FastQCFastQC Report
Wed 25 May 2016
SRR2088368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611114
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75331.2326669001201085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73021.194867078810173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64871.0615040728898373No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29130.476670473921396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.4229980003730892No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16430.2688532745117932No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT16230.2655805627100672No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT15860.259526045876874No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15060.24643519866996993No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA13640.2231989448777151No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT13080.21403535183288225No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT11370.18605366592812472No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG10600.1734537254914795No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9940.16265377654578358No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.1611810562350069No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9350.15299927673069183No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9300.15218109878026032No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9300.15218109878026032No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.11143583684877126No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC6810.11143583684877126No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6720.10996311653799454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA251.2329494E-436.99999629
ACGACGC303.597523E-430.83333428
GAACCGT250.00549435529.5999986
GTCGAAT405.9356476E-527.758
TCGAATC451.3222215E-424.6666689
GGTATCA20700.024.4879231
CGTGTCT551.900769E-523.54545435
TATCTCG400.001930158123.12536
TATGGCG652.6794332E-622.7692324
CGAATCG502.7000968E-422.19999910
TATACTG855.1706593E-821.7647065
CCCGTTG450.003823788120.55555731
TCTGTCG450.003823788120.5555578
ACCCGTT450.003823788120.55555730
CCGTTGA450.003823788120.55555732
AACCCGT555.139307E-420.18181829
GCACTAC555.139307E-420.18181830
GTCCTAC656.895373E-519.9230771
CTACCCT759.254134E-619.7333324
AGTCGAA609.230068E-418.57
Produced by FastQC (version 0.11.5)