FastQCFastQC Report
Wed 25 May 2016
SRR2088369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127801
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212581.884907000437134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201351.7853326961050753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168851.4971612899793492No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG94730.8399531477627701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.5821949084989284No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT59750.5297920466465272No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA56080.49725084478556053No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT55200.48944804978892553No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48620.4311044235640862No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA46330.4107994229478428No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT41350.3666426967168853No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37400.3316187873569894No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34660.30732372111746664No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31080.2755805323811559No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27860.24702939614346858No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27350.24250732177041873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.23771924302248357No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24890.2206949630298253No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG21680.19223249491709973No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA21650.19196649054221446No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC21300.18886310616855279No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19090.16926745055200343No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19000.16846943742734755No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.15401653305858037No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT17370.15401653305858037No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17120.1517998299345363No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.13690358494096033No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA13620.12076598619791966No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA13490.11961330057341678No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG13020.11544589870021396No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG12860.11402720870082578No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12810.11358386807601695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12620.11189917370174349No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC11670.10347570183037609No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.10312102933052905No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA11520.10214567995594967No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA11320.10037231745671443No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA11310.10028364933175268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.030.2968271
CGATTGC358.869293E-426.4285731
ATTGGAC4350.022.5402283
GTATTGG4400.022.284091
GTTCGCT705.1017832E-621.14285737
TAGTGCG450.00382576420.5555577
AGTGCGC656.9019836E-519.9230778
GCCGTCA656.9019836E-519.92307713
TGGACCC7200.019.5277795
GGACCCT7300.019.2602736
TATTGGA5200.019.2115382
TTGGACC8000.018.731254
GTATCAA79300.018.6166462
CTCTTAC500.00703480318.51
CTACGAT500.00703480318.51
TATAACG500.00703480318.52
GTGTAGA1156.40448E-817.6956521
ACGATCT953.6062647E-617.52631632
TGTTCGC953.6062647E-617.52631636
CGTCTGT3100.017.30645234
Produced by FastQC (version 0.11.5)