##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	883689
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.753030760821964	31.0	31.0	31.0	28.0	34.0
2	30.9837193854399	31.0	31.0	34.0	28.0	34.0
3	31.012744302577037	31.0	31.0	34.0	27.0	34.0
4	34.85094303538915	35.0	35.0	37.0	32.0	37.0
5	34.586619274427996	35.0	35.0	37.0	30.0	37.0
6	34.71895429274326	35.0	35.0	37.0	31.0	37.0
7	34.61844042417638	35.0	35.0	37.0	30.0	37.0
8	34.66976277853408	35.0	35.0	37.0	30.0	37.0
9	36.13454394023237	38.0	35.0	39.0	32.0	39.0
10	35.84340531567101	37.0	35.0	39.0	30.0	39.0
11	36.05689331880333	37.0	35.0	39.0	31.0	39.0
12	35.84294814125784	37.0	35.0	39.0	30.0	39.0
13	35.94672220656815	37.0	35.0	39.0	30.0	39.0
14	36.8416411203489	38.0	36.0	40.0	31.0	41.0
15	36.864075483569444	38.0	36.0	40.0	31.0	41.0
16	36.78200362344671	38.0	36.0	40.0	31.0	41.0
17	36.78133936260381	38.0	36.0	40.0	31.0	41.0
18	36.73470078274144	38.0	36.0	40.0	31.0	41.0
19	36.77869929352974	39.0	36.0	40.0	30.0	41.0
20	36.721809369585905	39.0	35.0	40.0	30.0	41.0
21	36.69502619134107	38.0	35.0	40.0	30.0	41.0
22	36.557705256034644	38.0	35.0	40.0	30.0	41.0
23	36.44370247903957	38.0	35.0	40.0	30.0	41.0
24	36.357354227561956	38.0	35.0	40.0	30.0	41.0
25	36.28232330604998	38.0	35.0	40.0	30.0	41.0
26	36.024962401931	38.0	34.0	40.0	29.0	41.0
27	35.827434764945586	38.0	34.0	40.0	28.0	41.0
28	35.71523918482634	38.0	34.0	40.0	27.0	41.0
29	35.55114412423375	38.0	34.0	40.0	27.0	41.0
30	35.3520639048353	38.0	34.0	40.0	27.0	41.0
31	35.160782809336766	38.0	34.0	40.0	27.0	41.0
32	35.008353617618866	37.0	33.0	40.0	26.0	41.0
33	35.16544395143541	38.0	33.0	40.0	26.0	41.0
34	35.17654061553329	38.0	33.0	40.0	26.0	41.0
35	35.125101704332636	38.0	33.0	40.0	26.0	41.0
36	35.04213246968108	38.0	33.0	40.0	26.0	41.0
37	34.93505294283396	38.0	33.0	40.0	25.0	41.0
38	34.840046668001975	38.0	33.0	40.0	25.0	41.0
39	34.823488806582404	38.0	33.0	40.0	25.0	41.0
40	34.702268558282384	38.0	33.0	40.0	24.0	41.0
41	34.60526950092171	38.0	33.0	40.0	24.0	41.0
42	34.49773958938043	38.0	33.0	40.0	24.0	41.0
43	34.114515400780135	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	8.0
16	27.0
17	64.0
18	118.0
19	258.0
20	532.0
21	1012.0
22	1768.0
23	2982.0
24	4781.0
25	7307.0
26	10315.0
27	14429.0
28	19605.0
29	25213.0
30	31661.0
31	38714.0
32	46307.0
33	55604.0
34	67376.0
35	81503.0
36	101652.0
37	130693.0
38	158975.0
39	82779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.43761775918904	25.12965534254698	14.64565022309885	21.78707667516513
2	21.202481868621202	25.6800752300866	31.78188254012441	21.335560361167786
3	22.307848123038763	26.553233094448387	30.670292376616658	20.46862640589619
4	16.882070502178934	19.446773695270622	34.56295144558776	29.108204356962688
5	13.040108001797012	40.08299299866808	34.27948067702552	12.597418322509391
6	31.42496964429794	38.846811491373096	15.427712690776959	14.300506173552009
7	25.141989998743902	34.07850499440414	23.15384711137063	17.62565789548133
8	26.076368496156455	35.38541274136037	20.670054736451398	17.86816402603178
9	25.77399967635673	13.97867349259751	22.24979602552482	37.997530805520945
10	15.946107736997972	28.46997077025967	34.83838771332449	20.745533779417872
