##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127801
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098105073501443	31.0	31.0	33.0	30.0	34.0
2	31.27844451281742	31.0	31.0	34.0	28.0	34.0
3	31.317523215531818	31.0	31.0	34.0	28.0	34.0
4	35.16281152437354	35.0	35.0	37.0	32.0	37.0
5	35.010378604026776	35.0	35.0	37.0	32.0	37.0
6	35.08293839072673	37.0	35.0	37.0	32.0	37.0
7	34.9369392295272	36.0	35.0	37.0	32.0	37.0
8	35.01500176006228	37.0	35.0	37.0	32.0	37.0
9	36.403376127526045	38.0	35.0	39.0	32.0	39.0
10	36.276628589618205	38.0	35.0	39.0	32.0	39.0
11	36.418021441725976	38.0	35.0	39.0	32.0	39.0
12	36.255076915165	38.0	35.0	39.0	32.0	39.0
13	36.30402881359389	38.0	35.0	39.0	32.0	39.0
14	37.16862194660228	39.0	36.0	41.0	31.0	41.0
15	37.161297072799194	39.0	36.0	41.0	31.0	41.0
16	37.07694531216057	39.0	36.0	40.0	31.0	41.0
17	37.23109307404409	39.0	36.0	40.0	32.0	41.0
18	37.23097869216289	39.0	36.0	40.0	31.0	41.0
19	37.325045819253575	39.0	36.0	41.0	31.0	41.0
20	37.30056898335788	39.0	36.0	40.0	31.0	41.0
21	37.24937644141121	39.0	36.0	41.0	31.0	41.0
22	37.13376561999856	39.0	36.0	40.0	31.0	41.0
23	37.00781432185288	39.0	36.0	40.0	31.0	41.0
24	36.93771418893936	39.0	35.0	40.0	31.0	41.0
25	36.86112000255364	39.0	35.0	40.0	31.0	41.0
26	36.59664869954894	38.0	35.0	40.0	30.0	41.0
27	36.40429650266315	38.0	35.0	40.0	30.0	41.0
28	36.25294799348467	38.0	35.0	40.0	30.0	41.0
29	36.07751899492907	38.0	35.0	40.0	30.0	41.0
30	35.84848213470284	38.0	34.0	40.0	29.0	41.0
31	35.53698480494342	38.0	34.0	40.0	28.0	41.0
32	35.392651717811916	37.0	34.0	40.0	27.0	41.0
33	35.381716277960386	38.0	34.0	40.0	27.0	41.0
34	35.286228687507815	38.0	34.0	40.0	27.0	41.0
35	35.15078457990372	38.0	34.0	40.0	26.0	41.0
36	35.028251437975314	38.0	34.0	40.0	25.0	41.0
37	34.85981037434796	38.0	33.0	40.0	25.0	41.0
38	34.70465002247737	38.0	33.0	40.0	24.0	41.0
39	34.55586756883528	38.0	33.0	40.0	24.0	41.0
40	34.31042799217238	38.0	33.0	40.0	23.0	41.0
41	34.11527831594404	38.0	33.0	40.0	21.0	41.0
42	33.84496555686686	37.0	33.0	40.0	20.0	41.0
43	33.47544114608871	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	4.0
16	15.0
17	51.0
18	100.0
19	258.0
20	473.0
21	1047.0
22	1851.0
23	3162.0
24	5149.0
25	8137.0
26	11968.0
27	16925.0
28	23677.0
29	30960.0
30	38929.0
31	46728.0
32	55213.0
33	67158.0
34	80130.0
35	95743.0
36	121553.0
37	163338.0
38	196611.0
39	158616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.951107509214836	23.69451702915674	14.729105578023075	20.625269883605352
2	18.689290043190244	23.894906991570323	35.302593276650754	22.11320968858868
3	21.069231185288896	26.149471404973042	32.84728422833461	19.934013181403458
4	13.19958042243268	16.30021608422053	34.09723878592057	36.40296470742622
5	10.91167679404434	41.56646429644946	33.71853722420888	13.803321685297318
6	31.22767225778307	35.824405192050726	14.839408725475506	18.108513824690704
7	23.747629236008834	36.61133480108636	20.712253314192843	18.928782648711962
8	28.7503735144764	32.81110763335021	19.87726558142793	18.56125327074546
9	25.299321422839665	13.267766210528276	24.33124283450715	37.1016695321249
10	16.98304931455106	28.035885763534523	32.585092582822675	22.39597233909174
11	31.050690680359388	21.398278597021992	25.348443564068486	22.202587158550134
12	20.47214003179639	28.22847293095147	31.4861398420466	19.813247195205538
13	32.072590820543695	20.39056535683157	29.653724371586833	17.883119451037903
14	20.955381312837993	22.61161321899874	29.33655848859861	27.09644697956466
15	23.37992252179241	28.602120409540337	27.83824451299476	20.1797125556725
16	21.387283749526734	27.951917049195735	28.075077074767623	22.585722126509907
17	18.900320180599238	26.840994111549822	28.388252892132567	25.870432815718374
18	20.11445281570064	25.161265152274204	34.164626560891506	20.55965547113365
19	19.290814602930837	23.669157945417677	35.78831726519129	21.251710186460198
20	24.59343448001908	22.149740956072925	34.428857573277554	18.82796699063044
21	24.16862549332728	23.311736733696815	32.92345014767676	19.596187625299144
