##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3495136
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415045079790886	31.0	31.0	34.0	30.0	34.0
2	31.61247115992053	31.0	31.0	34.0	30.0	34.0
3	31.690068998745687	31.0	31.0	34.0	30.0	34.0
4	35.47890468353735	37.0	35.0	37.0	33.0	37.0
5	35.30423050776851	37.0	35.0	37.0	33.0	37.0
6	35.33818598189026	37.0	35.0	37.0	32.0	37.0
7	35.22097738113767	37.0	35.0	37.0	32.0	37.0
8	35.25474230473435	37.0	35.0	37.0	32.0	37.0
9	36.7534951429644	39.0	37.0	39.0	32.0	39.0
10	36.62678648270053	39.0	35.0	39.0	32.0	39.0
11	36.73514421184183	39.0	35.0	39.0	32.0	39.0
12	36.60721185098377	39.0	35.0	39.0	32.0	39.0
13	36.6497901655329	39.0	35.0	39.0	32.0	39.0
14	37.66241199197971	39.0	37.0	41.0	32.0	41.0
15	37.65382405720407	39.0	36.0	41.0	32.0	41.0
16	37.60284235005447	39.0	36.0	41.0	32.0	41.0
17	37.668635211905915	39.0	36.0	41.0	32.0	41.0
18	37.65688488230501	39.0	37.0	41.0	32.0	41.0
19	37.720208312351794	40.0	37.0	41.0	32.0	41.0
20	37.68649803612792	39.0	37.0	41.0	32.0	41.0
21	37.63838860633749	39.0	37.0	41.0	32.0	41.0
22	37.53416290524889	39.0	36.0	41.0	32.0	41.0
23	37.44433063548886	39.0	36.0	41.0	32.0	41.0
24	37.3768010744074	39.0	36.0	41.0	31.0	41.0
25	37.31588727877828	39.0	36.0	41.0	31.0	41.0
26	37.099017892293745	39.0	36.0	41.0	31.0	41.0
27	36.93887076211055	39.0	35.0	40.0	31.0	41.0
28	36.82713176254086	39.0	35.0	40.0	30.0	41.0
29	36.684846884355856	39.0	35.0	40.0	30.0	41.0
30	36.50532282577846	38.0	35.0	40.0	30.0	41.0
31	36.26923501689205	38.0	35.0	40.0	30.0	41.0
32	36.133354181353745	38.0	35.0	40.0	30.0	41.0
33	36.19274042555139	38.0	35.0	40.0	30.0	41.0
34	36.16389405161917	38.0	35.0	40.0	30.0	41.0
35	36.08800258416267	38.0	35.0	40.0	30.0	41.0
36	36.00511768354651	38.0	35.0	40.0	29.0	41.0
37	35.87623457284638	38.0	35.0	40.0	29.0	41.0
38	35.75836390915833	38.0	35.0	40.0	28.0	41.0
39	35.6932602908728	38.0	35.0	40.0	27.0	41.0
40	35.53813499674977	38.0	35.0	40.0	26.0	41.0
41	35.40678274035688	38.0	34.0	40.0	26.0	41.0
42	35.23779789970977	38.0	34.0	40.0	25.0	41.0
43	34.89882396564826	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	4.0
15	13.0
16	29.0
17	64.0
18	202.0
19	444.0
20	977.0
21	2016.0
22	3778.0
23	6440.0
24	10809.0
25	17285.0
26	26899.0
27	39022.0
28	55018.0
29	75710.0
30	97503.0
31	119977.0
32	148820.0
33	181078.0
34	223092.0
35	273464.0
36	346850.0
37	473382.0
38	624266.0
39	767990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.893001016269466	22.51446009540115	13.766502934363642	22.82603595396574
2	17.69550598317204	23.183675828351173	36.150352947639234	22.970465240837555
3	19.434150774104356	26.173974346062646	32.43556187799273	21.95631300184027
4	12.880471603966198	16.25092700255441	36.24268125761058	34.62592013586882
5	11.709243932138836	40.08195961473316	34.74837602885839	13.460420424269614
6	30.937022193127824	37.09972945258782	15.893201294599123	16.07004705968523
7	24.746476245845656	34.9731169259222	22.121342345476684	18.159064482755465
8	27.361682063301686	33.59651813262774	20.577596980489457	18.464202823581115
9	25.59866053853126	13.815027512520256	23.41811591880831	37.16819603014017
10	16.16938511119453	27.87001707515816	34.10957399082611	21.8510238228212
11	31.776417283905406	22.413634262014412	24.92933036082144	20.880618093258747
12	20.240585774058577	27.671169304999864	31.6939598344671	20.39428508647446
13	30.384625948747058	21.292418950221105	28.454944242513026	19.86801085851881
14	20.916296247127438	22.62484206623147	28.64847033134047	27.810391355300624
15	22.977331926425755	29.32947387455023	26.42643948618881	21.26675471283521
16	21.873798330022066	28.438664475430997	27.2181683345083	22.469368860038635
17	19.736885774974137	27.540130055025042	28.098362982155773	24.62462118784505