11	33.74909046055796	23.185079818805033	23.098171415509302	19.96765830512771
12	19.424141298579027	28.130032171951896	31.629792834356884	20.816033695112193
13	29.527356343691054	21.611901924772177	27.355777881132386	21.504963850404383
14	20.844776838910523	22.847857108100246	28.25643410747446	28.05093194551477
15	23.562588195620858	30.139902160149102	24.537931331045197	21.75957831318484
16	22.317014243698857	28.28789313887578	26.991509456381145	22.403583161044214
17	20.789780114949945	28.566271618182416	28.245910043012866	22.398038223854773
18	21.118628838878838	27.05725656877023	30.066460032884873	21.757654559466054
19	21.460038542971567	27.070270196867902	30.980695697241906	20.48899556291863
20	22.599353392426522	26.377492534138142	30.307947705584205	20.71520636785113
21	22.649597313081866	26.73995036715405	29.61222783128454	20.998224488479543
22	22.257830526350332	26.834893271275302	30.01508449239495	20.892191709979414
23	21.68183603054921	27.16464729107186	30.02425061305505	21.129266065323886
24	21.211421665314383	27.236844636518047	29.950582161823903	21.601151536343668
25	21.864366309866934	27.34672492245575	29.919462616372954	20.86944615130436
26	21.75640977764802	27.47176891417682	29.451085166840368	21.32073614133479
27	21.83426522226711	27.54747428111021	29.358405502388287	21.2598549942344
28	21.510282463626908	27.7564844645571	29.71690266598317	21.01633040583282
29	21.172607104988295	27.475955907564764	30.087168675857683	21.26426831158926
30	21.232130308287193	27.79235681331328	30.091468831229086	20.88404404717044
31	21.75855985533372	27.43657553732139	29.785478827958705	21.019385779386187
32	21.614617812375165	27.646943664569772	29.384206434616704	21.354232088438355
33	21.66995402228612	27.612202935648174	29.44327698998177	21.274566052083934
34	21.81695143879804	27.644680424900614	29.604645978392853	20.933722157908495
35	21.540496713210192	27.71993314390017	29.84115452382003	20.898415619069606
36	21.338389410754235	27.22699954395721	29.971856614714	21.462754430574556
37	21.40538130496136	26.91342768779514	30.10527459321096	21.57591641403254
38	21.33782360083695	26.941491859692718	30.127228018001805	21.593456521468525
39	21.66655916278238	26.725012985337603	30.19614366592772	21.4122841859523
40	21.04326295789582	26.717657456412837	30.472145743581734	21.766933842109612
41	20.9479805678242	26.422983651488252	30.546153680763254	22.082882099924294
42	20.42732228193403	27.200859125778415	30.152689464279852	22.219129128007705
43	20.34494035797662	26.685066805176934	30.47327736341631	22.496715473430132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	181.0
1	197.0
2	213.0
3	376.0
4	539.0
5	539.0
6	968.0
7	1397.0
8	1476.5
9	1556.0
10	2355.5
11	3155.0
12	3155.0
13	5512.5
14	7870.0
15	11947.0
16	16024.0
17	15209.5
18	14395.0
19	14395.0
20	17138.0
21	19881.0
22	17653.0
23	15425.0
24	17939.5
25	20454.0
26	20454.0
27	22806.5
28	25159.0
29	26902.5
30	28646.0
31	31508.0
32	34370.0
33	34370.0
34	37939.5
35	41509.0
36	45016.0
37	48523.0
38	50410.0
39	52297.0
40	52297.0
41	54988.5
42	57680.0
43	59532.0
44	61384.0
45	64847.0
46	68310.0
47	68310.0
48	68546.5
49	68783.0
50	63942.0
51	59101.0
52	55653.0
53	52205.0
54	52205.0
55	47945.5
56	43686.0
57	40335.5
