FastQCFastQC Report
Wed 25 May 2016
SRR2088367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883689
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72380.8190664362688683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.7819493056946505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42330.47901467597763464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.29738969252757474No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.11033293387153172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.027.0537621
TACCGTC601.3368226E-624.6666667
ACGTTTA753.7387326E-722.226
CGAACGT502.7014175E-422.1999994
TCGAACG502.7014175E-422.1999993
GTATACT759.261794E-619.7333344
GTAGCAC500.00703362718.4999983
ACGACTT500.00703362718.49999821
GTATCAA27550.018.3992732
CGAACGA953.6044767E-617.52631616
GACCGTC953.6044767E-617.52631622
CGAATTA650.001579542917.07692315
CGGGCGT1107.802573E-716.8181826
TCTAGAC904.4440858E-516.4444453
GGGCGTC1151.2413238E-616.0869567
TATACGG1151.2413238E-616.0869562
ACGGACC1504.663889E-916.0333338
GGCGTCG1059.338128E-615.8571428
TCTACAC1751.3096724E-1015.8571423
CCGAATT700.00259185815.85714214
Produced by FastQC (version 0.11.5)