18	20.64320243904672	26.714039167574594	31.875125889235782	20.7676325041429
19	20.129746024189043	25.52564478177673	33.08995701454822	21.254652179486005
20	23.67904997115992	24.69929639361673	31.777762009833094	19.843891625390256
21	23.1246509434826	25.486447451544088	30.912559625719858	20.476341979253455
22	21.913882607143183	25.230606191003723	31.688437874806592	21.167073327046502
23	21.206098990139438	26.54008313267352	32.392273147597116	19.861544729589923
24	19.860600560321544	26.051947620922334	31.214751013980575	22.87270080477555
25	21.02327348635361	26.64806176354797	32.08341535207786	20.24524939802056
26	21.908360647482674	26.08264742773958	30.896823471246897	21.11216845353085
27	20.83303768437051	27.226951969823205	30.602042381183452	21.337967964622838
28	20.68806478488963	26.82808337071862	30.77213590544116	21.711715938950586
29	19.76818641678035	26.71049138002069	31.74242719024381	21.778895012955147
30	19.499184008862603	27.12924475614111	32.953023859443526	20.418547375552766
31	20.709122620693442	26.55230011078253	31.980014511595545	20.758562756928487
32	21.52153735934739	26.692752442251173	30.459272543328787	21.32643765507265
33	22.056022998818932	26.6902346575355	30.12280494950697	21.130937394138595
34	20.911518178405647	28.654450070040195	30.135250817135585	20.298780934418577
35	19.809071807220093	29.352076714611393	30.469858683610596	20.368992794557922
36	20.528814901623285	26.714067778764544	31.818304065993424	20.938813253618743
37	20.676105307490182	26.680764353661772	31.289225941422593	21.35390439742545
38	20.65933915017899	26.444807870137243	31.8172740631552	21.07857891652857
39	21.49864840738672	26.013236680918855	31.73925134815927	20.748863563535153
40	20.08093533413292	26.453190948792837	31.237067742142223	22.22880597493202
41	20.79366868699816	25.99629885646796	31.247339159334576	21.962693297199305
42	18.8203549160891	27.44511229319832	31.538858573743628	22.195674216968953
43	19.21352988839347	27.02621586112815	31.333544674656437	22.426709575821942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	457.5
2	585.0
3	1648.0
4	2711.0
5	2711.0
6	4488.5
7	6266.0
8	6749.5
9	7233.0
10	10621.0
11	14009.0
12	14009.0
13	25001.0
14	35993.0
15	59347.0
16	82701.0
17	81178.5
18	79656.0
19	79656.0
20	87173.0
21	94690.0
22	79096.0
23	63502.0
24	70545.5
25	77589.0
26	77589.0
27	84029.5
28	90470.0
29	96332.5
30	102195.0
31	111081.5
32	119968.0
33	119968.0
34	130324.5
35	140681.0
36	153630.0
37	166579.0
38	172292.0
39	178005.0
40	178005.0
41	187068.5
42	196132.0
43	214847.0
44	233562.0
45	278682.0
46	323802.0
47	323802.0
48	325890.0
49	327978.0
50	298818.0
51	269658.0
52	253693.0
53	237728.0
54	237728.0
55	210870.0
56	184012.0
57	155789.5
58	127567.0
59	112036.5
60	96506.0
61	96506.0
62	85854.5
63	75203.0
64	65184.5
65	55166.0
66	46594.5
67	38023.0
68	38023.0
69	31997.5
70	25972.0
71	21775.0
72	17578.0
73	13838.5
74	10099.0
75	10099.0
76	7767.0
77	5435.0
78	4337.0
79	3239.0
80	2618.5
81	1998.0
82	1998.0
83	1490.5
84	983.0
85	807.0
86	631.0
87	496.5
88	362.0
89	362.0
90	306.0
91	250.0
92	157.5
93	65.0
94	40.0
95	15.0
96	15.0
97	11.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3495136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.79703956913044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74541211744744	27.44712553255048
2	14.39463987321491	11.169388254890034
3	5.876873019172414	6.840158252027615
4	2.8441937011823257	4.41385182268169
5	1.5862241923521117	3.0770401378098424
6	0.975338113761675	2.270413883573565
7	0.6507831490088647	1.7673921708114164
8	0.4481249696141819	1.3908737742429436
9	0.3429488268282422	1.1974859284177959
>10	1.8254230071267457	13.236125455269509
>50	0.17553200247522233	4.740017528806632
>100	0.11768882062452711	8.705485657659075