58	36985.0
59	33711.0
60	30437.0
61	30437.0
62	26781.0
63	23125.0
64	19938.0
65	16751.0
66	14177.5
67	11604.0
68	11604.0
69	9919.0
70	8234.0
71	7025.5
72	5817.0
73	4632.0
74	3447.0
75	3447.0
76	2663.0
77	1879.0
78	1492.0
79	1105.0
80	860.5
81	616.0
82	616.0
83	494.5
84	373.0
85	309.5
86	246.0
87	207.0
88	168.0
89	168.0
90	142.5
91	117.0
92	70.0
93	23.0
94	20.5
95	18.0
96	18.0
97	9.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	883689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.671719774578136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77651815022085	49.3941719497526
2	10.13217160750974	12.092082097425163
3	2.8788571073156115	5.153590637363694
4	1.2298770851492566	2.9355552312880566
5	0.6439450262472322	1.92126535782291
6	0.44274265456375705	1.5851529369228814
7	0.2860829089522723	1.1949741422707298
8	0.20532368221122918	0.9801613782394402
9	0.17094612676145188	0.9180584435392982
>10	1.0866746118684587	12.332011504492725
>50	0.10525543640693569	4.338556252045581
>100	0.03894588391816893	3.959020959593508
>500	0.0018997991965079767	0.8137288397541435
>1k	3.7995983930159537E-4	0.7777923613197518
>5k	3.7995983930159537E-4	1.6038779081696326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7238	0.8190664362688683	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6910	0.7819493056946505	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4233	0.47901467597763464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.29738969252757474	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	975	0.11033293387153172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.1316198345798125E-4	0.0
12	0.0	0.0	0.0	1.1316198345798125E-4	0.0
13	0.0	0.0	0.0	3.3948595037394376E-4	0.0
14	0.0	0.0	0.0	3.3948595037394376E-4	0.0
15	0.0	0.0	0.0	3.3948595037394376E-4	0.0
16	0.0	0.0	0.0	5.658099172899063E-4	0.0
17	0.0	0.0	0.0	9.0529586766385E-4	0.0
18	0.0	0.0	0.0	9.0529586766385E-4	0.0
19	0.0	0.0	0.0	0.0011316198345798125	0.0
20	0.0	0.0	0.0	0.001244781818037794	0.0
21	0.0	0.0	0.0	0.0018105917353277	0.0
22	0.0	0.0	0.0	0.0032816975202814565	0.0
23	0.0	0.0	0.0	0.0054317752059831	0.0
24	0.0	0.0	0.0	0.011316198345798126	0.0
25	0.0	0.0	0.0	0.015276867766827469	0.0
26	0.0	0.0	0.0	0.02150077685701644	0.0
27	0.0	0.0	0.0	0.07072623966123828	0.0
28	0.0	0.0	0.0	0.12108332230003994	0.0
29	0.0	0.0	0.0	0.1768721801448247	0.0
30	0.0	0.0	0.0	0.26479904129167614	0.0
31	0.0	0.0	0.0	0.4351078263959379	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1860	0.0	27.053762	1
TACCGTC	60	1.3368226E-6	24.666666	7
ACGTTTA	75	3.7387326E-7	22.2	26
CGAACGT	50	2.7014175E-4	22.199999	4
TCGAACG	50	2.7014175E-4	22.199999	3
GTATACT	75	9.261794E-6	19.733334	4
GTAGCAC	50	0.007033627	18.499998	3
ACGACTT	50	0.007033627	18.499998	21
GTATCAA	2755	0.0	18.399273	2
CGAACGA	95	3.6044767E-6	17.526316	16
GACCGTC	95	3.6044767E-6	17.526316	22
CGAATTA	65	0.0015795429	17.076923	15
CGGGCGT	110	7.802573E-7	16.818182	6
TCTAGAC	90	4.4440858E-5	16.444445	3
GGGCGTC	115	1.2413238E-6	16.086956	7
TATACGG	115	1.2413238E-6	16.086956	2
ACGGACC	150	4.663889E-9	16.033333	8
GGCGTCG	105	9.338128E-6	15.857142	8
TCTACAC	175	1.3096724E-10	15.857142	3
CCGAATT	70	0.002591858	15.857142	14
>>END_MODULE