22	21.774320114984825	22.931882486360625	34.07817513905378	21.215622259600764
23	20.59104398737011	25.447840532150618	35.28477098353344	18.676344496945827
24	18.855099436868738	24.241688028295773	33.45545889744733	23.447753637388153
25	20.289395026250197	25.43826437465475	34.76641712500698	19.50592347408807
26	22.102392177343344	24.144507763337682	33.12756417133873	20.625535887980238
27	20.617378420483757	25.949702119434193	32.53002967722142	20.90288978286063
28	20.139457226939857	25.449525226524894	32.750990644626135	21.660026901909113
29	19.03030765179318	25.324680506578733	33.83451513165887	21.810496709969225
30	18.267318436497217	25.72368706890666	36.14192574753879	19.867068747057324
31	19.91104813703836	24.884709270518467	34.60016439070368	20.604078201739494
32	21.34277235079593	25.3391334109475	32.160815604880646	21.157278633375924
33	22.595741624630588	25.170309301020303	31.434889665818705	20.799059408530404
34	20.48481957366592	28.718541657615127	31.55911370889013	19.237525059828815
35	18.705427641933284	29.788677257778634	31.916268916236106	19.589626184051973
36	19.834350208946436	25.45608666777206	34.495181330748956	20.21438179253255
37	19.99661287762646	26.03367083377298	33.211089545052715	20.758626743547843
38	20.457243786802813	25.38639352155212	33.63722855361895	20.519134138026125
39	21.91406107992456	24.927535974875	33.15469661757704	20.0037063276234
40	19.71420489962325	26.04040961127007	32.009547783695886	22.235837705410795
41	20.75259731105044	25.625442786449028	31.98436603620674	21.637593866293788
42	18.04378609346862	27.596712540598915	32.416800481645254	21.942700884287213
43	18.724225284425177	27.31155585072189	31.955814899969052	22.008403964883875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	104.0
2	152.0
3	460.5
4	769.0
5	769.0
6	1548.0
7	2327.0
8	2657.0
9	2987.0
10	4580.0
11	6173.0
12	6173.0
13	11408.5
14	16644.0
15	28358.0
16	40072.0
17	37790.0
18	35508.0
19	35508.0
20	37855.5
21	40203.0
22	30131.5
23	20060.0
24	21163.5
25	22267.0
26	22267.0
27	23323.0
28	24379.0
29	24736.0
30	25093.0
31	26362.0
32	27631.0
33	27631.0
34	30286.0
35	32941.0
36	36756.5
37	40572.0
38	42873.5
39	45175.0
40	45175.0
41	48233.5
42	51292.0
43	61571.5
44	71851.0
45	96633.5
46	121416.0
47	121416.0
48	123588.0
49	125760.0
50	112832.0
51	99904.0
52	93130.5
53	86357.0
54	86357.0
55	72954.5
56	59552.0
57	47881.0
58	36210.0
59	31286.0
60	26362.0
61	26362.0
62	23617.5
63	20873.0
64	18145.0
65	15417.0
66	13293.5
67	11170.0
68	11170.0
69	9277.0
70	7384.0
71	6207.0
72	5030.0
73	3960.0
74	2890.0
75	2890.0
76	2173.0
77	1456.0
78	1103.0
79	750.0
80	597.0
81	444.0
82	444.0
83	345.5
84	247.0
85	216.0
86	185.0
87	151.5
88	118.0
89	118.0
90	101.5
91	85.0
92	53.5
93	22.0
94	17.0
95	12.0
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1127801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.11682439068063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90635241014321	35.25214516978127
2	11.105041163393688	9.798383017134379
3	3.4361592315527085	4.547773001904808
4	1.531046029262173	2.701795552280326
5	0.8363602855735834	1.844877992299464
6	0.5626427581788188	1.4893207054357882
7	0.3934338130304059	1.2149935307172806
8	0.28533397763415713	1.0070423187184403
9	0.2320777383707084	0.9214679545818178
>10	1.4746105902779134	12.558787327203156
>50	0.1363206018345834	4.113785611783148
>100	0.0822716063213829	6.851598909795786
>500	0.008872428074434413	2.9028489489948788
>1k	0.007864197611430502	6.662470238027442
>5k	0.0010082304630039105	2.948307433654754
