FastQCFastQC Report
Wed 25 May 2016
SRR2088370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3495136
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT408961.1700832242293289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT395691.1321161751645716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT354281.0136372375781657No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG179110.5124550232093973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131720.3768665940323925No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102140.29223469415782394No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98540.28193466577552345No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT92160.263680726586891No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81330.23269480787013724No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA78570.2247981194437069No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT73010.20889029783104293No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT70520.20176611153328508No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG68220.19518553784459317No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58340.16691768217316866No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA56870.16271183725039598No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT52130.1491501332137004No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA51960.14866374298453622No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.14677540444778114No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43730.12511673365499942No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38850.11115447295899215No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36950.1057183468683336No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35050.10028222077767503No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.10016777601787169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88300.029.4575311
GTATTGG8700.022.1149431
ATTGGAC8350.020.604793
ACGTTTA1950.017.07692326
TTGGACC15850.016.924294
GGACCCT15450.016.8834976
ACGGACC2200.016.8181828
GTATCAA155950.016.6316132
TATACTG4850.015.6391755
TGGACCC16750.015.2417915
TATACCG2800.015.1964285
CGACGAG2450.015.10204124
TATTGGA12650.015.0632412
ACCGTCC750.00410663514.88
GACCCTC17200.014.5203497
TTCGCAC2101.364242E-1014.09523831
AGTGCGC2009.858923E-1013.8749998
TGTACCG1203.30415E-513.8749995
CGTCTGT8350.013.5149734
ATCTGCG4500.013.15555636
Produced by FastQC (version 0.11.5)