.1867907812465694E-4	0.0	0.0	0.002877967644522931	0.0
20	1.1867907812465694E-4	0.0	0.0	0.003768060730457858	0.0
21	1.1867907812465694E-4	0.0	0.0	0.004954851511704428	0.0
22	1.1867907812465694E-4	0.0	0.0	0.00792182846482085	0.0
23	1.1867907812465694E-4	0.0	0.0	0.012579982281213636	0.0
24	1.1867907812465694E-4	0.0	0.0	0.02121388521478243	0.0
25	1.1867907812465694E-4	0.0	0.0	0.0264654344217985	0.0
26	1.1867907812465694E-4	0.0	0.0	0.035485044359272425	0.0
27	1.1867907812465694E-4	0.0	0.0	0.09954207677705601	0.0
28	1.1867907812465694E-4	0.0	0.0	0.16665509545654952	0.0
29	1.1867907812465694E-4	0.0	0.0	0.2434107892336714	0.0
30	1.1867907812465694E-4	0.0	0.0	0.3817609245574902	0.0
31	1.1867907812465694E-4	0.0	0.0	0.6012875493185744	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8370	0.0	28.291517	1
GTATTGG	795	0.0	19.54717	1
ATTGGAC	765	0.0	19.346405	3
CGAACGA	160	1.8189894E-12	18.5	16
ATAACGC	250	0.0	18.5	3
GTATCAA	14250	0.0	16.656492	2
TCTATAC	305	0.0	15.770493	3
TTGGACC	1510	0.0	15.68212	4
GGACCCT	1520	0.0	15.335526	6
GACCCTC	1490	0.0	14.651007	7
TGGACCC	1575	0.0	14.56508	5
TCTAACG	140	6.004284E-7	14.535715	2
ACACGCT	485	0.0	14.494845	9
CGCACTA	115	2.2122393E-5	14.478261	29
CGCATCG	205	9.276846E-11	14.439024	13
CCGTACG	90	8.2798646E-4	14.388888	2
ACGTTTA	245	0.0	14.346938	26
AATGCGT	350	0.0	14.271429	35
AGCCGTC	240	5.456968E-12	13.875001	12
TCTATCG	80	0.006301952	13.875	29
>>END_MODULE