>10k+	6.049382778023464E-4	5.184402287687277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21258	1.884907000437134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20135	1.7853326961050753	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16885	1.4971612899793492	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9473	0.8399531477627701	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6566	0.5821949084989284	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5975	0.5297920466465272	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5608	0.49725084478556053	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5520	0.48944804978892553	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4862	0.4311044235640862	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4633	0.4107994229478428	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4135	0.3666426967168853	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3740	0.3316187873569894	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3466	0.30732372111746664	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3108	0.2755805323811559	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2786	0.24702939614346858	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2735	0.24250732177041873	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2681	0.23771924302248357	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2489	0.2206949630298253	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2168	0.19223249491709973	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2165	0.19196649054221446	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2130	0.18886310616855279	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1909	0.16926745055200343	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1900	0.16846943742734755	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1737	0.15401653305858037	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1737	0.15401653305858037	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1712	0.1517998299345363	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1544	0.13690358494096033	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1362	0.12076598619791966	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1349	0.11961330057341678	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1302	0.11544589870021396	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1286	0.11402720870082578	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1281	0.11358386807601695	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1262	0.11189917370174349	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1167	0.10347570183037609	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1163	0.10312102933052905	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1152	0.10214567995594967	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	1132	0.10037231745671443	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1131	0.10028364933175268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.866812496176187E-5	0.0
11	0.0	0.0	0.0	2.660043748852856E-4	0.0
12	0.0	0.0	0.0	0.0011526856245029043	0.0
13	0.0	0.0	0.0	0.0015960262493117136	0.0
14	0.0	0.0	0.0	0.0017733624992352375	0.0
15	0.0	0.0	0.0	0.0023940393739675706	0.0
16	0.0	0.0	0.0	0.00425606999816457	0.0
17	0.0	0.0	0.0	0.006384104997246854	0.0
18	0.0	0.0	0.0	0.007182118121902711	0.0
19	0.0	0.0	0.0	0.00851213999632914	0.0
20	0.0	0.0	0.0	0.009842161870755567	0.0
21	0.0	0.0	0.0	0.01569425811823185	0.0
22	0.0	0.0	0.0	0.02331971686494337	0.0
23	0.0	0.0	0.0	0.03972331998286932	0.0
24	0.0	0.0	0.0	0.06304303684781269	0.0
25	0.0	0.0	0.0	0.07137784059421831	0.0
26	0.0	0.0	0.0	0.0802446530903945	0.0
27	0.0	0.0	0.0	0.14488371618751889	0.0
28	0.0	0.0	0.0	0.21236015928341967	0.0
29	0.0	0.0	0.0	0.2937574979983171	0.0
30	0.0	0.0	0.0	0.4483947079316298	0.0
31	0.0	0.0	0.0	0.6610208715899347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4885	0.0	30.296827	1
CGATTGC	35	8.869293E-4	26.42857	31
ATTGGAC	435	0.0	22.540228	3
GTATTGG	440	0.0	22.28409	1
GTTCGCT	70	5.1017832E-6	21.142857	37
TAGTGCG	45	0.003825764	20.555557	7
AGTGCGC	65	6.9019836E-5	19.923077	8
GCCGTCA	65	6.9019836E-5	19.923077	13
TGGACCC	720	0.0	19.527779	5
GGACCCT	730	0.0	19.260273	6
TATTGGA	520	0.0	19.211538	2
TTGGACC	800	0.0	18.73125	4
GTATCAA	7930	0.0	18.616646	2
CTCTTAC	50	0.007034803	18.5	1
CTACGAT	50	0.007034803	18.5	1
TATAACG	50	0.007034803	18.5	2
GTGTAGA	115	6.40448E-8	17.695652	1
ACGATCT	95	3.6062647E-6	17.526316	32
TGTTCGC	95	3.6062647E-6	17.526316	36
CGTCTGT	310	0.0	17.306452	34
>>END_MODULE