>500	0.009441800528841719	2.4580808005614583
>1k	0.00604865346319039	4.404295149907085
>5k	8.851687994912765E-4	2.3837591424937985
>10k+	4.425843997456383E-4	4.4985065082970195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40896	1.1700832242293289	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39569	1.1321161751645716	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35428	1.0136372375781657	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17911	0.5124550232093973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13172	0.3768665940323925	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10214	0.29223469415782394	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9854	0.28193466577552345	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9216	0.263680726586891	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8133	0.23269480787013724	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7857	0.2247981194437069	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7301	0.20889029783104293	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7052	0.20176611153328508	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6822	0.19518553784459317	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5834	0.16691768217316866	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5687	0.16271183725039598	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5213	0.1491501332137004	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5196	0.14866374298453622	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5130	0.14677540444778114	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4373	0.12511673365499942	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3885	0.11115447295899215	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3695	0.1057183468683336	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3505	0.10028222077767503	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3501	0.10016777601787169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.8611189950834532E-5	0.0
6	0.0	0.0	0.0	2.8611189950834532E-5	0.0
7	0.0	0.0	0.0	2.8611189950834532E-5	0.0
8	0.0	0.0	0.0	2.8611189950834532E-5	0.0
9	0.0	0.0	0.0	2.8611189950834532E-5	0.0
10	0.0	0.0	0.0	5.7222379901669065E-5	0.0
11	0.0	0.0	0.0	2.8611189950834533E-4	0.0
12	0.0	0.0	0.0	4.005566593116834E-4	0.0
13	0.0	0.0	0.0	5.150014191150215E-4	0.0
14	0.0	0.0	0.0	5.722237990166907E-4	0.0
15	0.0	0.0	0.0	8.297245085742014E-4	0.0
16	0.0	0.0	0.0	0.001344725927689223	0.0
17	0.0	0.0	0.0	0.0017166713970500718	0.0
18	0.0	0.0	0.0	0.0019455609166567482	0.0
19	0.0	0.0	0.0	0.002260284006115928	0.0
20	0.0	0.0	0.0	0.0027180630453292804	0.0
21	0.0	0.0	0.0	0.003605009933805151	0.0
22	0.0	0.0	0.0	0.0056364044203144024	0.0
23	0.0	0.0	0.0	0.009899471722988747	0.0
24	0.0	0.0	0.0	0.016623101361434862	0.0
25	0.0	0.0	0.0	0.021572837222929235	0.0
26	0.0	0.0	0.0	0.030385083727786272	0.0
27	0.0	0.0	0.0	0.0938733142286881	0.0
28	0.0	0.0	0.0	0.15773349019895078	0.0
29	0.0	0.0	0.0	0.2340681449877773	0.0
30	0.0	0.0	0.0	0.36791129157778124	0.0
31	0.0	0.0	0.0	0.5752851963414299	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8830	0.0	29.457531	1
GTATTGG	870	0.0	22.114943	1
ATTGGAC	835	0.0	20.60479	3
ACGTTTA	195	0.0	17.076923	26
TTGGACC	1585	0.0	16.92429	4
GGACCCT	1545	0.0	16.883497	6
ACGGACC	220	0.0	16.818182	8
GTATCAA	15595	0.0	16.631613	2
TATACTG	485	0.0	15.639175	5
TGGACCC	1675	0.0	15.241791	5
TATACCG	280	0.0	15.196428	5
CGACGAG	245	0.0	15.102041	24
TATTGGA	1265	0.0	15.063241	2
ACCGTCC	75	0.004106635	14.8	8
GACCCTC	1720	0.0	14.520349	7
TTCGCAC	210	1.364242E-10	14.095238	31
AGTGCGC	200	9.858923E-10	13.874999	8
TGTACCG	120	3.30415E-5	13.874999	5
CGTCTGT	835	0.0	13.51497	34
ATCTGCG	450	0.0	13.155556	36
>>END_MODULE